Ultrastructure, chemical composition and biosynthesis of the cell wall in Koliella antarctica (Klebsormidiales, Chlorophyta)
The chemical composition, biosynthesis and ultrastructural organization of the cell wall of cultures of Koliella antarctica (Klebsormidiales, Chlorophyta), a green microalga obtained from samples of sea water taken near the Italian Station of Terra Nova (Ross Sea) during the austral summer 1989-1990...
Published in: | European Journal of Phycology |
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Format: | Article in Journal/Newspaper |
Language: | English |
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CAMBRIDGE UNIV PRESS, 40 WEST 20TH STREET, NEW YORK, NY 10011-4211 USA
2000
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Online Access: | http://hdl.handle.net/11577/2460748 https://doi.org/10.1080/09670260010001735931 |
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ftunivpadovairis:oai:www.research.unipd.it:11577/2460748 2024-04-14T08:02:50+00:00 Ultrastructure, chemical composition and biosynthesis of the cell wall in Koliella antarctica (Klebsormidiales, Chlorophyta) PIRO G. LENUCCI M. DALESSANDRO G. LA ROCCA, NICOLETTA RASCIO, NICOLETTA MORO, ISABELLA ANDREOLI, CARLO Piro, G. Lenucci, M. Dalessandro, G. LA ROCCA, Nicoletta Rascio, Nicoletta Moro, Isabella Andreoli, Carlo 2000 STAMPA http://hdl.handle.net/11577/2460748 https://doi.org/10.1080/09670260010001735931 eng eng CAMBRIDGE UNIV PRESS, 40 WEST 20TH STREET, NEW YORK, NY 10011-4211 USA info:eu-repo/semantics/altIdentifier/wos/WOS:000166621200003 volume:35 firstpage:331 lastpage:337 numberofpages:7 journal:EUROPEAN JOURNAL OF PHYCOLOGY http://hdl.handle.net/11577/2460748 doi:10.1080/09670260010001735931 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-0034493537 info:eu-repo/semantics/article 2000 ftunivpadovairis https://doi.org/10.1080/09670260010001735931 2024-03-21T19:07:11Z The chemical composition, biosynthesis and ultrastructural organization of the cell wall of cultures of Koliella antarctica (Klebsormidiales, Chlorophyta), a green microalga obtained from samples of sea water taken near the Italian Station of Terra Nova (Ross Sea) during the austral summer 1989-1990, have been studied. Purified cell walls of the microalga were sequentially treated with chemicals which, in higher plants, solubilize matrix polysaccharides (pectins and hemicelluloses) and leave an insoluble residue considered to be alpha -cellulose. CDTA plus Na2CO3- and KOH-solubilized polysaccharides were made up of glucosyl residues (more than 90 mol%), as well as some minor sugars (mannose and rhamnose). Linkage analysis and enzymic hydrolysis of the matrix polysaccharides indicated the presence of beta -1,4-linked glucans with minor amounts of other uncharacterized polysaccharides. The insoluble residue was composed of a small amount of crystalline alpha -cellulose associated with mannan or glucomannan chains. The biosynthesis of cell wall polysaccharides was studied by incubating the microalgae in the presence of D-[U-C-14]glucose or myo-[U-C-14]inositol. The radioactive glycosyl residues incorporated into the polysaccharides solubilized from purified cell walls of the cultured microalga corresponded to those detected in the chemical analysis of the cell wall. The myo-inositol oxidation pathway was demonstrated to be functional in the microalga. Cell wall ultrastructural observations showed a loose network of cellulose microfibrils in an extended matrix. Article in Journal/Newspaper Antarc* Antarctica Ross Sea Padua Research Archive (IRIS - Università degli Studi di Padova) Austral Ross Sea European Journal of Phycology 35 4 331 337 |
institution |
Open Polar |
collection |
Padua Research Archive (IRIS - Università degli Studi di Padova) |
op_collection_id |
ftunivpadovairis |
language |
English |
description |
The chemical composition, biosynthesis and ultrastructural organization of the cell wall of cultures of Koliella antarctica (Klebsormidiales, Chlorophyta), a green microalga obtained from samples of sea water taken near the Italian Station of Terra Nova (Ross Sea) during the austral summer 1989-1990, have been studied. Purified cell walls of the microalga were sequentially treated with chemicals which, in higher plants, solubilize matrix polysaccharides (pectins and hemicelluloses) and leave an insoluble residue considered to be alpha -cellulose. CDTA plus Na2CO3- and KOH-solubilized polysaccharides were made up of glucosyl residues (more than 90 mol%), as well as some minor sugars (mannose and rhamnose). Linkage analysis and enzymic hydrolysis of the matrix polysaccharides indicated the presence of beta -1,4-linked glucans with minor amounts of other uncharacterized polysaccharides. The insoluble residue was composed of a small amount of crystalline alpha -cellulose associated with mannan or glucomannan chains. The biosynthesis of cell wall polysaccharides was studied by incubating the microalgae in the presence of D-[U-C-14]glucose or myo-[U-C-14]inositol. The radioactive glycosyl residues incorporated into the polysaccharides solubilized from purified cell walls of the cultured microalga corresponded to those detected in the chemical analysis of the cell wall. The myo-inositol oxidation pathway was demonstrated to be functional in the microalga. Cell wall ultrastructural observations showed a loose network of cellulose microfibrils in an extended matrix. |
author2 |
Piro, G. Lenucci, M. Dalessandro, G. LA ROCCA, Nicoletta Rascio, Nicoletta Moro, Isabella Andreoli, Carlo |
format |
Article in Journal/Newspaper |
author |
PIRO G. LENUCCI M. DALESSANDRO G. LA ROCCA, NICOLETTA RASCIO, NICOLETTA MORO, ISABELLA ANDREOLI, CARLO |
spellingShingle |
PIRO G. LENUCCI M. DALESSANDRO G. LA ROCCA, NICOLETTA RASCIO, NICOLETTA MORO, ISABELLA ANDREOLI, CARLO Ultrastructure, chemical composition and biosynthesis of the cell wall in Koliella antarctica (Klebsormidiales, Chlorophyta) |
author_facet |
PIRO G. LENUCCI M. DALESSANDRO G. LA ROCCA, NICOLETTA RASCIO, NICOLETTA MORO, ISABELLA ANDREOLI, CARLO |
author_sort |
PIRO G. |
title |
Ultrastructure, chemical composition and biosynthesis of the cell wall in Koliella antarctica (Klebsormidiales, Chlorophyta) |
title_short |
Ultrastructure, chemical composition and biosynthesis of the cell wall in Koliella antarctica (Klebsormidiales, Chlorophyta) |
title_full |
Ultrastructure, chemical composition and biosynthesis of the cell wall in Koliella antarctica (Klebsormidiales, Chlorophyta) |
title_fullStr |
Ultrastructure, chemical composition and biosynthesis of the cell wall in Koliella antarctica (Klebsormidiales, Chlorophyta) |
title_full_unstemmed |
Ultrastructure, chemical composition and biosynthesis of the cell wall in Koliella antarctica (Klebsormidiales, Chlorophyta) |
title_sort |
ultrastructure, chemical composition and biosynthesis of the cell wall in koliella antarctica (klebsormidiales, chlorophyta) |
publisher |
CAMBRIDGE UNIV PRESS, 40 WEST 20TH STREET, NEW YORK, NY 10011-4211 USA |
publishDate |
2000 |
url |
http://hdl.handle.net/11577/2460748 https://doi.org/10.1080/09670260010001735931 |
geographic |
Austral Ross Sea |
geographic_facet |
Austral Ross Sea |
genre |
Antarc* Antarctica Ross Sea |
genre_facet |
Antarc* Antarctica Ross Sea |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000166621200003 volume:35 firstpage:331 lastpage:337 numberofpages:7 journal:EUROPEAN JOURNAL OF PHYCOLOGY http://hdl.handle.net/11577/2460748 doi:10.1080/09670260010001735931 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-0034493537 |
op_doi |
https://doi.org/10.1080/09670260010001735931 |
container_title |
European Journal of Phycology |
container_volume |
35 |
container_issue |
4 |
container_start_page |
331 |
op_container_end_page |
337 |
_version_ |
1796317883679113216 |