Biosynthesis and characterization of glycoproteins in Koliella antarctica (Klebsormidiales, Chlorophyta)
The biosynthesis of the oligosaccharide chains of glycoproteins in cultures of Koliella antarctica (Klebsormidiales), a green filamentous microalga collected from samples of seawater near the Italian station of Terra Nova (Ross Sea, Antarctica), has been studied by using D-[U-C-14] glucosamine as a...
Published in: | European Journal of Phycology |
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TAYLOR & FRANCIS LTD, 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND
2006
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Online Access: | http://hdl.handle.net/11577/1563673 https://doi.org/10.1080/09670260600648596 |
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ftunivpadovairis:oai:www.research.unipd.it:11577/1563673 2024-02-11T09:56:18+01:00 Biosynthesis and characterization of glycoproteins in Koliella antarctica (Klebsormidiales, Chlorophyta) LENUCCI M. LEUCCI M. R. DALESSANDRO G. PIRO G. ANDREOLI, CARLO Lenucci, M. Leucci, M. R. Andreoli, Carlo Dalessandro, G. Piro, G. 2006 STAMPA http://hdl.handle.net/11577/1563673 https://doi.org/10.1080/09670260600648596 eng eng TAYLOR & FRANCIS LTD, 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND info:eu-repo/semantics/altIdentifier/wos/WOS:000238286900008 volume:41 (2) firstpage:213 lastpage:222 numberofpages:10 journal:EUROPEAN JOURNAL OF PHYCOLOGY http://hdl.handle.net/11577/1563673 doi:10.1080/09670260600648596 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-33745014809 Antarctica D-glucosamine metabolism glycoprotein biosynthesi green microalga Koliella antarctica lectin binding info:eu-repo/semantics/article 2006 ftunivpadovairis https://doi.org/10.1080/09670260600648596 2024-01-24T17:35:24Z The biosynthesis of the oligosaccharide chains of glycoproteins in cultures of Koliella antarctica (Klebsormidiales), a green filamentous microalga collected from samples of seawater near the Italian station of Terra Nova (Ross Sea, Antarctica), has been studied by using D-[U-C-14] glucosamine as a tracer. To characterize the oligosaccharide moieties of Koliella glycoproteins, immunolabelling experiments with different lectins have also been performed. The use of radioactive tracer has shown that exogenously supplied D-[U-C-14] glucosamine is taken up by the microalga and sequentially metabolized to soluble radioactive UDP-N-acetylglucosamine and UDP-N-acetylgalactosamine. N-acetylglucosamine and N-acetylgalactosamine residues were incorporated mainly into the oligosaccharide chains of soluble and membrane-bound glycoproteins; the lowest amount of radioactive N-acetylhexosamines was detected in covalently and non-covalently linked cell-wall glycoproteins. Immunolabelling experiments with different lectins have shown similarities and differences among the patterns of soluble, membrane-bound and cell-wall glycoproteins. A peculiar characteristic of these microalgae is the presence of N-acetylgalactosamine residues as a component of the oligosaccharide chains of intracellular and cell wall N-linked glycoproteins. Article in Journal/Newspaper Antarc* Antarctica Ross Sea Padua Research Archive (IRIS - Università degli Studi di Padova) Ross Sea European Journal of Phycology 41 2 213 222 |
institution |
Open Polar |
collection |
Padua Research Archive (IRIS - Università degli Studi di Padova) |
op_collection_id |
ftunivpadovairis |
language |
English |
topic |
Antarctica D-glucosamine metabolism glycoprotein biosynthesi green microalga Koliella antarctica lectin binding |
spellingShingle |
Antarctica D-glucosamine metabolism glycoprotein biosynthesi green microalga Koliella antarctica lectin binding LENUCCI M. LEUCCI M. R. DALESSANDRO G. PIRO G. ANDREOLI, CARLO Biosynthesis and characterization of glycoproteins in Koliella antarctica (Klebsormidiales, Chlorophyta) |
topic_facet |
Antarctica D-glucosamine metabolism glycoprotein biosynthesi green microalga Koliella antarctica lectin binding |
description |
The biosynthesis of the oligosaccharide chains of glycoproteins in cultures of Koliella antarctica (Klebsormidiales), a green filamentous microalga collected from samples of seawater near the Italian station of Terra Nova (Ross Sea, Antarctica), has been studied by using D-[U-C-14] glucosamine as a tracer. To characterize the oligosaccharide moieties of Koliella glycoproteins, immunolabelling experiments with different lectins have also been performed. The use of radioactive tracer has shown that exogenously supplied D-[U-C-14] glucosamine is taken up by the microalga and sequentially metabolized to soluble radioactive UDP-N-acetylglucosamine and UDP-N-acetylgalactosamine. N-acetylglucosamine and N-acetylgalactosamine residues were incorporated mainly into the oligosaccharide chains of soluble and membrane-bound glycoproteins; the lowest amount of radioactive N-acetylhexosamines was detected in covalently and non-covalently linked cell-wall glycoproteins. Immunolabelling experiments with different lectins have shown similarities and differences among the patterns of soluble, membrane-bound and cell-wall glycoproteins. A peculiar characteristic of these microalgae is the presence of N-acetylgalactosamine residues as a component of the oligosaccharide chains of intracellular and cell wall N-linked glycoproteins. |
author2 |
Lenucci, M. Leucci, M. R. Andreoli, Carlo Dalessandro, G. Piro, G. |
format |
Article in Journal/Newspaper |
author |
LENUCCI M. LEUCCI M. R. DALESSANDRO G. PIRO G. ANDREOLI, CARLO |
author_facet |
LENUCCI M. LEUCCI M. R. DALESSANDRO G. PIRO G. ANDREOLI, CARLO |
author_sort |
LENUCCI M. |
title |
Biosynthesis and characterization of glycoproteins in Koliella antarctica (Klebsormidiales, Chlorophyta) |
title_short |
Biosynthesis and characterization of glycoproteins in Koliella antarctica (Klebsormidiales, Chlorophyta) |
title_full |
Biosynthesis and characterization of glycoproteins in Koliella antarctica (Klebsormidiales, Chlorophyta) |
title_fullStr |
Biosynthesis and characterization of glycoproteins in Koliella antarctica (Klebsormidiales, Chlorophyta) |
title_full_unstemmed |
Biosynthesis and characterization of glycoproteins in Koliella antarctica (Klebsormidiales, Chlorophyta) |
title_sort |
biosynthesis and characterization of glycoproteins in koliella antarctica (klebsormidiales, chlorophyta) |
publisher |
TAYLOR & FRANCIS LTD, 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND |
publishDate |
2006 |
url |
http://hdl.handle.net/11577/1563673 https://doi.org/10.1080/09670260600648596 |
geographic |
Ross Sea |
geographic_facet |
Ross Sea |
genre |
Antarc* Antarctica Ross Sea |
genre_facet |
Antarc* Antarctica Ross Sea |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000238286900008 volume:41 (2) firstpage:213 lastpage:222 numberofpages:10 journal:EUROPEAN JOURNAL OF PHYCOLOGY http://hdl.handle.net/11577/1563673 doi:10.1080/09670260600648596 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-33745014809 |
op_doi |
https://doi.org/10.1080/09670260600648596 |
container_title |
European Journal of Phycology |
container_volume |
41 |
container_issue |
2 |
container_start_page |
213 |
op_container_end_page |
222 |
_version_ |
1790602078748409856 |