Histochemical study of glycoconjugate secretions in the gut of Anguilla anguilla L
Conventional histochemical methods as well as lectin-binding techniques were used to study glycoconjugates that are present in the alimentary canal of the European eel (Anguilla anguilla). Specimens from pharynx, oesophagus, stomach and intestine were collected from adult ("silver eel" sta...
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ftunivpadovairis:oai:www.research.unipd.it:11577/1426550 2023-09-05T13:11:55+02:00 Histochemical study of glycoconjugate secretions in the gut of Anguilla anguilla L DOMENEGHINI C. ARRIGHI S. VEGGETTI A. RADAELLI, GIUSEPPE Domeneghini, C. Arrighi, S. Radaelli, Giuseppe Veggetti, A. 2005 http://hdl.handle.net/11577/1426550 unknown info:eu-repo/semantics/altIdentifier/pmid/20483292 volume:106 firstpage:477 lastpage:487 journal:ACTA HISTOCHEMICA http://hdl.handle.net/11577/1426550 info:eu-repo/semantics/article 2005 ftunivpadovairis 2023-08-21T19:43:22Z Conventional histochemical methods as well as lectin-binding techniques were used to study glycoconjugates that are present in the alimentary canal of the European eel (Anguilla anguilla). Specimens from pharynx, oesophagus, stomach and intestine were collected from adult ("silver eel" stage) females. Alcian Blue pH 2.5/PAS and High Iron Diamine/Alcian Blue pH 2.5 reactions were performed to stain neutral and acidic glycoconjugates. In addition, lectin histochemistry was applied to identify acidic glycoconjugates containing O-acylated sialic acids. Finally, the presence of sugar residues in the oligosaccharide side chains of glycoconjugates were investigated by using biotinylated lectins. Acidic and neutral glycoconjugates were found to be secreted throughout the alimentary canal, the acidic glycoconjugates appeared to be either sialylated or sulphated. Sialylated glycoconjugates were identified to contain sialic acid substituted at carbon in position 7 (C7). Sulphated glycoconjugates were particularly abundant in the distal intestine and were not present in the secretory products of the gastric mucosa, which contained a variety of sugar residues (D-N-acetyl-galactosamine, beta-D-galactose, alpha-D-mannose, alpha-L-fucose, D-N-acetyl-glucosamine). Lectin binding was observed in mucous cells of pharynx, oesophagus and intestine, and particularly some monosaccharides (D-N-acetyl-galactosamine and beta-D-galactose) were abundantly present. Article in Journal/Newspaper Anguilla anguilla Padua Research Archive (IRIS - Università degli Studi di Padova) |
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Padua Research Archive (IRIS - Università degli Studi di Padova) |
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Conventional histochemical methods as well as lectin-binding techniques were used to study glycoconjugates that are present in the alimentary canal of the European eel (Anguilla anguilla). Specimens from pharynx, oesophagus, stomach and intestine were collected from adult ("silver eel" stage) females. Alcian Blue pH 2.5/PAS and High Iron Diamine/Alcian Blue pH 2.5 reactions were performed to stain neutral and acidic glycoconjugates. In addition, lectin histochemistry was applied to identify acidic glycoconjugates containing O-acylated sialic acids. Finally, the presence of sugar residues in the oligosaccharide side chains of glycoconjugates were investigated by using biotinylated lectins. Acidic and neutral glycoconjugates were found to be secreted throughout the alimentary canal, the acidic glycoconjugates appeared to be either sialylated or sulphated. Sialylated glycoconjugates were identified to contain sialic acid substituted at carbon in position 7 (C7). Sulphated glycoconjugates were particularly abundant in the distal intestine and were not present in the secretory products of the gastric mucosa, which contained a variety of sugar residues (D-N-acetyl-galactosamine, beta-D-galactose, alpha-D-mannose, alpha-L-fucose, D-N-acetyl-glucosamine). Lectin binding was observed in mucous cells of pharynx, oesophagus and intestine, and particularly some monosaccharides (D-N-acetyl-galactosamine and beta-D-galactose) were abundantly present. |
author2 |
Domeneghini, C. Arrighi, S. Radaelli, Giuseppe Veggetti, A. |
format |
Article in Journal/Newspaper |
author |
DOMENEGHINI C. ARRIGHI S. VEGGETTI A. RADAELLI, GIUSEPPE |
spellingShingle |
DOMENEGHINI C. ARRIGHI S. VEGGETTI A. RADAELLI, GIUSEPPE Histochemical study of glycoconjugate secretions in the gut of Anguilla anguilla L |
author_facet |
DOMENEGHINI C. ARRIGHI S. VEGGETTI A. RADAELLI, GIUSEPPE |
author_sort |
DOMENEGHINI C. |
title |
Histochemical study of glycoconjugate secretions in the gut of Anguilla anguilla L |
title_short |
Histochemical study of glycoconjugate secretions in the gut of Anguilla anguilla L |
title_full |
Histochemical study of glycoconjugate secretions in the gut of Anguilla anguilla L |
title_fullStr |
Histochemical study of glycoconjugate secretions in the gut of Anguilla anguilla L |
title_full_unstemmed |
Histochemical study of glycoconjugate secretions in the gut of Anguilla anguilla L |
title_sort |
histochemical study of glycoconjugate secretions in the gut of anguilla anguilla l |
publishDate |
2005 |
url |
http://hdl.handle.net/11577/1426550 |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/20483292 volume:106 firstpage:477 lastpage:487 journal:ACTA HISTOCHEMICA http://hdl.handle.net/11577/1426550 |
_version_ |
1776197915695382528 |