Finding 16S rRNA gene-based SNPs for the genetic traceability of commercial species belonging to Gadiformes
A SNPs (Single Nucleotide Polymorphism) based analysis was developed to differentiate four economically important species belonging to the Gadiformes order: Pacific cod Gadus macrocephalus, Atlantic cod Gadus morhua, Haddock Melanogrammus aeglefinus and Ling Molva molva. A 430bp fragment of the 16s...
Published in: | Italian Journal of Animal Science |
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Format: | Article in Journal/Newspaper |
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PAvia: PAGE Press - MeditGroup Bologna Italy: Avenue Media
2007
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Online Access: | http://hdl.handle.net/11577/123084 https://doi.org/10.4081/ijas.2007.1s.161 |
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ftunivpadovairis:oai:www.research.unipd.it:11577/123084 2024-02-27T08:38:34+00:00 Finding 16S rRNA gene-based SNPs for the genetic traceability of commercial species belonging to Gadiformes MARETTO F REFFO E DALVIT C BARCACCIA, GIANNI MANTOVANI, ROBERTO Maretto, F Reffo, E Dalvit, C Barcaccia, Gianni Mantovani, Roberto 2007 http://hdl.handle.net/11577/123084 https://doi.org/10.4081/ijas.2007.1s.161 unknown PAvia: PAGE Press - MeditGroup Bologna Italy: Avenue Media info:eu-repo/semantics/altIdentifier/wos/WOS:000248276300063 volume:6(1s) firstpage:161 lastpage:163 numberofpages:3 journal:ITALIAN JOURNAL OF ANIMAL SCIENCE http://hdl.handle.net/11577/123084 doi:10.4081/ijas.2007.1s.161 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-34250862009 info:eu-repo/semantics/article 2007 ftunivpadovairis https://doi.org/10.4081/ijas.2007.1s.161 2024-01-31T17:34:51Z A SNPs (Single Nucleotide Polymorphism) based analysis was developed to differentiate four economically important species belonging to the Gadiformes order: Pacific cod Gadus macrocephalus, Atlantic cod Gadus morhua, Haddock Melanogrammus aeglefinus and Ling Molva molva. A 430bp fragment of the 16s rRNA gene was amplified using interspecific conserved primer and sequenced. The sequences were aligned and analyzed for the presence of SNPs; three SNPs (MerSNP1, MerSNP7 and MerSNP9) were identified and selected to allow discrimination between the four species. Aplotypes were TCC, CCC, CAT and CAC for Pacific cod, Atlantic cod, Haddock and Ling respectively. Confirmation of results was achieved by sequencing 16s rRNA gene fragments of 16 G. morhua, 7 G. macrocephalus, 15 M. aeglefinus and 5 M. molva samples collected at different fish catching campaign. Nucleotide sequence of 16s rRNA mitochondial gene has been shown to be a useful tool to allow rapid reliable and fully automatable for discrimination of 4 economically important species in fisheries industry. Article in Journal/Newspaper atlantic cod Gadus morhua Padua Research Archive (IRIS - Università degli Studi di Padova) Pacific Italian Journal of Animal Science 6 sup1 161 163 |
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Open Polar |
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Padua Research Archive (IRIS - Università degli Studi di Padova) |
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ftunivpadovairis |
language |
unknown |
description |
A SNPs (Single Nucleotide Polymorphism) based analysis was developed to differentiate four economically important species belonging to the Gadiformes order: Pacific cod Gadus macrocephalus, Atlantic cod Gadus morhua, Haddock Melanogrammus aeglefinus and Ling Molva molva. A 430bp fragment of the 16s rRNA gene was amplified using interspecific conserved primer and sequenced. The sequences were aligned and analyzed for the presence of SNPs; three SNPs (MerSNP1, MerSNP7 and MerSNP9) were identified and selected to allow discrimination between the four species. Aplotypes were TCC, CCC, CAT and CAC for Pacific cod, Atlantic cod, Haddock and Ling respectively. Confirmation of results was achieved by sequencing 16s rRNA gene fragments of 16 G. morhua, 7 G. macrocephalus, 15 M. aeglefinus and 5 M. molva samples collected at different fish catching campaign. Nucleotide sequence of 16s rRNA mitochondial gene has been shown to be a useful tool to allow rapid reliable and fully automatable for discrimination of 4 economically important species in fisheries industry. |
author2 |
Maretto, F Reffo, E Dalvit, C Barcaccia, Gianni Mantovani, Roberto |
format |
Article in Journal/Newspaper |
author |
MARETTO F REFFO E DALVIT C BARCACCIA, GIANNI MANTOVANI, ROBERTO |
spellingShingle |
MARETTO F REFFO E DALVIT C BARCACCIA, GIANNI MANTOVANI, ROBERTO Finding 16S rRNA gene-based SNPs for the genetic traceability of commercial species belonging to Gadiformes |
author_facet |
MARETTO F REFFO E DALVIT C BARCACCIA, GIANNI MANTOVANI, ROBERTO |
author_sort |
MARETTO F |
title |
Finding 16S rRNA gene-based SNPs for the genetic traceability of commercial species belonging to Gadiformes |
title_short |
Finding 16S rRNA gene-based SNPs for the genetic traceability of commercial species belonging to Gadiformes |
title_full |
Finding 16S rRNA gene-based SNPs for the genetic traceability of commercial species belonging to Gadiformes |
title_fullStr |
Finding 16S rRNA gene-based SNPs for the genetic traceability of commercial species belonging to Gadiformes |
title_full_unstemmed |
Finding 16S rRNA gene-based SNPs for the genetic traceability of commercial species belonging to Gadiformes |
title_sort |
finding 16s rrna gene-based snps for the genetic traceability of commercial species belonging to gadiformes |
publisher |
PAvia: PAGE Press - MeditGroup Bologna Italy: Avenue Media |
publishDate |
2007 |
url |
http://hdl.handle.net/11577/123084 https://doi.org/10.4081/ijas.2007.1s.161 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000248276300063 volume:6(1s) firstpage:161 lastpage:163 numberofpages:3 journal:ITALIAN JOURNAL OF ANIMAL SCIENCE http://hdl.handle.net/11577/123084 doi:10.4081/ijas.2007.1s.161 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-34250862009 |
op_doi |
https://doi.org/10.4081/ijas.2007.1s.161 |
container_title |
Italian Journal of Animal Science |
container_volume |
6 |
container_issue |
sup1 |
container_start_page |
161 |
op_container_end_page |
163 |
_version_ |
1792045470529355776 |