Development of a biomarker panel for identifying stressed marine mammals
Increasing anthropogenic disturbance in marine ecosystems such as fishing, oil-drilling, and noise pollution can have detrimental effects on the reproduction and survival of apex predators such as marine mammals. Stress activates the hypothalamic-pituitary-adrenal (HPA) axis, resulting in increased...
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ftunivpacificdc:oai:scholarlycommons.pacific.edu:uop_etds-4590 2023-08-27T04:09:15+02:00 Development of a biomarker panel for identifying stressed marine mammals Pujade, Laura 2019-01-01T08:00:00Z application/pdf https://scholarlycommons.pacific.edu/uop_etds/3587 https://scholarlycommons.pacific.edu/context/uop_etds/article/4590/viewcontent/Pujade_pacific_0173N_10491.pdf unknown Scholarly Commons https://scholarlycommons.pacific.edu/uop_etds/3587 https://scholarlycommons.pacific.edu/context/uop_etds/article/4590/viewcontent/Pujade_pacific_0173N_10491.pdf University of the Pacific Theses and Dissertations Biology Molecular biology Physiology Biochemistry Biophysics and Structural Biology Marine Biology text 2019 ftunivpacificdc 2023-08-07T21:43:09Z Increasing anthropogenic disturbance in marine ecosystems such as fishing, oil-drilling, and noise pollution can have detrimental effects on the reproduction and survival of apex predators such as marine mammals. Stress activates the hypothalamic-pituitary-adrenal (HPA) axis, resulting in increased circulating glucocorticoid (GCs) hormones, which alter expression of target genes encoding metabolic enzymes and other mediators of stress. Prolonged HPA axis stimulation may increase catabolism of nutrient stores and suppress immune and reproductive functions, impacting the fitness of marine mammals. GCs measurements are used to identify wild animals experiencing stress. However, these measurements may not be sensitive enough to distinguish between an acutely and a chronically stressed individuals. In this study, we present a new approach of assessing stress states in marine mammals, by measuring expression levels of gene markers in blubber. We previously characterized transcriptional and metabolic profiles and identified genes and metabolites that were differentially expressed in response to single and repeated adrenocorticotropic hormone (ACTH) administration in juvenile northern elephant seals. We then measured expression of these target genes in blubber tissue collected from juvenile northern elephant seals in their natural baseline stress states (n=30), and correlated their gene expression values with cortisol, aldosterone, total triiodothyronine (tT3), reverse triiodothyronine (rT3), and triglyceride levels, and body condition index. We found that blubber genes that were upregulated in response to repeated ACTH administration in the previous study were positively correlated with cortisol and inversely correlated with tT3 in the baseline sample set. These markers included genes that encode a lipid particle protein (PLIN1), an adipogenesis promoting transcription factor (DKK1), an oxidative stress enzyme (GPX3), and a lipid metabolism enzyme (AZGP1). Blubber genes differentially expressed in response to acute ... Text Elephant Seals University of the Pacific: Scholarly Commons |
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Biology Molecular biology Physiology Biochemistry Biophysics and Structural Biology Marine Biology |
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Biology Molecular biology Physiology Biochemistry Biophysics and Structural Biology Marine Biology Pujade, Laura Development of a biomarker panel for identifying stressed marine mammals |
topic_facet |
Biology Molecular biology Physiology Biochemistry Biophysics and Structural Biology Marine Biology |
description |
Increasing anthropogenic disturbance in marine ecosystems such as fishing, oil-drilling, and noise pollution can have detrimental effects on the reproduction and survival of apex predators such as marine mammals. Stress activates the hypothalamic-pituitary-adrenal (HPA) axis, resulting in increased circulating glucocorticoid (GCs) hormones, which alter expression of target genes encoding metabolic enzymes and other mediators of stress. Prolonged HPA axis stimulation may increase catabolism of nutrient stores and suppress immune and reproductive functions, impacting the fitness of marine mammals. GCs measurements are used to identify wild animals experiencing stress. However, these measurements may not be sensitive enough to distinguish between an acutely and a chronically stressed individuals. In this study, we present a new approach of assessing stress states in marine mammals, by measuring expression levels of gene markers in blubber. We previously characterized transcriptional and metabolic profiles and identified genes and metabolites that were differentially expressed in response to single and repeated adrenocorticotropic hormone (ACTH) administration in juvenile northern elephant seals. We then measured expression of these target genes in blubber tissue collected from juvenile northern elephant seals in their natural baseline stress states (n=30), and correlated their gene expression values with cortisol, aldosterone, total triiodothyronine (tT3), reverse triiodothyronine (rT3), and triglyceride levels, and body condition index. We found that blubber genes that were upregulated in response to repeated ACTH administration in the previous study were positively correlated with cortisol and inversely correlated with tT3 in the baseline sample set. These markers included genes that encode a lipid particle protein (PLIN1), an adipogenesis promoting transcription factor (DKK1), an oxidative stress enzyme (GPX3), and a lipid metabolism enzyme (AZGP1). Blubber genes differentially expressed in response to acute ... |
format |
Text |
author |
Pujade, Laura |
author_facet |
Pujade, Laura |
author_sort |
Pujade, Laura |
title |
Development of a biomarker panel for identifying stressed marine mammals |
title_short |
Development of a biomarker panel for identifying stressed marine mammals |
title_full |
Development of a biomarker panel for identifying stressed marine mammals |
title_fullStr |
Development of a biomarker panel for identifying stressed marine mammals |
title_full_unstemmed |
Development of a biomarker panel for identifying stressed marine mammals |
title_sort |
development of a biomarker panel for identifying stressed marine mammals |
publisher |
Scholarly Commons |
publishDate |
2019 |
url |
https://scholarlycommons.pacific.edu/uop_etds/3587 https://scholarlycommons.pacific.edu/context/uop_etds/article/4590/viewcontent/Pujade_pacific_0173N_10491.pdf |
genre |
Elephant Seals |
genre_facet |
Elephant Seals |
op_source |
University of the Pacific Theses and Dissertations |
op_relation |
https://scholarlycommons.pacific.edu/uop_etds/3587 https://scholarlycommons.pacific.edu/context/uop_etds/article/4590/viewcontent/Pujade_pacific_0173N_10491.pdf |
_version_ |
1775350425112805376 |