Salmon cardiac peptide (sCP): a new model for natriuretic peptide biology
Abstract Natriuretic peptide hormones secreted from the heart are important in maintaining the volume and electrolyte balance and in regulation of blood pressure. The secretion of natriuretic peptides is stimulated by myocyte stretch and paracrine factors. However, the intracellular actions of these...
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University of Oulu
2001
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ftunivoulu:oai:oulu.fi:isbn951-42-6493-2 2023-07-30T04:06:36+02:00 Salmon cardiac peptide (sCP): a new model for natriuretic peptide biology Tervonen, V. (Virpi) 2001-09-18 application/pdf http://urn.fi/urn:isbn:9514264932 eng eng University of Oulu info:eu-repo/semantics/altIdentifier/pissn/0355-3221 info:eu-repo/semantics/altIdentifier/eissn/1796-2234 info:eu-repo/semantics/openAccess © University of Oulu, 2001 elimination mechanical load osmoregulation temperature info:eu-repo/semantics/doctoralThesis info:eu-repo/semantics/publishedVersion 2001 ftunivoulu 2023-07-08T20:01:14Z Abstract Natriuretic peptide hormones secreted from the heart are important in maintaining the volume and electrolyte balance and in regulation of blood pressure. The secretion of natriuretic peptides is stimulated by myocyte stretch and paracrine factors. However, the intracellular actions of these stimuli and the cellular and molecular mechanisms involved in the processing and secretion of natriuretic peptides are still largely unknown. In this study, a new model for studies of the natriuretic peptide system was developed using a novel natriuretic peptide from salmon. Salmon (Salmo salar) maintains its water and salt homeostasis despite the volume gain in fresh water and electrolyte gain in sea water. Thus, salmon is an ideal model to study the mechanisms regulating the extracellular volume and salt balance, like natriuretic peptides. Furthermore, comparative studies revealing the common characteristics in phylogenetically distinct species suggest the importance of these factors in the regulation of the natriuretic peptide system. A novel natriuretic peptide, salmon cardiac peptide (sCP), was cloned from salmon heart. Distribution of sCP was studied in a variety of vertebrates and its physiological effects were examined in in vitro and in vivo experiments in salmon and rats. The storage and release of sCP was studied using a salmon ventricle perfusion system and by analysing the molecular forms of stored and secreted forms. Factors modulating the secretion and circulating concentration of sCP, and cardiac peptide and sCP mRNA level in salmon were examined as well. The biosynthesis of sCP is strictly restricted to the heart. sCP is stored in myocytes in the prohormone form, while the secreted form is a 29-amino acid peptide in salmon. Mechanical load on isolated salmon ventricle and volume overload in intact salmon induced a rapid release of sCP. Exposure to hyperosmotic environment decreased the plasma sCP level. sCP increased diuresis and natriuresis, as well as relaxed preconstricted arteries from salmon and ... Doctoral or Postdoctoral Thesis Salmo salar Jultika - University of Oulu repository |
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Jultika - University of Oulu repository |
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ftunivoulu |
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English |
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elimination mechanical load osmoregulation temperature |
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elimination mechanical load osmoregulation temperature Tervonen, V. (Virpi) Salmon cardiac peptide (sCP): a new model for natriuretic peptide biology |
topic_facet |
elimination mechanical load osmoregulation temperature |
description |
Abstract Natriuretic peptide hormones secreted from the heart are important in maintaining the volume and electrolyte balance and in regulation of blood pressure. The secretion of natriuretic peptides is stimulated by myocyte stretch and paracrine factors. However, the intracellular actions of these stimuli and the cellular and molecular mechanisms involved in the processing and secretion of natriuretic peptides are still largely unknown. In this study, a new model for studies of the natriuretic peptide system was developed using a novel natriuretic peptide from salmon. Salmon (Salmo salar) maintains its water and salt homeostasis despite the volume gain in fresh water and electrolyte gain in sea water. Thus, salmon is an ideal model to study the mechanisms regulating the extracellular volume and salt balance, like natriuretic peptides. Furthermore, comparative studies revealing the common characteristics in phylogenetically distinct species suggest the importance of these factors in the regulation of the natriuretic peptide system. A novel natriuretic peptide, salmon cardiac peptide (sCP), was cloned from salmon heart. Distribution of sCP was studied in a variety of vertebrates and its physiological effects were examined in in vitro and in vivo experiments in salmon and rats. The storage and release of sCP was studied using a salmon ventricle perfusion system and by analysing the molecular forms of stored and secreted forms. Factors modulating the secretion and circulating concentration of sCP, and cardiac peptide and sCP mRNA level in salmon were examined as well. The biosynthesis of sCP is strictly restricted to the heart. sCP is stored in myocytes in the prohormone form, while the secreted form is a 29-amino acid peptide in salmon. Mechanical load on isolated salmon ventricle and volume overload in intact salmon induced a rapid release of sCP. Exposure to hyperosmotic environment decreased the plasma sCP level. sCP increased diuresis and natriuresis, as well as relaxed preconstricted arteries from salmon and ... |
format |
Doctoral or Postdoctoral Thesis |
author |
Tervonen, V. (Virpi) |
author_facet |
Tervonen, V. (Virpi) |
author_sort |
Tervonen, V. (Virpi) |
title |
Salmon cardiac peptide (sCP): a new model for natriuretic peptide biology |
title_short |
Salmon cardiac peptide (sCP): a new model for natriuretic peptide biology |
title_full |
Salmon cardiac peptide (sCP): a new model for natriuretic peptide biology |
title_fullStr |
Salmon cardiac peptide (sCP): a new model for natriuretic peptide biology |
title_full_unstemmed |
Salmon cardiac peptide (sCP): a new model for natriuretic peptide biology |
title_sort |
salmon cardiac peptide (scp): a new model for natriuretic peptide biology |
publisher |
University of Oulu |
publishDate |
2001 |
url |
http://urn.fi/urn:isbn:9514264932 |
genre |
Salmo salar |
genre_facet |
Salmo salar |
op_relation |
info:eu-repo/semantics/altIdentifier/pissn/0355-3221 info:eu-repo/semantics/altIdentifier/eissn/1796-2234 |
op_rights |
info:eu-repo/semantics/openAccess © University of Oulu, 2001 |
_version_ |
1772819299385212928 |