Chemical and Microbial Characterization of North Slope Viscous Oils to Assess Viscosity Reduction and Enhanced Recovery
A large proportion of Alaska North Slope (ANS) oil exists in the form of viscous deposits, which cannot be produced entirely using conventional methods. Microbially enhanced oil recovery (MEOR) is a promising approach for improving oil recovery for viscous deposits. MEOR can be achieved using either...
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ftunivnotexas:info:ark/67531/metadc933863 2023-05-15T13:09:12+02:00 Chemical and Microbial Characterization of North Slope Viscous Oils to Assess Viscosity Reduction and Enhanced Recovery Patil, Shirish Dandekar, Abhijit Leigh, Mary Beth United States. Department of Energy. 2008-12-31 Text https://doi.org/10.2172/963366 https://digital.library.unt.edu/ark:/67531/metadc933863/ English eng University of Alaska grantno: FC26-01NT41248 doi:10.2172/963366 osti: 963366 https://digital.library.unt.edu/ark:/67531/metadc933863/ ark: ark:/67531/metadc933863 Alaskan North Slope 02 Petroleum Microorganisms Genetics Testing Chemical Composition Petroleum Bacillus Licheniformis Enhanced Recovery Surface Tension Strains Oil Fields Surfactants Viscosity Bacteria Oil Spills Communities Report 2008 ftunivnotexas https://doi.org/10.2172/963366 2019-05-18T22:08:18Z A large proportion of Alaska North Slope (ANS) oil exists in the form of viscous deposits, which cannot be produced entirely using conventional methods. Microbially enhanced oil recovery (MEOR) is a promising approach for improving oil recovery for viscous deposits. MEOR can be achieved using either ex situ approaches such as flooding with microbial biosurfactants or injection of exogenous surfactant-producing microbes into the reservoir, or by in situ approaches such as biostimulation of indigenous surfactant-producing microbes in the oil. Experimental work was performed to analyze the potential application of MEOR to the ANS oil fields through both ex situ and in situ approaches. A microbial formulation containing a known biosurfactant-producing strain of Bacillus licheniformis was developed in order to simulate MEOR. Coreflooding experiments were performed to simulate MEOR and quantify the incremental oil recovery. Properties like viscosity, density, and chemical composition of oil were monitored to propose a mechanism for oil recovery. The microbial formulation significantly increased incremental oil recovery, and molecular biological analyses indicated that the strain survived during the shut-in period. The indigenous microflora of ANS heavy oils was investigated to characterize the microbial communities and test for surfactant producers that are potentially useful for biostimulation. Bacteria that reduce the surface tension of aqueous media were isolated from one of the five ANS oils (Milne Point) and from rock oiled by the Exxon Valdez oil spill (EVOS), and may prove valuable for ex situ MEOR strategies. The total bacterial community composition of the six different oils was evaluated using molecular genetic tools, which revealed that each oil tested possessed a unique fingerprint indicating a diverse bacterial community and varied assemblages. Collectively we have demonstrated that there is potential for in situ and ex situ MEOR of ANS oils. Future work should focus on lab and field-scale testing of ex situ MEOR using Bacillus licheniformis as well as the biosurfactant-producing strains we have newly isolated from the Milne Point reservoir and the EVOS environment. Report Alaska North Slope north slope Alaska University of North Texas: UNT Digital Library Milne Point ENVELOPE(-100.852,-100.852,73.835,73.835) |
institution |
Open Polar |
collection |
University of North Texas: UNT Digital Library |
op_collection_id |
ftunivnotexas |
language |
English |
topic |
Alaskan North Slope 02 Petroleum Microorganisms Genetics Testing Chemical Composition Petroleum Bacillus Licheniformis Enhanced Recovery Surface Tension Strains Oil Fields Surfactants Viscosity Bacteria Oil Spills Communities |
spellingShingle |
Alaskan North Slope 02 Petroleum Microorganisms Genetics Testing Chemical Composition Petroleum Bacillus Licheniformis Enhanced Recovery Surface Tension Strains Oil Fields Surfactants Viscosity Bacteria Oil Spills Communities Patil, Shirish Dandekar, Abhijit Leigh, Mary Beth Chemical and Microbial Characterization of North Slope Viscous Oils to Assess Viscosity Reduction and Enhanced Recovery |
topic_facet |
Alaskan North Slope 02 Petroleum Microorganisms Genetics Testing Chemical Composition Petroleum Bacillus Licheniformis Enhanced Recovery Surface Tension Strains Oil Fields Surfactants Viscosity Bacteria Oil Spills Communities |
description |
A large proportion of Alaska North Slope (ANS) oil exists in the form of viscous deposits, which cannot be produced entirely using conventional methods. Microbially enhanced oil recovery (MEOR) is a promising approach for improving oil recovery for viscous deposits. MEOR can be achieved using either ex situ approaches such as flooding with microbial biosurfactants or injection of exogenous surfactant-producing microbes into the reservoir, or by in situ approaches such as biostimulation of indigenous surfactant-producing microbes in the oil. Experimental work was performed to analyze the potential application of MEOR to the ANS oil fields through both ex situ and in situ approaches. A microbial formulation containing a known biosurfactant-producing strain of Bacillus licheniformis was developed in order to simulate MEOR. Coreflooding experiments were performed to simulate MEOR and quantify the incremental oil recovery. Properties like viscosity, density, and chemical composition of oil were monitored to propose a mechanism for oil recovery. The microbial formulation significantly increased incremental oil recovery, and molecular biological analyses indicated that the strain survived during the shut-in period. The indigenous microflora of ANS heavy oils was investigated to characterize the microbial communities and test for surfactant producers that are potentially useful for biostimulation. Bacteria that reduce the surface tension of aqueous media were isolated from one of the five ANS oils (Milne Point) and from rock oiled by the Exxon Valdez oil spill (EVOS), and may prove valuable for ex situ MEOR strategies. The total bacterial community composition of the six different oils was evaluated using molecular genetic tools, which revealed that each oil tested possessed a unique fingerprint indicating a diverse bacterial community and varied assemblages. Collectively we have demonstrated that there is potential for in situ and ex situ MEOR of ANS oils. Future work should focus on lab and field-scale testing of ex situ MEOR using Bacillus licheniformis as well as the biosurfactant-producing strains we have newly isolated from the Milne Point reservoir and the EVOS environment. |
author2 |
United States. Department of Energy. |
format |
Report |
author |
Patil, Shirish Dandekar, Abhijit Leigh, Mary Beth |
author_facet |
Patil, Shirish Dandekar, Abhijit Leigh, Mary Beth |
author_sort |
Patil, Shirish |
title |
Chemical and Microbial Characterization of North Slope Viscous Oils to Assess Viscosity Reduction and Enhanced Recovery |
title_short |
Chemical and Microbial Characterization of North Slope Viscous Oils to Assess Viscosity Reduction and Enhanced Recovery |
title_full |
Chemical and Microbial Characterization of North Slope Viscous Oils to Assess Viscosity Reduction and Enhanced Recovery |
title_fullStr |
Chemical and Microbial Characterization of North Slope Viscous Oils to Assess Viscosity Reduction and Enhanced Recovery |
title_full_unstemmed |
Chemical and Microbial Characterization of North Slope Viscous Oils to Assess Viscosity Reduction and Enhanced Recovery |
title_sort |
chemical and microbial characterization of north slope viscous oils to assess viscosity reduction and enhanced recovery |
publisher |
University of Alaska |
publishDate |
2008 |
url |
https://doi.org/10.2172/963366 https://digital.library.unt.edu/ark:/67531/metadc933863/ |
long_lat |
ENVELOPE(-100.852,-100.852,73.835,73.835) |
geographic |
Milne Point |
geographic_facet |
Milne Point |
genre |
Alaska North Slope north slope Alaska |
genre_facet |
Alaska North Slope north slope Alaska |
op_relation |
grantno: FC26-01NT41248 doi:10.2172/963366 osti: 963366 https://digital.library.unt.edu/ark:/67531/metadc933863/ ark: ark:/67531/metadc933863 |
op_doi |
https://doi.org/10.2172/963366 |
_version_ |
1766167104419004416 |