Effects of Deepwater Horizon Crude Oil on Visual Function in Teleost Fishes
The Deepwater Horizon oil spill released millions of barrels of oil into the Gulf of Mexico, impacting economically and ecologically important fishes. Polycyclic aromatic hydrocarbons (PAHs) present in the oil have been shown to cause developmental impairments in early life stage fishes, such as mor...
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ftunivnotexas:info:ark/67531/metadc1248397 2023-10-09T21:55:35+02:00 Effects of Deepwater Horizon Crude Oil on Visual Function in Teleost Fishes Magnuson, Jason T Roberts, Aaron Venables, Barney Mager, Ed Lund, Amie Morris, Jeff Mexico United States 2018-08 viii, 100 pages Text https://digital.library.unt.edu/ark:/67531/metadc1248397/ English eng University of North Texas local-cont-no: submission_1298 https://digital.library.unt.edu/ark:/67531/metadc1248397/ ark: ark:/67531/metadc1248397 Public Magnuson, Jason T Copyright Copyright is held by the author, unless otherwise noted. All rights Reserved. Visual function Crude oil Teleost fishes Fishes -- Effect of oil spills on -- Mexico Gulf of Polycyclic aromatic hydrocarbons -- Physiological effect -- Mexico Fishes -- Larvae -- Mexico Fishes -- Development -- Mexico Fishes -- Sense organs BP Deepwater Horizon Explosion and Oil Spill 2010 Thesis or Dissertation 2018 ftunivnotexas 2023-09-23T22:07:50Z The Deepwater Horizon oil spill released millions of barrels of oil into the Gulf of Mexico, impacting economically and ecologically important fishes. Polycyclic aromatic hydrocarbons (PAHs) present in the oil have been shown to cause developmental impairments in early life stage fishes, such as morphological and behavioral changes related to eye formation and visual processing following PAH exposure. Prior research reported reduced eye growth in open water, pelagic species, as well as reduced photoreceptor-specific transcription factors associated with eye development following exposure to crude oil. Though changes in transcriptomic-level pathways associated with vision and visual processing have been reported, it has yet to be determined how these changes relate to physiological or behavioral-level effects in fish. Therefore, the present studies evaluated the effect of weathered crude oil on eye development and visual function in mahi-mahi, red drum, sheepshead minnow, and zebrafish larvae. Fish were assessed through several visually-mediated behavioral assays, analyzed histologically and immunohistologically, along with subsequent transcriptomic analyses and associated gene expression changes. Larvae exposed to crude oil experienced significantly reduced abilities to exhibit optomotor or optokinetic responses relative to controls, with associated reductions in retinal development. Furthermore, genes associated with eye development and phototransduction were downregulated, with subsequent decreases in the immunofluorescence of neurological connections within the retina and a choroid-specific increase in apoptotic activity. We related oil-induced transcriptomic-level effects to morphological, physiological, and behavioral-level impairments in larval teleost fishes. Thesis Red drum University of North Texas: UNT Digital Library |
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Open Polar |
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University of North Texas: UNT Digital Library |
op_collection_id |
ftunivnotexas |
language |
English |
topic |
Visual function Crude oil Teleost fishes Fishes -- Effect of oil spills on -- Mexico Gulf of Polycyclic aromatic hydrocarbons -- Physiological effect -- Mexico Fishes -- Larvae -- Mexico Fishes -- Development -- Mexico Fishes -- Sense organs BP Deepwater Horizon Explosion and Oil Spill 2010 |
spellingShingle |
Visual function Crude oil Teleost fishes Fishes -- Effect of oil spills on -- Mexico Gulf of Polycyclic aromatic hydrocarbons -- Physiological effect -- Mexico Fishes -- Larvae -- Mexico Fishes -- Development -- Mexico Fishes -- Sense organs BP Deepwater Horizon Explosion and Oil Spill 2010 Magnuson, Jason T Effects of Deepwater Horizon Crude Oil on Visual Function in Teleost Fishes |
topic_facet |
Visual function Crude oil Teleost fishes Fishes -- Effect of oil spills on -- Mexico Gulf of Polycyclic aromatic hydrocarbons -- Physiological effect -- Mexico Fishes -- Larvae -- Mexico Fishes -- Development -- Mexico Fishes -- Sense organs BP Deepwater Horizon Explosion and Oil Spill 2010 |
description |
The Deepwater Horizon oil spill released millions of barrels of oil into the Gulf of Mexico, impacting economically and ecologically important fishes. Polycyclic aromatic hydrocarbons (PAHs) present in the oil have been shown to cause developmental impairments in early life stage fishes, such as morphological and behavioral changes related to eye formation and visual processing following PAH exposure. Prior research reported reduced eye growth in open water, pelagic species, as well as reduced photoreceptor-specific transcription factors associated with eye development following exposure to crude oil. Though changes in transcriptomic-level pathways associated with vision and visual processing have been reported, it has yet to be determined how these changes relate to physiological or behavioral-level effects in fish. Therefore, the present studies evaluated the effect of weathered crude oil on eye development and visual function in mahi-mahi, red drum, sheepshead minnow, and zebrafish larvae. Fish were assessed through several visually-mediated behavioral assays, analyzed histologically and immunohistologically, along with subsequent transcriptomic analyses and associated gene expression changes. Larvae exposed to crude oil experienced significantly reduced abilities to exhibit optomotor or optokinetic responses relative to controls, with associated reductions in retinal development. Furthermore, genes associated with eye development and phototransduction were downregulated, with subsequent decreases in the immunofluorescence of neurological connections within the retina and a choroid-specific increase in apoptotic activity. We related oil-induced transcriptomic-level effects to morphological, physiological, and behavioral-level impairments in larval teleost fishes. |
author2 |
Roberts, Aaron Venables, Barney Mager, Ed Lund, Amie Morris, Jeff |
format |
Thesis |
author |
Magnuson, Jason T |
author_facet |
Magnuson, Jason T |
author_sort |
Magnuson, Jason T |
title |
Effects of Deepwater Horizon Crude Oil on Visual Function in Teleost Fishes |
title_short |
Effects of Deepwater Horizon Crude Oil on Visual Function in Teleost Fishes |
title_full |
Effects of Deepwater Horizon Crude Oil on Visual Function in Teleost Fishes |
title_fullStr |
Effects of Deepwater Horizon Crude Oil on Visual Function in Teleost Fishes |
title_full_unstemmed |
Effects of Deepwater Horizon Crude Oil on Visual Function in Teleost Fishes |
title_sort |
effects of deepwater horizon crude oil on visual function in teleost fishes |
publisher |
University of North Texas |
publishDate |
2018 |
url |
https://digital.library.unt.edu/ark:/67531/metadc1248397/ |
op_coverage |
Mexico United States |
genre |
Red drum |
genre_facet |
Red drum |
op_relation |
local-cont-no: submission_1298 https://digital.library.unt.edu/ark:/67531/metadc1248397/ ark: ark:/67531/metadc1248397 |
op_rights |
Public Magnuson, Jason T Copyright Copyright is held by the author, unless otherwise noted. All rights Reserved. |
_version_ |
1779319545381519360 |