Establishment and characterization of pygmy killer whale (Feresa attenuata) dermal fibroblast cell line
The pygmy killer whale (Feresa attenuata) (PKW) is a tropical and subtropical marine mammal commonly found in the Atlantic, Indian and Pacific oceans. Since the PKWs live in offshore protected territories, they are rarely seen onshore. Hence, PKW are one of the most poorly understood oceanic species...
Published in: | PLOS ONE |
---|---|
Main Authors: | , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Public Library of Science
2018
|
Subjects: | |
Online Access: | https://nrl.northumbria.ac.uk/id/eprint/48724/ https://doi.org/10.1371/journal.pone.0195128 https://nrl.northumbria.ac.uk/id/eprint/48724/1/file.pdf |
id |
ftunivnorthumb:oai:nrl.northumbria.ac.uk:48724 |
---|---|
record_format |
openpolar |
spelling |
ftunivnorthumb:oai:nrl.northumbria.ac.uk:48724 2023-05-15T17:03:31+02:00 Establishment and characterization of pygmy killer whale (Feresa attenuata) dermal fibroblast cell line Woeller, Collynn Yajing, Sun Rajput, Imran Rashid Ying, Huang Fei, Yu Sanganyado, Edmond Ping, Li Jingzhen, Wang Wenhua, Liu 2018-03-29 text https://nrl.northumbria.ac.uk/id/eprint/48724/ https://doi.org/10.1371/journal.pone.0195128 https://nrl.northumbria.ac.uk/id/eprint/48724/1/file.pdf en eng Public Library of Science https://nrl.northumbria.ac.uk/id/eprint/48724/1/file.pdf Woeller, Collynn, Yajing, Sun, Rajput, Imran Rashid, Ying, Huang, Fei, Yu, Sanganyado, Edmond, Ping, Li, Jingzhen, Wang and Wenhua, Liu (2018) Establishment and characterization of pygmy killer whale (Feresa attenuata) dermal fibroblast cell line. PLoS ONE, 13 (3). e0195128. ISSN 1932-6203 cc_by_4_0 CC-BY C100 Biology C900 Others in Biological Sciences Article PeerReviewed 2018 ftunivnorthumb https://doi.org/10.1371/journal.pone.0195128 2022-09-25T06:15:22Z The pygmy killer whale (Feresa attenuata) (PKW) is a tropical and subtropical marine mammal commonly found in the Atlantic, Indian and Pacific oceans. Since the PKWs live in offshore protected territories, they are rarely seen onshore. Hence, PKW are one of the most poorly understood oceanic species of odontocetes. The dermal tissue comes primarily from stranding events that occur along the coast of the Shantou, Guangdong, China. The sampled tissues were immediately processed and attached on collagen-coated 6-well tissue culture plate. The complete medium (DMEM and Ham’s F12, fetal bovine serum, antibiotic and essential amino acids) was added to the culture plates. The primary culture (PKW-LWH) cells were verified as fibroblast by vimentin and karyotype analyses, which revealed 42 autosomes and two sex chromosomes X and Y. Following transfection of PKW-LWH cells with a plasmid encoding, the SV40 large T-antigens and the transfected cells were isolated and expanded. Using RT-PCR, western blot, immunofluorescence analysis and SV40 large T-antigen stability was confirmed. The cell proliferation rate of the fibroblast cells, PKW-LWHT was faster than the primary cells PKW-LWH with the doubling time 68.9h and 14.4h, respectively. In this study, we established PKW dermal fibroblast cell line for the first time, providing a unique opportunity for in vitro studies on the effects of environmental pollutants and pathogens that could be determined in PKW and/or Cetaceans. Article in Journal/Newspaper Killer Whale Killer whale Northumbria University, Newcastle: Northumbria Research Link (NRL) Indian Pacific PLOS ONE 13 3 e0195128 |
institution |
Open Polar |
collection |
Northumbria University, Newcastle: Northumbria Research Link (NRL) |
op_collection_id |
ftunivnorthumb |
language |
English |
topic |
C100 Biology C900 Others in Biological Sciences |
spellingShingle |
C100 Biology C900 Others in Biological Sciences Woeller, Collynn Yajing, Sun Rajput, Imran Rashid Ying, Huang Fei, Yu Sanganyado, Edmond Ping, Li Jingzhen, Wang Wenhua, Liu Establishment and characterization of pygmy killer whale (Feresa attenuata) dermal fibroblast cell line |
topic_facet |
C100 Biology C900 Others in Biological Sciences |
description |
The pygmy killer whale (Feresa attenuata) (PKW) is a tropical and subtropical marine mammal commonly found in the Atlantic, Indian and Pacific oceans. Since the PKWs live in offshore protected territories, they are rarely seen onshore. Hence, PKW are one of the most poorly understood oceanic species of odontocetes. The dermal tissue comes primarily from stranding events that occur along the coast of the Shantou, Guangdong, China. The sampled tissues were immediately processed and attached on collagen-coated 6-well tissue culture plate. The complete medium (DMEM and Ham’s F12, fetal bovine serum, antibiotic and essential amino acids) was added to the culture plates. The primary culture (PKW-LWH) cells were verified as fibroblast by vimentin and karyotype analyses, which revealed 42 autosomes and two sex chromosomes X and Y. Following transfection of PKW-LWH cells with a plasmid encoding, the SV40 large T-antigens and the transfected cells were isolated and expanded. Using RT-PCR, western blot, immunofluorescence analysis and SV40 large T-antigen stability was confirmed. The cell proliferation rate of the fibroblast cells, PKW-LWHT was faster than the primary cells PKW-LWH with the doubling time 68.9h and 14.4h, respectively. In this study, we established PKW dermal fibroblast cell line for the first time, providing a unique opportunity for in vitro studies on the effects of environmental pollutants and pathogens that could be determined in PKW and/or Cetaceans. |
format |
Article in Journal/Newspaper |
author |
Woeller, Collynn Yajing, Sun Rajput, Imran Rashid Ying, Huang Fei, Yu Sanganyado, Edmond Ping, Li Jingzhen, Wang Wenhua, Liu |
author_facet |
Woeller, Collynn Yajing, Sun Rajput, Imran Rashid Ying, Huang Fei, Yu Sanganyado, Edmond Ping, Li Jingzhen, Wang Wenhua, Liu |
author_sort |
Woeller, Collynn |
title |
Establishment and characterization of pygmy killer whale (Feresa attenuata) dermal fibroblast cell line |
title_short |
Establishment and characterization of pygmy killer whale (Feresa attenuata) dermal fibroblast cell line |
title_full |
Establishment and characterization of pygmy killer whale (Feresa attenuata) dermal fibroblast cell line |
title_fullStr |
Establishment and characterization of pygmy killer whale (Feresa attenuata) dermal fibroblast cell line |
title_full_unstemmed |
Establishment and characterization of pygmy killer whale (Feresa attenuata) dermal fibroblast cell line |
title_sort |
establishment and characterization of pygmy killer whale (feresa attenuata) dermal fibroblast cell line |
publisher |
Public Library of Science |
publishDate |
2018 |
url |
https://nrl.northumbria.ac.uk/id/eprint/48724/ https://doi.org/10.1371/journal.pone.0195128 https://nrl.northumbria.ac.uk/id/eprint/48724/1/file.pdf |
geographic |
Indian Pacific |
geographic_facet |
Indian Pacific |
genre |
Killer Whale Killer whale |
genre_facet |
Killer Whale Killer whale |
op_relation |
https://nrl.northumbria.ac.uk/id/eprint/48724/1/file.pdf Woeller, Collynn, Yajing, Sun, Rajput, Imran Rashid, Ying, Huang, Fei, Yu, Sanganyado, Edmond, Ping, Li, Jingzhen, Wang and Wenhua, Liu (2018) Establishment and characterization of pygmy killer whale (Feresa attenuata) dermal fibroblast cell line. PLoS ONE, 13 (3). e0195128. ISSN 1932-6203 |
op_rights |
cc_by_4_0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1371/journal.pone.0195128 |
container_title |
PLOS ONE |
container_volume |
13 |
container_issue |
3 |
container_start_page |
e0195128 |
_version_ |
1766057406427561984 |