Establishment and characterization of pygmy killer whale (Feresa attenuata) dermal fibroblast cell line

The pygmy killer whale (Feresa attenuata) (PKW) is a tropical and subtropical marine mammal commonly found in the Atlantic, Indian and Pacific oceans. Since the PKWs live in offshore protected territories, they are rarely seen onshore. Hence, PKW are one of the most poorly understood oceanic species...

Full description

Bibliographic Details
Published in:PLOS ONE
Main Authors: Woeller, Collynn, Yajing, Sun, Rajput, Imran Rashid, Ying, Huang, Fei, Yu, Sanganyado, Edmond, Ping, Li, Jingzhen, Wang, Wenhua, Liu
Format: Article in Journal/Newspaper
Language:English
Published: Public Library of Science 2018
Subjects:
C100 Biology
C900 Others in Biological Sciences
Indian
Pacific
Killer Whale
Killer whale
Online Access:https://nrl.northumbria.ac.uk/id/eprint/48724/
https://doi.org/10.1371/journal.pone.0195128
https://nrl.northumbria.ac.uk/id/eprint/48724/1/file.pdf
Description
Summary:The pygmy killer whale (Feresa attenuata) (PKW) is a tropical and subtropical marine mammal commonly found in the Atlantic, Indian and Pacific oceans. Since the PKWs live in offshore protected territories, they are rarely seen onshore. Hence, PKW are one of the most poorly understood oceanic species of odontocetes. The dermal tissue comes primarily from stranding events that occur along the coast of the Shantou, Guangdong, China. The sampled tissues were immediately processed and attached on collagen-coated 6-well tissue culture plate. The complete medium (DMEM and Ham’s F12, fetal bovine serum, antibiotic and essential amino acids) was added to the culture plates. The primary culture (PKW-LWH) cells were verified as fibroblast by vimentin and karyotype analyses, which revealed 42 autosomes and two sex chromosomes X and Y. Following transfection of PKW-LWH cells with a plasmid encoding, the SV40 large T-antigens and the transfected cells were isolated and expanded. Using RT-PCR, western blot, immunofluorescence analysis and SV40 large T-antigen stability was confirmed. The cell proliferation rate of the fibroblast cells, PKW-LWHT was faster than the primary cells PKW-LWH with the doubling time 68.9h and 14.4h, respectively. In this study, we established PKW dermal fibroblast cell line for the first time, providing a unique opportunity for in vitro studies on the effects of environmental pollutants and pathogens that could be determined in PKW and/or Cetaceans.

Similar Items