Isolation, Characterisation, and Lipase Production of a Cold-Adapted Bacterial Strain Pseudomonas sp. LSK25 Isolated from Signy Island, Antarctica
In recent years, studies on psychrophilic lipases have been an emerging area of research in the field of enzymology. This study focuses on bacterial strains isolated from anthropogenically-influenced soil samples collected around Signy Island Research Station (South Orkney Islands, maritime Antarcti...
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ftunivnorthumb:oai:nrl.northumbria.ac.uk:38110 2023-05-15T13:56:54+02:00 Isolation, Characterisation, and Lipase Production of a Cold-Adapted Bacterial Strain Pseudomonas sp. LSK25 Isolated from Signy Island, Antarctica Salwoom, Leelatulasi Raja Abd Rahman, Raja Salleh, Abu Mohd. Shariff, Fairolniza Convey, Peter Pearce, David Mohamad Ali, Mohd 2019-02-16 text https://nrl.northumbria.ac.uk/id/eprint/38110/ https://doi.org/10.3390/molecules24040715 https://nrl.northumbria.ac.uk/id/eprint/38110/1/molecules-24-00715.pdf en eng MDPI https://nrl.northumbria.ac.uk/id/eprint/38110/1/molecules-24-00715.pdf Salwoom, Leelatulasi, Raja Abd Rahman, Raja, Salleh, Abu, Mohd. Shariff, Fairolniza, Convey, Peter, Pearce, David and Mohamad Ali, Mohd (2019) Isolation, Characterisation, and Lipase Production of a Cold-Adapted Bacterial Strain Pseudomonas sp. LSK25 Isolated from Signy Island, Antarctica. Molecules, 24 (4). p. 715. ISSN 1420-3049 cc_by_4_0 CC-BY C700 Molecular Biology Biophysics and Biochemistry Article PeerReviewed 2019 ftunivnorthumb https://doi.org/10.3390/molecules24040715 2022-09-25T06:09:16Z In recent years, studies on psychrophilic lipases have been an emerging area of research in the field of enzymology. This study focuses on bacterial strains isolated from anthropogenically-influenced soil samples collected around Signy Island Research Station (South Orkney Islands, maritime Antarctic). Limited information on lipase activities from bacteria isolated from Signy station is currently available. The presence of lipase genes was determined using real time quantification PCR (qPCR) in samples obtained from three different locations on Signy Island. Twenty strains from the location with highest lipase gene detection were screened for lipolytic activities at a temperature of 4 °C, and from this one strain was selected for further examination based on the highest enzymatic activities obtained. Analysis of 16S rRNA sequence data of this strain showed the highest level of sequence similarity (98%) to a Pseudomonas sp. strain also isolated from Antarctica. In order to increase lipase production of this psychrophilic strain, optimisation of different parameters of physical and nutritional factors were investigated. Optimal production was obtained at 10 °C and pH 7.0, at 150 rev/min shaking rate over 36 h incubation. Article in Journal/Newspaper Antarc* Antarctic Antarctica Signy Island South Orkney Islands Northumbria University, Newcastle: Northumbria Research Link (NRL) Antarctic Signy Island ENVELOPE(-45.595,-45.595,-60.708,-60.708) Signy Station ENVELOPE(-45.600,-45.600,-60.717,-60.717) South Orkney Islands ENVELOPE(-45.500,-45.500,-60.583,-60.583) Molecules 24 4 715 |
institution |
Open Polar |
collection |
Northumbria University, Newcastle: Northumbria Research Link (NRL) |
op_collection_id |
ftunivnorthumb |
language |
English |
topic |
C700 Molecular Biology Biophysics and Biochemistry |
spellingShingle |
C700 Molecular Biology Biophysics and Biochemistry Salwoom, Leelatulasi Raja Abd Rahman, Raja Salleh, Abu Mohd. Shariff, Fairolniza Convey, Peter Pearce, David Mohamad Ali, Mohd Isolation, Characterisation, and Lipase Production of a Cold-Adapted Bacterial Strain Pseudomonas sp. LSK25 Isolated from Signy Island, Antarctica |
topic_facet |
C700 Molecular Biology Biophysics and Biochemistry |
description |
In recent years, studies on psychrophilic lipases have been an emerging area of research in the field of enzymology. This study focuses on bacterial strains isolated from anthropogenically-influenced soil samples collected around Signy Island Research Station (South Orkney Islands, maritime Antarctic). Limited information on lipase activities from bacteria isolated from Signy station is currently available. The presence of lipase genes was determined using real time quantification PCR (qPCR) in samples obtained from three different locations on Signy Island. Twenty strains from the location with highest lipase gene detection were screened for lipolytic activities at a temperature of 4 °C, and from this one strain was selected for further examination based on the highest enzymatic activities obtained. Analysis of 16S rRNA sequence data of this strain showed the highest level of sequence similarity (98%) to a Pseudomonas sp. strain also isolated from Antarctica. In order to increase lipase production of this psychrophilic strain, optimisation of different parameters of physical and nutritional factors were investigated. Optimal production was obtained at 10 °C and pH 7.0, at 150 rev/min shaking rate over 36 h incubation. |
format |
Article in Journal/Newspaper |
author |
Salwoom, Leelatulasi Raja Abd Rahman, Raja Salleh, Abu Mohd. Shariff, Fairolniza Convey, Peter Pearce, David Mohamad Ali, Mohd |
author_facet |
Salwoom, Leelatulasi Raja Abd Rahman, Raja Salleh, Abu Mohd. Shariff, Fairolniza Convey, Peter Pearce, David Mohamad Ali, Mohd |
author_sort |
Salwoom, Leelatulasi |
title |
Isolation, Characterisation, and Lipase Production of a Cold-Adapted Bacterial Strain Pseudomonas sp. LSK25 Isolated from Signy Island, Antarctica |
title_short |
Isolation, Characterisation, and Lipase Production of a Cold-Adapted Bacterial Strain Pseudomonas sp. LSK25 Isolated from Signy Island, Antarctica |
title_full |
Isolation, Characterisation, and Lipase Production of a Cold-Adapted Bacterial Strain Pseudomonas sp. LSK25 Isolated from Signy Island, Antarctica |
title_fullStr |
Isolation, Characterisation, and Lipase Production of a Cold-Adapted Bacterial Strain Pseudomonas sp. LSK25 Isolated from Signy Island, Antarctica |
title_full_unstemmed |
Isolation, Characterisation, and Lipase Production of a Cold-Adapted Bacterial Strain Pseudomonas sp. LSK25 Isolated from Signy Island, Antarctica |
title_sort |
isolation, characterisation, and lipase production of a cold-adapted bacterial strain pseudomonas sp. lsk25 isolated from signy island, antarctica |
publisher |
MDPI |
publishDate |
2019 |
url |
https://nrl.northumbria.ac.uk/id/eprint/38110/ https://doi.org/10.3390/molecules24040715 https://nrl.northumbria.ac.uk/id/eprint/38110/1/molecules-24-00715.pdf |
long_lat |
ENVELOPE(-45.595,-45.595,-60.708,-60.708) ENVELOPE(-45.600,-45.600,-60.717,-60.717) ENVELOPE(-45.500,-45.500,-60.583,-60.583) |
geographic |
Antarctic Signy Island Signy Station South Orkney Islands |
geographic_facet |
Antarctic Signy Island Signy Station South Orkney Islands |
genre |
Antarc* Antarctic Antarctica Signy Island South Orkney Islands |
genre_facet |
Antarc* Antarctic Antarctica Signy Island South Orkney Islands |
op_relation |
https://nrl.northumbria.ac.uk/id/eprint/38110/1/molecules-24-00715.pdf Salwoom, Leelatulasi, Raja Abd Rahman, Raja, Salleh, Abu, Mohd. Shariff, Fairolniza, Convey, Peter, Pearce, David and Mohamad Ali, Mohd (2019) Isolation, Characterisation, and Lipase Production of a Cold-Adapted Bacterial Strain Pseudomonas sp. LSK25 Isolated from Signy Island, Antarctica. Molecules, 24 (4). p. 715. ISSN 1420-3049 |
op_rights |
cc_by_4_0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3390/molecules24040715 |
container_title |
Molecules |
container_volume |
24 |
container_issue |
4 |
container_start_page |
715 |
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1766264504359845888 |