Bacterial communities associated with the Southern Ocean vent gastropod, Gigantopelta chessoia: indication of horizontal symbiont transfer
Recently discovered hydrothermal vents of the East Scotia Ridge (ESR) in the Southern Ocean host unique faunal communities that depend on microbial chemosynthetic primary production. These highly abundant invertebrates gain energy from either grazing on free-living microbes or via hosting symbiotic...
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ftunivnorthumb:oai:nrl.northumbria.ac.uk:31407 2023-05-15T13:56:54+02:00 Bacterial communities associated with the Southern Ocean vent gastropod, Gigantopelta chessoia: indication of horizontal symbiont transfer Heywood, Jane Chen, Chong Pearce, David Linse, Katrin 2017-11 https://nrl.northumbria.ac.uk/id/eprint/31407/ https://doi.org/10.1007/s00300-017-2148-6 unknown Springer Heywood, Jane, Chen, Chong, Pearce, David and Linse, Katrin (2017) Bacterial communities associated with the Southern Ocean vent gastropod, Gigantopelta chessoia: indication of horizontal symbiont transfer. Polar Biology, 40 (11). pp. 2335-2342. ISSN 0722-4060 C300 Zoology C500 Microbiology Article PeerReviewed 2017 ftunivnorthumb https://doi.org/10.1007/s00300-017-2148-6 2022-09-25T06:05:54Z Recently discovered hydrothermal vents of the East Scotia Ridge (ESR) in the Southern Ocean host unique faunal communities that depend on microbial chemosynthetic primary production. These highly abundant invertebrates gain energy from either grazing on free-living microbes or via hosting symbiotic chemoautotrophic microorganisms. The main objective of this study was to characterise microbes associated with a newly discovered species of hydrothermal vent gastropod and therefore increase knowledge of ecosystem functioning in this largely unknown Antarctic hydrothermal vent system. We investigated the phylogenetic composition of bacteria associated with the gills and oesophageal gland of the ESR peltospirid gastropod, Gigantopelta chessoia by molecular cloning and terminal restriction fragment length polymorphism (T-RFLP). 16S rRNA gene clone libraries revealed host tissue-specific combinations of bacteria. The oesophageal gland contained one Gammaproteobacteria OTU whereas a more diverse community of Gamma, Epsilon and Deltaproteobacteria was isolated from the gills. T-RFLP analysis revealed that juvenile bacterial communities were more closely related to adult gill-associated bacterial communities than oesophageal gland bacteria. Oesophageal gland Gammaproteobacteria exhibited a higher sequence similarity with sulphur-oxidising bacteria isolated from cold seep sediments and with thioautotrophic endosymbionts than with bacteria found in the surrounding water column, suggesting that these endosymbionts were not acquired directly from the water column. Juvenile G. chessoia were located within the mantle cavity of adults and we speculate that Gammaproteobacterial endosymbionts in the oesophageal gland could be transmitted horizontally from adults to juveniles via the gills due to the close contact of juveniles with adults' gills. Article in Journal/Newspaper Antarc* Antarctic Polar Biology Southern Ocean Northumbria University, Newcastle: Northumbria Research Link (NRL) Antarctic East Scotia Ridge ENVELOPE(-29.250,-29.250,-57.917,-57.917) Southern Ocean Polar Biology 40 11 2335 2342 |
institution |
Open Polar |
collection |
Northumbria University, Newcastle: Northumbria Research Link (NRL) |
op_collection_id |
ftunivnorthumb |
language |
unknown |
topic |
C300 Zoology C500 Microbiology |
spellingShingle |
C300 Zoology C500 Microbiology Heywood, Jane Chen, Chong Pearce, David Linse, Katrin Bacterial communities associated with the Southern Ocean vent gastropod, Gigantopelta chessoia: indication of horizontal symbiont transfer |
topic_facet |
C300 Zoology C500 Microbiology |
description |
Recently discovered hydrothermal vents of the East Scotia Ridge (ESR) in the Southern Ocean host unique faunal communities that depend on microbial chemosynthetic primary production. These highly abundant invertebrates gain energy from either grazing on free-living microbes or via hosting symbiotic chemoautotrophic microorganisms. The main objective of this study was to characterise microbes associated with a newly discovered species of hydrothermal vent gastropod and therefore increase knowledge of ecosystem functioning in this largely unknown Antarctic hydrothermal vent system. We investigated the phylogenetic composition of bacteria associated with the gills and oesophageal gland of the ESR peltospirid gastropod, Gigantopelta chessoia by molecular cloning and terminal restriction fragment length polymorphism (T-RFLP). 16S rRNA gene clone libraries revealed host tissue-specific combinations of bacteria. The oesophageal gland contained one Gammaproteobacteria OTU whereas a more diverse community of Gamma, Epsilon and Deltaproteobacteria was isolated from the gills. T-RFLP analysis revealed that juvenile bacterial communities were more closely related to adult gill-associated bacterial communities than oesophageal gland bacteria. Oesophageal gland Gammaproteobacteria exhibited a higher sequence similarity with sulphur-oxidising bacteria isolated from cold seep sediments and with thioautotrophic endosymbionts than with bacteria found in the surrounding water column, suggesting that these endosymbionts were not acquired directly from the water column. Juvenile G. chessoia were located within the mantle cavity of adults and we speculate that Gammaproteobacterial endosymbionts in the oesophageal gland could be transmitted horizontally from adults to juveniles via the gills due to the close contact of juveniles with adults' gills. |
format |
Article in Journal/Newspaper |
author |
Heywood, Jane Chen, Chong Pearce, David Linse, Katrin |
author_facet |
Heywood, Jane Chen, Chong Pearce, David Linse, Katrin |
author_sort |
Heywood, Jane |
title |
Bacterial communities associated with the Southern Ocean vent gastropod, Gigantopelta chessoia: indication of horizontal symbiont transfer |
title_short |
Bacterial communities associated with the Southern Ocean vent gastropod, Gigantopelta chessoia: indication of horizontal symbiont transfer |
title_full |
Bacterial communities associated with the Southern Ocean vent gastropod, Gigantopelta chessoia: indication of horizontal symbiont transfer |
title_fullStr |
Bacterial communities associated with the Southern Ocean vent gastropod, Gigantopelta chessoia: indication of horizontal symbiont transfer |
title_full_unstemmed |
Bacterial communities associated with the Southern Ocean vent gastropod, Gigantopelta chessoia: indication of horizontal symbiont transfer |
title_sort |
bacterial communities associated with the southern ocean vent gastropod, gigantopelta chessoia: indication of horizontal symbiont transfer |
publisher |
Springer |
publishDate |
2017 |
url |
https://nrl.northumbria.ac.uk/id/eprint/31407/ https://doi.org/10.1007/s00300-017-2148-6 |
long_lat |
ENVELOPE(-29.250,-29.250,-57.917,-57.917) |
geographic |
Antarctic East Scotia Ridge Southern Ocean |
geographic_facet |
Antarctic East Scotia Ridge Southern Ocean |
genre |
Antarc* Antarctic Polar Biology Southern Ocean |
genre_facet |
Antarc* Antarctic Polar Biology Southern Ocean |
op_relation |
Heywood, Jane, Chen, Chong, Pearce, David and Linse, Katrin (2017) Bacterial communities associated with the Southern Ocean vent gastropod, Gigantopelta chessoia: indication of horizontal symbiont transfer. Polar Biology, 40 (11). pp. 2335-2342. ISSN 0722-4060 |
op_doi |
https://doi.org/10.1007/s00300-017-2148-6 |
container_title |
Polar Biology |
container_volume |
40 |
container_issue |
11 |
container_start_page |
2335 |
op_container_end_page |
2342 |
_version_ |
1766264489061122048 |