Planococcus versutus sp. nov., isolated from soil
A taxonomic study was performed on a novel Gram-stain-positive, coccus-shaped, orange-pigmented motile bacterium, designated as strain L10.15T. The organism was isolated from a soil sample collected in Lagoon Island (close to Adelaide Island, western Antarctic Peninsula) using a quorum-quenching enr...
Published in: | International Journal of Systematic and Evolutionary Microbiology |
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Language: | English |
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Society for General Microbiology
2017
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Online Access: | https://nrl.northumbria.ac.uk/id/eprint/30914/ https://doi.org/10.1099/ijsem.0.001721 https://nrl.northumbria.ac.uk/id/eprint/30914/1/IJSEM-D-16-00692_R2-2-2.pdf |
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ftunivnorthumb:oai:nrl.northumbria.ac.uk:30914 2023-05-15T13:03:58+02:00 Planococcus versutus sp. nov., isolated from soil See-Too, Wah-Seng Ee, Robson Madhaiyan, Munusamy Kwon, Soon-Wo Tan, Jia Yi Lim, Yan Lue Convey, Peter Pearce, David Yin, Wai Fong Chan, Kok-Gan 2017-04-01 text https://nrl.northumbria.ac.uk/id/eprint/30914/ https://doi.org/10.1099/ijsem.0.001721 https://nrl.northumbria.ac.uk/id/eprint/30914/1/IJSEM-D-16-00692_R2-2-2.pdf en eng Society for General Microbiology https://nrl.northumbria.ac.uk/id/eprint/30914/1/IJSEM-D-16-00692_R2-2-2.pdf See-Too, Wah-Seng, Ee, Robson, Madhaiyan, Munusamy, Kwon, Soon-Wo, Tan, Jia Yi, Lim, Yan Lue, Convey, Peter, Pearce, David, Yin, Wai Fong and Chan, Kok-Gan (2017) Planococcus versutus sp. nov., isolated from soil. International Journal of Systematic and Evolutionary Microbiology, 67 (4). pp. 944-950. ISSN 1466-5026 C500 Microbiology Article PeerReviewed 2017 ftunivnorthumb https://doi.org/10.1099/ijsem.0.001721 2022-09-25T06:05:39Z A taxonomic study was performed on a novel Gram-stain-positive, coccus-shaped, orange-pigmented motile bacterium, designated as strain L10.15T. The organism was isolated from a soil sample collected in Lagoon Island (close to Adelaide Island, western Antarctic Peninsula) using a quorum-quenching enrichment medium. Growth occurred at 4–30 °C, pH 6–11 and at moderately high salinity (0–15 %, w/v, NaCl), with optimal growth at 26 °C, at pH 7–8 and with 6 % (w/v) NaCl. 16S rRNA gene sequence analysis showed that strain L10.15T belonged to the genus Planococcus and was closely related to Planococcus halocryophilus Or1T (99.3 % similarity), Planococcus donghaensis JH1T (99.0 %), Planococcus antarcticus DSM 14505T (98.3 %), Planococcus plakortidis AS/ASP6 (II)T (97.6 %), Planococcus maritimus TF-9T (97.5 %), Planococcus salinarum ISL-6T (97.5 %) and Planococcus kocurii NCIMB 629T (97.5 %). However, the average nucleotide identity-MUMmer analysis showed low genomic relatedness values of 71.1–81.7 % to the type strains of these closely related species of the genus Planococcus . The principal fatty acids were anteiso-C15 : 0, C16 : 1ω7c and anteiso-C17 : 0, and the major menaquinones of strain L10.15T were MK-5 (48 %), MK-6 (6 %) and MK-7 (44 %). Polar lipid analysis revealed the presence of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and aminophospholipid. The DNA G+C content was 39.4 mol%. The phenotypic and genotypic data indicate that strain L10.15T represents a novel species of the genus Planococcus , for which the name Planococcus versutus sp. nov. is proposed. The type strain is L10.15T (=DSM 101994T=KACC 18918T). Article in Journal/Newspaper Adelaide Island Antarc* Antarctic Antarctic Peninsula antarcticus Lagoon Island Northumbria University, Newcastle: Northumbria Research Link (NRL) Adelaide Island ENVELOPE(-68.914,-68.914,-67.762,-67.762) Antarctic Antarctic Peninsula Lagoon Island ENVELOPE(-68.239,-68.239,-67.594,-67.594) International Journal of Systematic and Evolutionary Microbiology 67 4 944 950 |
institution |
Open Polar |
collection |
Northumbria University, Newcastle: Northumbria Research Link (NRL) |
op_collection_id |
ftunivnorthumb |
language |
English |
topic |
C500 Microbiology |
spellingShingle |
C500 Microbiology See-Too, Wah-Seng Ee, Robson Madhaiyan, Munusamy Kwon, Soon-Wo Tan, Jia Yi Lim, Yan Lue Convey, Peter Pearce, David Yin, Wai Fong Chan, Kok-Gan Planococcus versutus sp. nov., isolated from soil |
topic_facet |
C500 Microbiology |
description |
A taxonomic study was performed on a novel Gram-stain-positive, coccus-shaped, orange-pigmented motile bacterium, designated as strain L10.15T. The organism was isolated from a soil sample collected in Lagoon Island (close to Adelaide Island, western Antarctic Peninsula) using a quorum-quenching enrichment medium. Growth occurred at 4–30 °C, pH 6–11 and at moderately high salinity (0–15 %, w/v, NaCl), with optimal growth at 26 °C, at pH 7–8 and with 6 % (w/v) NaCl. 16S rRNA gene sequence analysis showed that strain L10.15T belonged to the genus Planococcus and was closely related to Planococcus halocryophilus Or1T (99.3 % similarity), Planococcus donghaensis JH1T (99.0 %), Planococcus antarcticus DSM 14505T (98.3 %), Planococcus plakortidis AS/ASP6 (II)T (97.6 %), Planococcus maritimus TF-9T (97.5 %), Planococcus salinarum ISL-6T (97.5 %) and Planococcus kocurii NCIMB 629T (97.5 %). However, the average nucleotide identity-MUMmer analysis showed low genomic relatedness values of 71.1–81.7 % to the type strains of these closely related species of the genus Planococcus . The principal fatty acids were anteiso-C15 : 0, C16 : 1ω7c and anteiso-C17 : 0, and the major menaquinones of strain L10.15T were MK-5 (48 %), MK-6 (6 %) and MK-7 (44 %). Polar lipid analysis revealed the presence of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and aminophospholipid. The DNA G+C content was 39.4 mol%. The phenotypic and genotypic data indicate that strain L10.15T represents a novel species of the genus Planococcus , for which the name Planococcus versutus sp. nov. is proposed. The type strain is L10.15T (=DSM 101994T=KACC 18918T). |
format |
Article in Journal/Newspaper |
author |
See-Too, Wah-Seng Ee, Robson Madhaiyan, Munusamy Kwon, Soon-Wo Tan, Jia Yi Lim, Yan Lue Convey, Peter Pearce, David Yin, Wai Fong Chan, Kok-Gan |
author_facet |
See-Too, Wah-Seng Ee, Robson Madhaiyan, Munusamy Kwon, Soon-Wo Tan, Jia Yi Lim, Yan Lue Convey, Peter Pearce, David Yin, Wai Fong Chan, Kok-Gan |
author_sort |
See-Too, Wah-Seng |
title |
Planococcus versutus sp. nov., isolated from soil |
title_short |
Planococcus versutus sp. nov., isolated from soil |
title_full |
Planococcus versutus sp. nov., isolated from soil |
title_fullStr |
Planococcus versutus sp. nov., isolated from soil |
title_full_unstemmed |
Planococcus versutus sp. nov., isolated from soil |
title_sort |
planococcus versutus sp. nov., isolated from soil |
publisher |
Society for General Microbiology |
publishDate |
2017 |
url |
https://nrl.northumbria.ac.uk/id/eprint/30914/ https://doi.org/10.1099/ijsem.0.001721 https://nrl.northumbria.ac.uk/id/eprint/30914/1/IJSEM-D-16-00692_R2-2-2.pdf |
long_lat |
ENVELOPE(-68.914,-68.914,-67.762,-67.762) ENVELOPE(-68.239,-68.239,-67.594,-67.594) |
geographic |
Adelaide Island Antarctic Antarctic Peninsula Lagoon Island |
geographic_facet |
Adelaide Island Antarctic Antarctic Peninsula Lagoon Island |
genre |
Adelaide Island Antarc* Antarctic Antarctic Peninsula antarcticus Lagoon Island |
genre_facet |
Adelaide Island Antarc* Antarctic Antarctic Peninsula antarcticus Lagoon Island |
op_relation |
https://nrl.northumbria.ac.uk/id/eprint/30914/1/IJSEM-D-16-00692_R2-2-2.pdf See-Too, Wah-Seng, Ee, Robson, Madhaiyan, Munusamy, Kwon, Soon-Wo, Tan, Jia Yi, Lim, Yan Lue, Convey, Peter, Pearce, David, Yin, Wai Fong and Chan, Kok-Gan (2017) Planococcus versutus sp. nov., isolated from soil. International Journal of Systematic and Evolutionary Microbiology, 67 (4). pp. 944-950. ISSN 1466-5026 |
op_doi |
https://doi.org/10.1099/ijsem.0.001721 |
container_title |
International Journal of Systematic and Evolutionary Microbiology |
container_volume |
67 |
container_issue |
4 |
container_start_page |
944 |
op_container_end_page |
950 |
_version_ |
1766349365505425408 |