AidP, a novel N-Acyl homoserine lactonase gene from Antarctic Planococcus sp.
Planococcus is a Gram-positive halotolerant bacterial genus in the phylum Firmicutes, commonly found in various habitats in Antarctica. Quorum quenching (QQ) is the disruption of bacterial cell-to-cell communication (known as quorum sensing), which has previously been described in mesophilic bacteri...
Published in: | Scientific Reports |
---|---|
Main Authors: | , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Nature Publishing
2017
|
Subjects: | |
Online Access: | https://nrl.northumbria.ac.uk/id/eprint/30081/ https://doi.org/10.1038/srep42968 https://nrl.northumbria.ac.uk/id/eprint/30081/1/srep42968.pdf |
id |
ftunivnorthumb:oai:nrl.northumbria.ac.uk:30081 |
---|---|
record_format |
openpolar |
spelling |
ftunivnorthumb:oai:nrl.northumbria.ac.uk:30081 2023-05-15T13:56:54+02:00 AidP, a novel N-Acyl homoserine lactonase gene from Antarctic Planococcus sp. See-Too, Wah Seng Ee, Robson Lim, Yan-Lue Convey, Peter Pearce, David Yin, Wai-Fong Chan, Kok-Gan 2017-02-22 text https://nrl.northumbria.ac.uk/id/eprint/30081/ https://doi.org/10.1038/srep42968 https://nrl.northumbria.ac.uk/id/eprint/30081/1/srep42968.pdf en eng Nature Publishing https://nrl.northumbria.ac.uk/id/eprint/30081/1/srep42968.pdf See-Too, Wah Seng, Ee, Robson, Lim, Yan-Lue, Convey, Peter, Pearce, David, Yin, Wai-Fong and Chan, Kok-Gan (2017) AidP, a novel N-Acyl homoserine lactonase gene from Antarctic Planococcus sp. Scientific Reports, 7. p. 42968. ISSN 2045-2322 cc_by_4_0 CC-BY C500 Microbiology Article PeerReviewed 2017 ftunivnorthumb https://doi.org/10.1038/srep42968 2022-09-25T06:05:19Z Planococcus is a Gram-positive halotolerant bacterial genus in the phylum Firmicutes, commonly found in various habitats in Antarctica. Quorum quenching (QQ) is the disruption of bacterial cell-to-cell communication (known as quorum sensing), which has previously been described in mesophilic bacteria. This study demonstrated the QQ activity of a psychrotolerant strain, Planococcus versutus strain L10.15T, isolated from a soil sample obtained near an elephant seal wallow in Antarctica. Whole genome analysis of this bacterial strain revealed the presence of an N-acyl homoserine lactonase, an enzyme that hydrolyzes the ester bond of the homoserine lactone of N-acyl homoserine lactone (AHLs). Heterologous gene expression in E. coli confirmed its functions for hydrolysis of AHLs, and the gene was designated as aidP (autoinducer degrading gene from Planococcus sp.). The low temperature activity of this enzyme suggested that it is a novel and uncharacterized class of AHL lactonase. This study is the first report on QQ activity of bacteria isolated from the polar regions. Article in Journal/Newspaper Antarc* Antarctic Antarctica Elephant Seal Northumbria University, Newcastle: Northumbria Research Link (NRL) Antarctic Scientific Reports 7 1 |
institution |
Open Polar |
collection |
Northumbria University, Newcastle: Northumbria Research Link (NRL) |
op_collection_id |
ftunivnorthumb |
language |
English |
topic |
C500 Microbiology |
spellingShingle |
C500 Microbiology See-Too, Wah Seng Ee, Robson Lim, Yan-Lue Convey, Peter Pearce, David Yin, Wai-Fong Chan, Kok-Gan AidP, a novel N-Acyl homoserine lactonase gene from Antarctic Planococcus sp. |
topic_facet |
C500 Microbiology |
description |
Planococcus is a Gram-positive halotolerant bacterial genus in the phylum Firmicutes, commonly found in various habitats in Antarctica. Quorum quenching (QQ) is the disruption of bacterial cell-to-cell communication (known as quorum sensing), which has previously been described in mesophilic bacteria. This study demonstrated the QQ activity of a psychrotolerant strain, Planococcus versutus strain L10.15T, isolated from a soil sample obtained near an elephant seal wallow in Antarctica. Whole genome analysis of this bacterial strain revealed the presence of an N-acyl homoserine lactonase, an enzyme that hydrolyzes the ester bond of the homoserine lactone of N-acyl homoserine lactone (AHLs). Heterologous gene expression in E. coli confirmed its functions for hydrolysis of AHLs, and the gene was designated as aidP (autoinducer degrading gene from Planococcus sp.). The low temperature activity of this enzyme suggested that it is a novel and uncharacterized class of AHL lactonase. This study is the first report on QQ activity of bacteria isolated from the polar regions. |
format |
Article in Journal/Newspaper |
author |
See-Too, Wah Seng Ee, Robson Lim, Yan-Lue Convey, Peter Pearce, David Yin, Wai-Fong Chan, Kok-Gan |
author_facet |
See-Too, Wah Seng Ee, Robson Lim, Yan-Lue Convey, Peter Pearce, David Yin, Wai-Fong Chan, Kok-Gan |
author_sort |
See-Too, Wah Seng |
title |
AidP, a novel N-Acyl homoserine lactonase gene from Antarctic Planococcus sp. |
title_short |
AidP, a novel N-Acyl homoserine lactonase gene from Antarctic Planococcus sp. |
title_full |
AidP, a novel N-Acyl homoserine lactonase gene from Antarctic Planococcus sp. |
title_fullStr |
AidP, a novel N-Acyl homoserine lactonase gene from Antarctic Planococcus sp. |
title_full_unstemmed |
AidP, a novel N-Acyl homoserine lactonase gene from Antarctic Planococcus sp. |
title_sort |
aidp, a novel n-acyl homoserine lactonase gene from antarctic planococcus sp. |
publisher |
Nature Publishing |
publishDate |
2017 |
url |
https://nrl.northumbria.ac.uk/id/eprint/30081/ https://doi.org/10.1038/srep42968 https://nrl.northumbria.ac.uk/id/eprint/30081/1/srep42968.pdf |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctica Elephant Seal |
genre_facet |
Antarc* Antarctic Antarctica Elephant Seal |
op_relation |
https://nrl.northumbria.ac.uk/id/eprint/30081/1/srep42968.pdf See-Too, Wah Seng, Ee, Robson, Lim, Yan-Lue, Convey, Peter, Pearce, David, Yin, Wai-Fong and Chan, Kok-Gan (2017) AidP, a novel N-Acyl homoserine lactonase gene from Antarctic Planococcus sp. Scientific Reports, 7. p. 42968. ISSN 2045-2322 |
op_rights |
cc_by_4_0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1038/srep42968 |
container_title |
Scientific Reports |
container_volume |
7 |
container_issue |
1 |
_version_ |
1766264487975845888 |