A method to study bacterioplankton community structure in Antarctic lakes
A method to study bacterioplankton community structure in Antarctic freshwater lake samples is described. Small samples (between 300 and 1000 ml) taken in remote field locations were used for crude DNA extraction, followed by PCR amplification of 16S rRNA gene fragments using group-specific primers....
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Online Access: | https://nrl.northumbria.ac.uk/id/eprint/25063/ https://doi.org/10.1007/s003000050455 |
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ftunivnorthumb:oai:nrl.northumbria.ac.uk:25063 2023-05-15T13:35:33+02:00 A method to study bacterioplankton community structure in Antarctic lakes Pearce, David 2000-04 https://nrl.northumbria.ac.uk/id/eprint/25063/ https://doi.org/10.1007/s003000050455 unknown Springer Pearce, David (2000) A method to study bacterioplankton community structure in Antarctic lakes. Polar Biology, 23 (5). pp. 352-356. ISSN 0722-4060 C500 Microbiology Article PeerReviewed 2000 ftunivnorthumb https://doi.org/10.1007/s003000050455 2022-09-25T06:03:05Z A method to study bacterioplankton community structure in Antarctic freshwater lake samples is described. Small samples (between 300 and 1000 ml) taken in remote field locations were used for crude DNA extraction, followed by PCR amplification of 16S rRNA gene fragments using group-specific primers. The amplification products of the PCR reaction were then separated using denaturing gradient gel electrophoresis to produce a profile of the bacterioplankton community. Whilst the technique is only semi-quantitative, it readily differentiated communities from lakes of different trophic status and from vertical profiles within different lake types. The method offers a sensitive tool for screening and monitoring Antarctic freshwater environments as a precursor and adjunct to more detailed studies. Article in Journal/Newspaper Antarc* Antarctic Polar Biology Northumbria University, Newcastle: Northumbria Research Link (NRL) Antarctic Polar Biology 23 5 352 356 |
institution |
Open Polar |
collection |
Northumbria University, Newcastle: Northumbria Research Link (NRL) |
op_collection_id |
ftunivnorthumb |
language |
unknown |
topic |
C500 Microbiology |
spellingShingle |
C500 Microbiology Pearce, David A method to study bacterioplankton community structure in Antarctic lakes |
topic_facet |
C500 Microbiology |
description |
A method to study bacterioplankton community structure in Antarctic freshwater lake samples is described. Small samples (between 300 and 1000 ml) taken in remote field locations were used for crude DNA extraction, followed by PCR amplification of 16S rRNA gene fragments using group-specific primers. The amplification products of the PCR reaction were then separated using denaturing gradient gel electrophoresis to produce a profile of the bacterioplankton community. Whilst the technique is only semi-quantitative, it readily differentiated communities from lakes of different trophic status and from vertical profiles within different lake types. The method offers a sensitive tool for screening and monitoring Antarctic freshwater environments as a precursor and adjunct to more detailed studies. |
format |
Article in Journal/Newspaper |
author |
Pearce, David |
author_facet |
Pearce, David |
author_sort |
Pearce, David |
title |
A method to study bacterioplankton community structure in Antarctic lakes |
title_short |
A method to study bacterioplankton community structure in Antarctic lakes |
title_full |
A method to study bacterioplankton community structure in Antarctic lakes |
title_fullStr |
A method to study bacterioplankton community structure in Antarctic lakes |
title_full_unstemmed |
A method to study bacterioplankton community structure in Antarctic lakes |
title_sort |
method to study bacterioplankton community structure in antarctic lakes |
publisher |
Springer |
publishDate |
2000 |
url |
https://nrl.northumbria.ac.uk/id/eprint/25063/ https://doi.org/10.1007/s003000050455 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Polar Biology |
genre_facet |
Antarc* Antarctic Polar Biology |
op_relation |
Pearce, David (2000) A method to study bacterioplankton community structure in Antarctic lakes. Polar Biology, 23 (5). pp. 352-356. ISSN 0722-4060 |
op_doi |
https://doi.org/10.1007/s003000050455 |
container_title |
Polar Biology |
container_volume |
23 |
container_issue |
5 |
container_start_page |
352 |
op_container_end_page |
356 |
_version_ |
1766067108928552960 |