Diversity of toxin and non-toxin containing cyanobacterial mats of meltwater ponds on the Antarctic Peninsula: a pyrosequencing approach
Despite their pivotal role as primary producers, there is little information as to the diversity and physiology of cyanobacteria in the meltwater ecosystems of polar regions. Thirty cyanobacterial mats from Adelaide Island, Antarctica were investigated using 16S rRNA gene pyrosequencing and automate...
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ftunivnorthumb:oai:nrl.northumbria.ac.uk:16958 2023-05-15T13:03:57+02:00 Diversity of toxin and non-toxin containing cyanobacterial mats of meltwater ponds on the Antarctic Peninsula: a pyrosequencing approach Kleinteich, Julia Hildebrand, Falk Wood, Susie Cirés, Samuel Agha, Ramsy Quesada, Antonio Pearce, David Convey, Peter Küpper, Frithjof Dietrich, Daniel 2014-10 application/pdf https://nrl.northumbria.ac.uk/id/eprint/16958/ https://doi.org/10.1017/S0954102014000145 https://nrl.northumbria.ac.uk/id/eprint/16958/1/-ANS-S0954102014000145a.pdf en eng Cambridge University Press https://nrl.northumbria.ac.uk/id/eprint/16958/1/-ANS-S0954102014000145a.pdf Kleinteich, Julia, Hildebrand, Falk, Wood, Susie, Cirés, Samuel, Agha, Ramsy, Quesada, Antonio, Pearce, David, Convey, Peter, Küpper, Frithjof and Dietrich, Daniel (2014) Diversity of toxin and non-toxin containing cyanobacterial mats of meltwater ponds on the Antarctic Peninsula: a pyrosequencing approach. Antarctic Science, 26 (5). pp. 521-532. ISSN 0954-1020 C500 Microbiology F800 Physical and Terrestrial Geographical and Environmental Sciences Article PeerReviewed 2014 ftunivnorthumb https://doi.org/10.1017/S0954102014000145 2022-09-25T05:59:39Z Despite their pivotal role as primary producers, there is little information as to the diversity and physiology of cyanobacteria in the meltwater ecosystems of polar regions. Thirty cyanobacterial mats from Adelaide Island, Antarctica were investigated using 16S rRNA gene pyrosequencing and automated ribosomal intergenic spacer analysis, and screened for cyanobacterial toxins using molecular and chemical approaches. A total of 274 operational taxonomic units (OTUs) were detected. The richness ranged between 8 and 33 cyanobacterial OTUs per sample, reflecting a high mat diversity. Leptolyngbya and Phormidium (c. 55% and 37% of the OTUs per mat) were dominant. Cyanobacterial community composition was similar between mats, particularly those obtained from closely adjacent locations. The cyanotoxin microcystin was detected in 26 of 27 mats (10–300 ng g-1 organic mass), while cylindrospermopsin, detected for the first time in Antarctica, was present in 21 of 30 mats (2–156 ng g-1 organic mass). The latter was confirmed via liquid chromatography-mass spectrometry and by the presence of the cyrAB and cyrJ genes. This study demonstrates the usefulness of pyrosequencing for characterizing diverse cyanobacterial communities, and confirms that cyanobacteria from extreme environments produce a similar range of cyanotoxins as their temperate counterparts. Article in Journal/Newspaper Adelaide Island Antarc* Antarctic Antarctic Peninsula Antarctic Science Antarctica Northumbria University, Newcastle: Northumbria Research Link (NRL) Adelaide Island ENVELOPE(-68.914,-68.914,-67.762,-67.762) Antarctic Antarctic Peninsula The Antarctic Antarctic Science 26 5 521 532 |
institution |
Open Polar |
collection |
Northumbria University, Newcastle: Northumbria Research Link (NRL) |
op_collection_id |
ftunivnorthumb |
language |
English |
topic |
C500 Microbiology F800 Physical and Terrestrial Geographical and Environmental Sciences |
spellingShingle |
C500 Microbiology F800 Physical and Terrestrial Geographical and Environmental Sciences Kleinteich, Julia Hildebrand, Falk Wood, Susie Cirés, Samuel Agha, Ramsy Quesada, Antonio Pearce, David Convey, Peter Küpper, Frithjof Dietrich, Daniel Diversity of toxin and non-toxin containing cyanobacterial mats of meltwater ponds on the Antarctic Peninsula: a pyrosequencing approach |
topic_facet |
C500 Microbiology F800 Physical and Terrestrial Geographical and Environmental Sciences |
description |
Despite their pivotal role as primary producers, there is little information as to the diversity and physiology of cyanobacteria in the meltwater ecosystems of polar regions. Thirty cyanobacterial mats from Adelaide Island, Antarctica were investigated using 16S rRNA gene pyrosequencing and automated ribosomal intergenic spacer analysis, and screened for cyanobacterial toxins using molecular and chemical approaches. A total of 274 operational taxonomic units (OTUs) were detected. The richness ranged between 8 and 33 cyanobacterial OTUs per sample, reflecting a high mat diversity. Leptolyngbya and Phormidium (c. 55% and 37% of the OTUs per mat) were dominant. Cyanobacterial community composition was similar between mats, particularly those obtained from closely adjacent locations. The cyanotoxin microcystin was detected in 26 of 27 mats (10–300 ng g-1 organic mass), while cylindrospermopsin, detected for the first time in Antarctica, was present in 21 of 30 mats (2–156 ng g-1 organic mass). The latter was confirmed via liquid chromatography-mass spectrometry and by the presence of the cyrAB and cyrJ genes. This study demonstrates the usefulness of pyrosequencing for characterizing diverse cyanobacterial communities, and confirms that cyanobacteria from extreme environments produce a similar range of cyanotoxins as their temperate counterparts. |
format |
Article in Journal/Newspaper |
author |
Kleinteich, Julia Hildebrand, Falk Wood, Susie Cirés, Samuel Agha, Ramsy Quesada, Antonio Pearce, David Convey, Peter Küpper, Frithjof Dietrich, Daniel |
author_facet |
Kleinteich, Julia Hildebrand, Falk Wood, Susie Cirés, Samuel Agha, Ramsy Quesada, Antonio Pearce, David Convey, Peter Küpper, Frithjof Dietrich, Daniel |
author_sort |
Kleinteich, Julia |
title |
Diversity of toxin and non-toxin containing cyanobacterial mats of meltwater ponds on the Antarctic Peninsula: a pyrosequencing approach |
title_short |
Diversity of toxin and non-toxin containing cyanobacterial mats of meltwater ponds on the Antarctic Peninsula: a pyrosequencing approach |
title_full |
Diversity of toxin and non-toxin containing cyanobacterial mats of meltwater ponds on the Antarctic Peninsula: a pyrosequencing approach |
title_fullStr |
Diversity of toxin and non-toxin containing cyanobacterial mats of meltwater ponds on the Antarctic Peninsula: a pyrosequencing approach |
title_full_unstemmed |
Diversity of toxin and non-toxin containing cyanobacterial mats of meltwater ponds on the Antarctic Peninsula: a pyrosequencing approach |
title_sort |
diversity of toxin and non-toxin containing cyanobacterial mats of meltwater ponds on the antarctic peninsula: a pyrosequencing approach |
publisher |
Cambridge University Press |
publishDate |
2014 |
url |
https://nrl.northumbria.ac.uk/id/eprint/16958/ https://doi.org/10.1017/S0954102014000145 https://nrl.northumbria.ac.uk/id/eprint/16958/1/-ANS-S0954102014000145a.pdf |
long_lat |
ENVELOPE(-68.914,-68.914,-67.762,-67.762) |
geographic |
Adelaide Island Antarctic Antarctic Peninsula The Antarctic |
geographic_facet |
Adelaide Island Antarctic Antarctic Peninsula The Antarctic |
genre |
Adelaide Island Antarc* Antarctic Antarctic Peninsula Antarctic Science Antarctica |
genre_facet |
Adelaide Island Antarc* Antarctic Antarctic Peninsula Antarctic Science Antarctica |
op_relation |
https://nrl.northumbria.ac.uk/id/eprint/16958/1/-ANS-S0954102014000145a.pdf Kleinteich, Julia, Hildebrand, Falk, Wood, Susie, Cirés, Samuel, Agha, Ramsy, Quesada, Antonio, Pearce, David, Convey, Peter, Küpper, Frithjof and Dietrich, Daniel (2014) Diversity of toxin and non-toxin containing cyanobacterial mats of meltwater ponds on the Antarctic Peninsula: a pyrosequencing approach. Antarctic Science, 26 (5). pp. 521-532. ISSN 0954-1020 |
op_doi |
https://doi.org/10.1017/S0954102014000145 |
container_title |
Antarctic Science |
container_volume |
26 |
container_issue |
5 |
container_start_page |
521 |
op_container_end_page |
532 |
_version_ |
1766348726119432192 |