Detection of a novel ecotype of Pfiesteria piscicida (Dinophyceae) in an Antarctic saline lake by real-time PCR
The heterotrophic dinoflagellate Pfiesteria piscicida was detected in Ace Lake in the Vestfold Hills, eastern Antarctica by using real-time PCR based on 18S rDNA sequences. Antarctic water samples collected in 2004 were tested by species-specific real-time PCR assays for the identification of P. pis...
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ftunivnorthcag:oai:libres.uncg.edu/7577 2024-02-11T09:58:36+01:00 Detection of a novel ecotype of Pfiesteria piscicida (Dinophyceae) in an Antarctic saline lake by real-time PCR NC DOCKS at The University of North Carolina at Greensboro Rublee, Parke A. 2007 http://libres.uncg.edu/ir/uncg/f/P_Rublee_Detection_2007.pdf English eng http://libres.uncg.edu/ir/uncg/f/P_Rublee_Detection_2007.pdf 2007 ftunivnorthcag 2024-01-27T23:44:46Z The heterotrophic dinoflagellate Pfiesteria piscicida was detected in Ace Lake in the Vestfold Hills, eastern Antarctica by using real-time PCR based on 18S rDNA sequences. Antarctic water samples collected in 2004 were tested by species-specific real-time PCR assays for the identification of P. piscicida and P. shumwayae. Positive results were shown with P. piscicida-specific real-time PCR, and PCR products were examined by sequence analysis for confirmation. A phylogenetic tree made from partial 18S rDNA sequences showed that the Antarctic clone clustered with P. piscicida. This result suggests that P. piscicida is present in the extreme conditions of an Antarctic saline lake which has not contained fish for thousands of years. Other/Unknown Material Antarc* Antarctic Antarctica University of North Carolina: NC DOCKS (Digital Online Collection of Knowledge and Scholarship) Antarctic The Antarctic Vestfold Hills Vestfold Ace Lake ENVELOPE(78.188,78.188,-68.472,-68.472) Saline Lake ENVELOPE(-111.528,-111.528,57.081,57.081) |
institution |
Open Polar |
collection |
University of North Carolina: NC DOCKS (Digital Online Collection of Knowledge and Scholarship) |
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ftunivnorthcag |
language |
English |
description |
The heterotrophic dinoflagellate Pfiesteria piscicida was detected in Ace Lake in the Vestfold Hills, eastern Antarctica by using real-time PCR based on 18S rDNA sequences. Antarctic water samples collected in 2004 were tested by species-specific real-time PCR assays for the identification of P. piscicida and P. shumwayae. Positive results were shown with P. piscicida-specific real-time PCR, and PCR products were examined by sequence analysis for confirmation. A phylogenetic tree made from partial 18S rDNA sequences showed that the Antarctic clone clustered with P. piscicida. This result suggests that P. piscicida is present in the extreme conditions of an Antarctic saline lake which has not contained fish for thousands of years. |
author |
NC DOCKS at The University of North Carolina at Greensboro Rublee, Parke A. |
spellingShingle |
NC DOCKS at The University of North Carolina at Greensboro Rublee, Parke A. Detection of a novel ecotype of Pfiesteria piscicida (Dinophyceae) in an Antarctic saline lake by real-time PCR |
author_facet |
NC DOCKS at The University of North Carolina at Greensboro Rublee, Parke A. |
author_sort |
NC DOCKS at The University of North Carolina at Greensboro |
title |
Detection of a novel ecotype of Pfiesteria piscicida (Dinophyceae) in an Antarctic saline lake by real-time PCR |
title_short |
Detection of a novel ecotype of Pfiesteria piscicida (Dinophyceae) in an Antarctic saline lake by real-time PCR |
title_full |
Detection of a novel ecotype of Pfiesteria piscicida (Dinophyceae) in an Antarctic saline lake by real-time PCR |
title_fullStr |
Detection of a novel ecotype of Pfiesteria piscicida (Dinophyceae) in an Antarctic saline lake by real-time PCR |
title_full_unstemmed |
Detection of a novel ecotype of Pfiesteria piscicida (Dinophyceae) in an Antarctic saline lake by real-time PCR |
title_sort |
detection of a novel ecotype of pfiesteria piscicida (dinophyceae) in an antarctic saline lake by real-time pcr |
publishDate |
2007 |
url |
http://libres.uncg.edu/ir/uncg/f/P_Rublee_Detection_2007.pdf |
long_lat |
ENVELOPE(78.188,78.188,-68.472,-68.472) ENVELOPE(-111.528,-111.528,57.081,57.081) |
geographic |
Antarctic The Antarctic Vestfold Hills Vestfold Ace Lake Saline Lake |
geographic_facet |
Antarctic The Antarctic Vestfold Hills Vestfold Ace Lake Saline Lake |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_relation |
http://libres.uncg.edu/ir/uncg/f/P_Rublee_Detection_2007.pdf |
_version_ |
1790594306078146560 |