Anaerobic ammonium-oxidizing bacteria in marine environments: widespread occurrence but low diversity
Contains fulltext : 35120.pdf (Publisher’s version ) (Closed access) Laboratory and field studies have indicated that anaerobic ammonium oxidation (anammox) is an important process in the marine nitrogen cycle. In this study 11 additional anoxic marine sediment and water column samples were studied...
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ftunivnijmegen:oai:repository.ubn.ru.nl:2066/35120 2023-06-18T03:39:59+02:00 Anaerobic ammonium-oxidizing bacteria in marine environments: widespread occurrence but low diversity Schmid, M.C. Risgaard-Petersen, N. Vossenberg, J.L.C.M. van de Kuypers, M.M. Lavik, G. Petersen, J. Hulth, S. Thamdrup, B. Canfield, D. Dalsgaard, T. Rysgaard, S. Sejr, M.K. Strous, M. Camp, H.J.M. op den Jetten, M.S.M. 2007 http://hdl.handle.net/2066/35120 https://doi.org/10.1111/j.1462-2920.2007.01266.x unknown http://hdl.handle.net/2066/35120 doi:10.1111/j.1462-2920.2007.01266.x Environmental Microbiology, 9, 6, pp. 1476-1484 Ecological Microbiology Article / Letter to editor 2007 ftunivnijmegen https://doi.org/10.1111/j.1462-2920.2007.01266.x 2023-06-07T22:09:01Z Contains fulltext : 35120.pdf (Publisher’s version ) (Closed access) Laboratory and field studies have indicated that anaerobic ammonium oxidation (anammox) is an important process in the marine nitrogen cycle. In this study 11 additional anoxic marine sediment and water column samples were studied to substantiate this claim. In a combined approach using the molecular methods, polymerase chain reaction (PCR), qualitative and quantitative fluorescence in situ hybridization (FISH), as well as (15)N stable isotope activity measurements, it was shown that anammox bacteria were present and active in all samples investigated. The anammox activity measured in the sediment samples ranged from 0.08 fmol cell(-1) day(-1) N(2) in the Golfo Dulce (Pacific Ocean, Costa Rica) sediment to 0.98 fmol cell(-1) day(-1) N(2) in the Gullmarsfjorden (North Sea, Sweden) sediment. The percentage of anammox cell of the total population (stained with DAPI) as assessed by quantitative FISH was highest in the Barents Sea (9% +/- 4%) and in most of the samples well over 2%. Fluorescence in situ hybridization and phylogenetic analysis of the PCR products derived from the marine samples indicated the exclusive presence of members of the Candidatus'Scalindua' genus. This study showed the ubiquitous presence of anammox bacteria in anoxic marine ecosystems, supporting previous observations on the importance of anammox for N cycling in marine environments. Article in Journal/Newspaper Barents Sea Radboud University: DSpace Barents Sea Pacific Environmental Microbiology 9 6 1476 1484 |
institution |
Open Polar |
collection |
Radboud University: DSpace |
op_collection_id |
ftunivnijmegen |
language |
unknown |
topic |
Ecological Microbiology |
spellingShingle |
Ecological Microbiology Schmid, M.C. Risgaard-Petersen, N. Vossenberg, J.L.C.M. van de Kuypers, M.M. Lavik, G. Petersen, J. Hulth, S. Thamdrup, B. Canfield, D. Dalsgaard, T. Rysgaard, S. Sejr, M.K. Strous, M. Camp, H.J.M. op den Jetten, M.S.M. Anaerobic ammonium-oxidizing bacteria in marine environments: widespread occurrence but low diversity |
topic_facet |
Ecological Microbiology |
description |
Contains fulltext : 35120.pdf (Publisher’s version ) (Closed access) Laboratory and field studies have indicated that anaerobic ammonium oxidation (anammox) is an important process in the marine nitrogen cycle. In this study 11 additional anoxic marine sediment and water column samples were studied to substantiate this claim. In a combined approach using the molecular methods, polymerase chain reaction (PCR), qualitative and quantitative fluorescence in situ hybridization (FISH), as well as (15)N stable isotope activity measurements, it was shown that anammox bacteria were present and active in all samples investigated. The anammox activity measured in the sediment samples ranged from 0.08 fmol cell(-1) day(-1) N(2) in the Golfo Dulce (Pacific Ocean, Costa Rica) sediment to 0.98 fmol cell(-1) day(-1) N(2) in the Gullmarsfjorden (North Sea, Sweden) sediment. The percentage of anammox cell of the total population (stained with DAPI) as assessed by quantitative FISH was highest in the Barents Sea (9% +/- 4%) and in most of the samples well over 2%. Fluorescence in situ hybridization and phylogenetic analysis of the PCR products derived from the marine samples indicated the exclusive presence of members of the Candidatus'Scalindua' genus. This study showed the ubiquitous presence of anammox bacteria in anoxic marine ecosystems, supporting previous observations on the importance of anammox for N cycling in marine environments. |
format |
Article in Journal/Newspaper |
author |
Schmid, M.C. Risgaard-Petersen, N. Vossenberg, J.L.C.M. van de Kuypers, M.M. Lavik, G. Petersen, J. Hulth, S. Thamdrup, B. Canfield, D. Dalsgaard, T. Rysgaard, S. Sejr, M.K. Strous, M. Camp, H.J.M. op den Jetten, M.S.M. |
author_facet |
Schmid, M.C. Risgaard-Petersen, N. Vossenberg, J.L.C.M. van de Kuypers, M.M. Lavik, G. Petersen, J. Hulth, S. Thamdrup, B. Canfield, D. Dalsgaard, T. Rysgaard, S. Sejr, M.K. Strous, M. Camp, H.J.M. op den Jetten, M.S.M. |
author_sort |
Schmid, M.C. |
title |
Anaerobic ammonium-oxidizing bacteria in marine environments: widespread occurrence but low diversity |
title_short |
Anaerobic ammonium-oxidizing bacteria in marine environments: widespread occurrence but low diversity |
title_full |
Anaerobic ammonium-oxidizing bacteria in marine environments: widespread occurrence but low diversity |
title_fullStr |
Anaerobic ammonium-oxidizing bacteria in marine environments: widespread occurrence but low diversity |
title_full_unstemmed |
Anaerobic ammonium-oxidizing bacteria in marine environments: widespread occurrence but low diversity |
title_sort |
anaerobic ammonium-oxidizing bacteria in marine environments: widespread occurrence but low diversity |
publishDate |
2007 |
url |
http://hdl.handle.net/2066/35120 https://doi.org/10.1111/j.1462-2920.2007.01266.x |
geographic |
Barents Sea Pacific |
geographic_facet |
Barents Sea Pacific |
genre |
Barents Sea |
genre_facet |
Barents Sea |
op_source |
Environmental Microbiology, 9, 6, pp. 1476-1484 |
op_relation |
http://hdl.handle.net/2066/35120 doi:10.1111/j.1462-2920.2007.01266.x |
op_doi |
https://doi.org/10.1111/j.1462-2920.2007.01266.x |
container_title |
Environmental Microbiology |
container_volume |
9 |
container_issue |
6 |
container_start_page |
1476 |
op_container_end_page |
1484 |
_version_ |
1769004777350365184 |