A trace-metal clean, pH-controlled incubator system for ocean acidification incubation studies
The emerging research field of ocean acidification studies has gained international attention during the past years and recently defined international standards in the Guide to best practices for ocean acidification research and data reporting. However, a combination of ocean acidification studies w...
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American Society of Limnology and Oceanography Inc
2013
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ftunivnewengland:oai:rune.une.edu.au:1959.11/15836 2023-08-27T04:11:15+02:00 A trace-metal clean, pH-controlled incubator system for ocean acidification incubation studies Hoffmann, Linn J Breitbarth, Eike McGraw, Christina School of Science and Technology Law, Cliff S Currie, Kim I Hunter, Keith A 2013 https://hdl.handle.net/1959.11/15836 en eng American Society of Limnology and Oceanography Inc 10.4319/lom.2013.11.53 https://hdl.handle.net/1959.11/15836 une:16073 Gold Instrumental Methods (excl Immunological and Bioassay Methods) Global Change Biology Journal Article 2013 ftunivnewengland 2023-08-10T19:53:08Z The emerging research field of ocean acidification studies has gained international attention during the past years and recently defined international standards in the Guide to best practices for ocean acidification research and data reporting. However, a combination of ocean acidification studies with trace metal research is very rare and possible trace metal side effects on marine phytoplankton in ocean acidification incubation studies are often not assessed. Here we describe a trace metal clean, pH-controlled incubator system for laboratory and seagoing ocean acidification research. Seawater pH adjustment is achieved via passing CO₂ gas through diffusive silicone tubing to minimize the risk of contamination and to avoid the negative mechanical effects of gas bubbles on phytoplankton. The system measures pH automatically with an accuracy of 0.004 and a precision of 0.001 and includes a feedback regulation to adjust pH during the incubation if required. Mn, Fe, Co, Ni, Cd, and Pb measurements show that our system and the pH adjustment method do not contaminate the samples with any of these metals. We tested this system in laboratory studies as well as during the PINTS voyage in the Tasman Sea. Article in Journal/Newspaper Ocean acidification Research UNE - University of New England at Armidale, NSW Australia |
institution |
Open Polar |
collection |
Research UNE - University of New England at Armidale, NSW Australia |
op_collection_id |
ftunivnewengland |
language |
English |
topic |
Instrumental Methods (excl Immunological and Bioassay Methods) Global Change Biology |
spellingShingle |
Instrumental Methods (excl Immunological and Bioassay Methods) Global Change Biology Hoffmann, Linn J Breitbarth, Eike McGraw, Christina School of Science and Technology Law, Cliff S Currie, Kim I Hunter, Keith A A trace-metal clean, pH-controlled incubator system for ocean acidification incubation studies |
topic_facet |
Instrumental Methods (excl Immunological and Bioassay Methods) Global Change Biology |
description |
The emerging research field of ocean acidification studies has gained international attention during the past years and recently defined international standards in the Guide to best practices for ocean acidification research and data reporting. However, a combination of ocean acidification studies with trace metal research is very rare and possible trace metal side effects on marine phytoplankton in ocean acidification incubation studies are often not assessed. Here we describe a trace metal clean, pH-controlled incubator system for laboratory and seagoing ocean acidification research. Seawater pH adjustment is achieved via passing CO₂ gas through diffusive silicone tubing to minimize the risk of contamination and to avoid the negative mechanical effects of gas bubbles on phytoplankton. The system measures pH automatically with an accuracy of 0.004 and a precision of 0.001 and includes a feedback regulation to adjust pH during the incubation if required. Mn, Fe, Co, Ni, Cd, and Pb measurements show that our system and the pH adjustment method do not contaminate the samples with any of these metals. We tested this system in laboratory studies as well as during the PINTS voyage in the Tasman Sea. |
format |
Article in Journal/Newspaper |
author |
Hoffmann, Linn J Breitbarth, Eike McGraw, Christina School of Science and Technology Law, Cliff S Currie, Kim I Hunter, Keith A |
author_facet |
Hoffmann, Linn J Breitbarth, Eike McGraw, Christina School of Science and Technology Law, Cliff S Currie, Kim I Hunter, Keith A |
author_sort |
Hoffmann, Linn J |
title |
A trace-metal clean, pH-controlled incubator system for ocean acidification incubation studies |
title_short |
A trace-metal clean, pH-controlled incubator system for ocean acidification incubation studies |
title_full |
A trace-metal clean, pH-controlled incubator system for ocean acidification incubation studies |
title_fullStr |
A trace-metal clean, pH-controlled incubator system for ocean acidification incubation studies |
title_full_unstemmed |
A trace-metal clean, pH-controlled incubator system for ocean acidification incubation studies |
title_sort |
trace-metal clean, ph-controlled incubator system for ocean acidification incubation studies |
publisher |
American Society of Limnology and Oceanography Inc |
publishDate |
2013 |
url |
https://hdl.handle.net/1959.11/15836 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
10.4319/lom.2013.11.53 https://hdl.handle.net/1959.11/15836 une:16073 |
op_rights |
Gold |
_version_ |
1775353889448525824 |