Development and validation of a radioimmunoassay for fish insulin-like growth factor I (IGF-I) and the effect of aquaculture related stressors on circulating IGF-I levels

• Main Authors: Dyer, Anthony R, Upton, Zee, Stone, David, Thomas, Philip, Business Economics and Public Policy, Soole, Kathleen Lydia, Higgs, Naomi, Quinn, Kirsty, Carragher, John F
• Format: Article in Journal/Newspaper
• Language: English
• Published: Academic Press 2004
• Subjects: Biochemistry and Cell Biology; Atlantic salmon; Salmo salar
• Online Access: https://hdl.handle.net/1959.11/15224

This paper describes the development and validation of a commercially available radioimmunoassay (RIA) for the detection of fish insulin-like growth factor-I (IGF-I). The assay was developed using recombinant barramundi IGF-I as antigen and recombinant tuna IGF-I as radiolabelled tracer and standard. Assay sensitivity was 0.15 ng/ml, inter-assay variation was 16% (n=9) and intra-assay variation was 3% (n=10). Cross reactivity of less than 0.01% was found with salmon insulin, salmon IGF-II and barramundi IGF-II, less than 0.5% with human IGF-I and less than 1% with human IGF-II. Parallel dose-response inhibition curves were shown for barramundi ('Lates calcarifer'), coho salmon ('Oncorhynchus kisutch'), Southern Bluefin tuna ('Thunnus maccoyii'), tilapia ('Oreochromis mossambicus'), and seabream ('Pagrus auratus') IGF-I. The assay was then used to measure stress related changes in different aquacultured fish species. Salt water acclimated Atlantic salmon smolts ('Salmo salar') bathed for 2 h in fresh water showed significantly lower IGF-I concentrations than control smolts two days after the bath (53.1 compared to 32.1 ng/ml), with levels of IGF-I also lower in smolts exhibiting stunted growth (stunts). Capture and confinement of wild tuna in sea-cages resulted in a significant decrease in IGF-I levels (28 ng/ml) when compared to tuna captured and sampled immediately (48 ng/ml), but had recovered to starting levels after 3 weeks (43 ng/ml). Handling and isolation in silver perch ('Bidyanus bidyanus') led to a gradual decline in IGF-I over a 12 h period (36-19 ng/ml) but showed signs of recovery by 24 h (24 ng/ml) and had recovered fully 72 h after treatment (40 ng/ml). A similar trial in black bream ('Acanthopagrus butcherii') showed comparable results with IGF-I levels gradually decreasing (40-26 ng/ml) over 24 h, results that were mirrored by cortisol concentrations which increased during this time (1-26 ng/ml). In the studies presented here changes in IGF-I levels were not observed for at least 3 h after ...