Environmental DNA metabarcoding describes biodiversity across marine gradients
In response to climate change, biodiversity patterns in the oceans are predicted to shift rapidly, thus increasing the need for efficient monitoring methods. Environmental DNA (eDNA) metabarcoding recently emerged as a potent and cost-effective candidate to answer this challenge. We targeted three m...
Main Authors: | , , , , , , , , , , , , |
---|---|
Other Authors: | |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Oxford University Press
2023
|
Subjects: | |
Online Access: | http://hdl.handle.net/1959.13/1488248 |
id |
ftunivnewcastnsw:uon:52381 |
---|---|
record_format |
openpolar |
spelling |
ftunivnewcastnsw:uon:52381 2023-11-12T04:08:09+01:00 Environmental DNA metabarcoding describes biodiversity across marine gradients Adams, Clare I. M. Jeunen, Gert-Jan Stat, Michael Bunce, Michael Knapp, Michael Cross, Hugh Taylor, Helen R. Bagnaro, Antoine Currie, Kim Hepburn, Chris Gemmell, Neil J. Urban, Lara Baltar, Federico The University of Newcastle. College of Engineering, Science & Environment, School of Environmental and Life Sciences 2023 http://hdl.handle.net/1959.13/1488248 eng eng Oxford University Press ICES Journal of Marine Science Vol. 80, Issue 4, p. 953-971 10.1093/icesjms/fsad017 http://hdl.handle.net/1959.13/1488248 uon:52381 ISSN:1054-3139 x community biodiversity eDNA environmental DNA monitoring munida transect spatial heterogeneity journal article 2023 ftunivnewcastnsw 2023-10-16T22:27:01Z In response to climate change, biodiversity patterns in the oceans are predicted to shift rapidly, thus increasing the need for efficient monitoring methods. Environmental DNA (eDNA) metabarcoding recently emerged as a potent and cost-effective candidate to answer this challenge. We targeted three molecular markers to determine multicellular metazoan communities from two timepoints across a long-standing transect in the Southern Hemisphere, the Munida Observational Time Series. We detected four community types across the successive water masses—neritic, sub-tropical, frontal, and sub-Antarctic—crossed by the transect, together with important community differences between the two sampling points. From indicator species analysis, we found diversity patterns were mostly driven by planktonic organisms. Mesopelagic communities differed from surface-water communities in the sub-Antarctic water mass, with at-depth communities dominated by single-cellular organisms. We evaluate the ability of eDNA to detect species-compositional changes across surface and depth gradients and lay the foundations for using this technique in multi-trophic environmental monitoring efforts across long time series. We observed community differences across time and space. More intensive sampling will be critical to fully capture diversity across marine gradients, but this multi-trophic method represents an invaluable opportunity to understand shifts in marine biota. Article in Journal/Newspaper Antarc* Antarctic NOVA: The University of Newcastle Research Online (Australia) Antarctic |
institution |
Open Polar |
collection |
NOVA: The University of Newcastle Research Online (Australia) |
op_collection_id |
ftunivnewcastnsw |
language |
English |
topic |
community biodiversity eDNA environmental DNA monitoring munida transect spatial heterogeneity |
spellingShingle |
community biodiversity eDNA environmental DNA monitoring munida transect spatial heterogeneity Adams, Clare I. M. Jeunen, Gert-Jan Stat, Michael Bunce, Michael Knapp, Michael Cross, Hugh Taylor, Helen R. Bagnaro, Antoine Currie, Kim Hepburn, Chris Gemmell, Neil J. Urban, Lara Baltar, Federico Environmental DNA metabarcoding describes biodiversity across marine gradients |
topic_facet |
community biodiversity eDNA environmental DNA monitoring munida transect spatial heterogeneity |
description |
In response to climate change, biodiversity patterns in the oceans are predicted to shift rapidly, thus increasing the need for efficient monitoring methods. Environmental DNA (eDNA) metabarcoding recently emerged as a potent and cost-effective candidate to answer this challenge. We targeted three molecular markers to determine multicellular metazoan communities from two timepoints across a long-standing transect in the Southern Hemisphere, the Munida Observational Time Series. We detected four community types across the successive water masses—neritic, sub-tropical, frontal, and sub-Antarctic—crossed by the transect, together with important community differences between the two sampling points. From indicator species analysis, we found diversity patterns were mostly driven by planktonic organisms. Mesopelagic communities differed from surface-water communities in the sub-Antarctic water mass, with at-depth communities dominated by single-cellular organisms. We evaluate the ability of eDNA to detect species-compositional changes across surface and depth gradients and lay the foundations for using this technique in multi-trophic environmental monitoring efforts across long time series. We observed community differences across time and space. More intensive sampling will be critical to fully capture diversity across marine gradients, but this multi-trophic method represents an invaluable opportunity to understand shifts in marine biota. |
author2 |
The University of Newcastle. College of Engineering, Science & Environment, School of Environmental and Life Sciences |
format |
Article in Journal/Newspaper |
author |
Adams, Clare I. M. Jeunen, Gert-Jan Stat, Michael Bunce, Michael Knapp, Michael Cross, Hugh Taylor, Helen R. Bagnaro, Antoine Currie, Kim Hepburn, Chris Gemmell, Neil J. Urban, Lara Baltar, Federico |
author_facet |
Adams, Clare I. M. Jeunen, Gert-Jan Stat, Michael Bunce, Michael Knapp, Michael Cross, Hugh Taylor, Helen R. Bagnaro, Antoine Currie, Kim Hepburn, Chris Gemmell, Neil J. Urban, Lara Baltar, Federico |
author_sort |
Adams, Clare I. M. |
title |
Environmental DNA metabarcoding describes biodiversity across marine gradients |
title_short |
Environmental DNA metabarcoding describes biodiversity across marine gradients |
title_full |
Environmental DNA metabarcoding describes biodiversity across marine gradients |
title_fullStr |
Environmental DNA metabarcoding describes biodiversity across marine gradients |
title_full_unstemmed |
Environmental DNA metabarcoding describes biodiversity across marine gradients |
title_sort |
environmental dna metabarcoding describes biodiversity across marine gradients |
publisher |
Oxford University Press |
publishDate |
2023 |
url |
http://hdl.handle.net/1959.13/1488248 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
ICES Journal of Marine Science Vol. 80, Issue 4, p. 953-971 10.1093/icesjms/fsad017 http://hdl.handle.net/1959.13/1488248 uon:52381 ISSN:1054-3139 |
op_rights |
x |
_version_ |
1782328531580092416 |