Heat-shock protein adaptation in abyssal and hadal amphipods
© 2018 Elsevier Ltd Heat-shock proteins (HSPs) are a prominent family of cellular chaperones that are involved in the folding, assembly and degradation of cellular proteins, cell-cycling and signal transduction. HSPs are high conserved across taxa and form a key component of the stress response with...
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ftunivnewcastle:oai:eprint.ncl.ac.uk:248508 2023-05-15T15:06:13+02:00 Heat-shock protein adaptation in abyssal and hadal amphipods Ritchie H Jamieson AJ Piertney SB 2018 application/pdf https://eprint.ncl.ac.uk/fulltext.aspx?url=248508/85D33480-03C8-456E-BB85-13EB7C75A1C0.pdf&pub_id=248508 unknown Elsevier Ltd Deep Sea Research Part II: Topical Studies in Oceanography, 2018 Article 2018 ftunivnewcastle 2020-06-11T23:42:15Z © 2018 Elsevier Ltd Heat-shock proteins (HSPs) are a prominent family of cellular chaperones that are involved in the folding, assembly and degradation of cellular proteins, cell-cycling and signal transduction. HSPs are high conserved across taxa and form a key component of the stress response with signatures of molecular adaptation in some species exposed to extreme environmental stressors such as dehydration, heavy metal pollutants and arctic temperatures. Here we characterise two key heat-shock protein genes (hsp70 and hsp90) in deep-sea Lysianassoidea amphipods, with a focus on copy number variation and signatures of selection on the DNA sequences. Four phylogenetically distinct isoforms were resolved for both hsp70 and hsp90, with one isoform in each gene being exclusive to the hadal genus Hirondellea. Signatures of purifying selection were shown across hsp70 and hsp90 from dN:dS ratios. The GC content of each gene was lower, and the number of codons used was higher, than in shallow water amphipods suggesting a relaxation in codon usage bias. Such observations suggest that increased hydrostatic pressure is an important environmental stress that shapes the adaptation of heat-shock protein genes in deep-sea amphipods. Article in Journal/Newspaper Arctic Newcastle University Library ePrints Service Arctic |
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Newcastle University Library ePrints Service |
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© 2018 Elsevier Ltd Heat-shock proteins (HSPs) are a prominent family of cellular chaperones that are involved in the folding, assembly and degradation of cellular proteins, cell-cycling and signal transduction. HSPs are high conserved across taxa and form a key component of the stress response with signatures of molecular adaptation in some species exposed to extreme environmental stressors such as dehydration, heavy metal pollutants and arctic temperatures. Here we characterise two key heat-shock protein genes (hsp70 and hsp90) in deep-sea Lysianassoidea amphipods, with a focus on copy number variation and signatures of selection on the DNA sequences. Four phylogenetically distinct isoforms were resolved for both hsp70 and hsp90, with one isoform in each gene being exclusive to the hadal genus Hirondellea. Signatures of purifying selection were shown across hsp70 and hsp90 from dN:dS ratios. The GC content of each gene was lower, and the number of codons used was higher, than in shallow water amphipods suggesting a relaxation in codon usage bias. Such observations suggest that increased hydrostatic pressure is an important environmental stress that shapes the adaptation of heat-shock protein genes in deep-sea amphipods. |
format |
Article in Journal/Newspaper |
author |
Ritchie H Jamieson AJ Piertney SB |
spellingShingle |
Ritchie H Jamieson AJ Piertney SB Heat-shock protein adaptation in abyssal and hadal amphipods |
author_facet |
Ritchie H Jamieson AJ Piertney SB |
author_sort |
Ritchie H |
title |
Heat-shock protein adaptation in abyssal and hadal amphipods |
title_short |
Heat-shock protein adaptation in abyssal and hadal amphipods |
title_full |
Heat-shock protein adaptation in abyssal and hadal amphipods |
title_fullStr |
Heat-shock protein adaptation in abyssal and hadal amphipods |
title_full_unstemmed |
Heat-shock protein adaptation in abyssal and hadal amphipods |
title_sort |
heat-shock protein adaptation in abyssal and hadal amphipods |
publisher |
Elsevier Ltd |
publishDate |
2018 |
url |
https://eprint.ncl.ac.uk/fulltext.aspx?url=248508/85D33480-03C8-456E-BB85-13EB7C75A1C0.pdf&pub_id=248508 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Deep Sea Research Part II: Topical Studies in Oceanography, 2018 |
_version_ |
1766337868039454720 |