Polar compounds preclude mathematical lipid correction of carbon stable isotopes in deep-water sharks
© 2017 Elsevier B.V. Lipids affect stable isotope values generated for marine fishes, however these effects remain poorly described for many extant shark taxa, especially deep-sea species. Here, we report the effects of lipid extraction (LE) on δ13C, δ15N, and C:N values of seven deep-sea sharks, ge...
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ftunivnewcastle:oai:eprint.ncl.ac.uk:238898 2023-05-15T16:27:10+02:00 Polar compounds preclude mathematical lipid correction of carbon stable isotopes in deep-water sharks Shipley ON Olin JA Polunin NVC Sweeting CJ Newman SP Brooks EJ Barker S Witt MJ Talwar B Hussey NE September 2017 application/pdf https://eprint.ncl.ac.uk/fulltext.aspx?url=238898/4101CDDB-8E3E-4039-B87F-D1E418BF69F7.pdf&pub_id=238898 unknown Elsevier BV Journal of Experimental Marine Biology and Ecology, September 2017 Article 2017 ftunivnewcastle 2020-06-11T23:37:14Z © 2017 Elsevier B.V. Lipids affect stable isotope values generated for marine fishes, however these effects remain poorly described for many extant shark taxa, especially deep-sea species. Here, we report the effects of lipid extraction (LE) on δ13C, δ15N, and C:N values of seven deep-sea sharks, generate novel mathematical normalizations for δ13C based on the relationship between bulk and lipid extracted values (δ13CBulk and δ13CLE) and examine whether common normalized correction models provide a robust method for addressing lipid-biasing effects in two species, the Cuban dogfish (Squalus cubensis; n = 20), and Greenland shark (Somniosus microcephalus; n = 24). LE generally resulted in enrichment of 13C and 15N, but produced variable effects on C:N across all species. Novel mathematical normalizations for δ13C were derived from the pooled shark community, and a single species-specific correction was generated for the Cuban dogfish, but could not be determined for the Greenland shark. Four common lipid correction models used for teleosts, failed to accurately predict δ13C values statistically similar to δ13CLE, in both Cuban dogfish and Greenland sharks, likely due to the confounding effects of lipids and urea on C:N. These observations suggest that chemical lipid extraction should be a mandatory procedure prior to interpreting stable isotope data for deep-sea sharks, at least for those species where lipid effects are large. Article in Journal/Newspaper Greenland Somniosus microcephalus Newcastle University Library ePrints Service Greenland |
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Newcastle University Library ePrints Service |
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© 2017 Elsevier B.V. Lipids affect stable isotope values generated for marine fishes, however these effects remain poorly described for many extant shark taxa, especially deep-sea species. Here, we report the effects of lipid extraction (LE) on δ13C, δ15N, and C:N values of seven deep-sea sharks, generate novel mathematical normalizations for δ13C based on the relationship between bulk and lipid extracted values (δ13CBulk and δ13CLE) and examine whether common normalized correction models provide a robust method for addressing lipid-biasing effects in two species, the Cuban dogfish (Squalus cubensis; n = 20), and Greenland shark (Somniosus microcephalus; n = 24). LE generally resulted in enrichment of 13C and 15N, but produced variable effects on C:N across all species. Novel mathematical normalizations for δ13C were derived from the pooled shark community, and a single species-specific correction was generated for the Cuban dogfish, but could not be determined for the Greenland shark. Four common lipid correction models used for teleosts, failed to accurately predict δ13C values statistically similar to δ13CLE, in both Cuban dogfish and Greenland sharks, likely due to the confounding effects of lipids and urea on C:N. These observations suggest that chemical lipid extraction should be a mandatory procedure prior to interpreting stable isotope data for deep-sea sharks, at least for those species where lipid effects are large. |
format |
Article in Journal/Newspaper |
author |
Shipley ON Olin JA Polunin NVC Sweeting CJ Newman SP Brooks EJ Barker S Witt MJ Talwar B Hussey NE |
spellingShingle |
Shipley ON Olin JA Polunin NVC Sweeting CJ Newman SP Brooks EJ Barker S Witt MJ Talwar B Hussey NE Polar compounds preclude mathematical lipid correction of carbon stable isotopes in deep-water sharks |
author_facet |
Shipley ON Olin JA Polunin NVC Sweeting CJ Newman SP Brooks EJ Barker S Witt MJ Talwar B Hussey NE |
author_sort |
Shipley ON |
title |
Polar compounds preclude mathematical lipid correction of carbon stable isotopes in deep-water sharks |
title_short |
Polar compounds preclude mathematical lipid correction of carbon stable isotopes in deep-water sharks |
title_full |
Polar compounds preclude mathematical lipid correction of carbon stable isotopes in deep-water sharks |
title_fullStr |
Polar compounds preclude mathematical lipid correction of carbon stable isotopes in deep-water sharks |
title_full_unstemmed |
Polar compounds preclude mathematical lipid correction of carbon stable isotopes in deep-water sharks |
title_sort |
polar compounds preclude mathematical lipid correction of carbon stable isotopes in deep-water sharks |
publisher |
Elsevier BV |
publishDate |
2017 |
url |
https://eprint.ncl.ac.uk/fulltext.aspx?url=238898/4101CDDB-8E3E-4039-B87F-D1E418BF69F7.pdf&pub_id=238898 |
geographic |
Greenland |
geographic_facet |
Greenland |
genre |
Greenland Somniosus microcephalus |
genre_facet |
Greenland Somniosus microcephalus |
op_source |
Journal of Experimental Marine Biology and Ecology, September 2017 |
_version_ |
1766016257334706176 |