Tissue-specific molecular immune response to lipopolysaccharide challenge in emaciated anadromous Arctic charr
Anadromous Arctic charr (Salvelinus alpinus) undergo voluntary winter fasting for months in the Arctic. We tested the hypothesis that extended fasting will compromise the ability of this species to evoke an immune response. Charr were either fed or fasted for 85 days and challenged with lipopolysacc...
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ftunivnebraskali:oai:digitalcommons.unl.edu:usgsstaffpub-1851 2023-11-12T04:10:22+01:00 Tissue-specific molecular immune response to lipopolysaccharide challenge in emaciated anadromous Arctic charr Vijayan, Mathilakath Philip, Anju Jørgensen, Even Maule, Alec 2014-01-01T08:00:00Z application/pdf https://digitalcommons.unl.edu/usgsstaffpub/841 https://digitalcommons.unl.edu/context/usgsstaffpub/article/1851/viewcontent/Phillip_DCI_2014_Tissue_specific_molecular_immune_response.pdf unknown DigitalCommons@University of Nebraska - Lincoln https://digitalcommons.unl.edu/usgsstaffpub/841 https://digitalcommons.unl.edu/context/usgsstaffpub/article/1851/viewcontent/Phillip_DCI_2014_Tissue_specific_molecular_immune_response.pdf USGS Staff -- Published Research Fish Salvelinus alpinus Cytokines Serum amyloid protein A Suppressors of cytokine signalling SOCS text 2014 ftunivnebraskali 2023-10-30T11:10:45Z Anadromous Arctic charr (Salvelinus alpinus) undergo voluntary winter fasting for months in the Arctic. We tested the hypothesis that extended fasting will compromise the ability of this species to evoke an immune response. Charr were either fed or fasted for 85 days and challenged with lipopolysaccharide (LPS), and the molecular immune response in the liver and spleen assessed at 8 and 96 h post-injection. LPS increased IL-1β, IL-8, and serum amyloid protein A (SAA) mRNA levels in both groups, but the liver IL- 1β and IL-8, and spleen IL-8 responses were reduced in the fasted group. Fasting upregulated SOCS-1 and SOCS-2 mRNA abundance, while LPS stimulated SOCS-3 mRNA abundance and this response was higher in the fasted liver. Collectively, extended fasting and emaciation does not curtail the capacity of charr to evoke an immune response, whereas upregulation of SOCS may be a key adaptation to conserve energy by restricting the inflammatory response. Text Arctic charr Arctic Salvelinus alpinus University of Nebraska-Lincoln: DigitalCommons@UNL Arctic |
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Open Polar |
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University of Nebraska-Lincoln: DigitalCommons@UNL |
op_collection_id |
ftunivnebraskali |
language |
unknown |
topic |
Fish Salvelinus alpinus Cytokines Serum amyloid protein A Suppressors of cytokine signalling SOCS |
spellingShingle |
Fish Salvelinus alpinus Cytokines Serum amyloid protein A Suppressors of cytokine signalling SOCS Vijayan, Mathilakath Philip, Anju Jørgensen, Even Maule, Alec Tissue-specific molecular immune response to lipopolysaccharide challenge in emaciated anadromous Arctic charr |
topic_facet |
Fish Salvelinus alpinus Cytokines Serum amyloid protein A Suppressors of cytokine signalling SOCS |
description |
Anadromous Arctic charr (Salvelinus alpinus) undergo voluntary winter fasting for months in the Arctic. We tested the hypothesis that extended fasting will compromise the ability of this species to evoke an immune response. Charr were either fed or fasted for 85 days and challenged with lipopolysaccharide (LPS), and the molecular immune response in the liver and spleen assessed at 8 and 96 h post-injection. LPS increased IL-1β, IL-8, and serum amyloid protein A (SAA) mRNA levels in both groups, but the liver IL- 1β and IL-8, and spleen IL-8 responses were reduced in the fasted group. Fasting upregulated SOCS-1 and SOCS-2 mRNA abundance, while LPS stimulated SOCS-3 mRNA abundance and this response was higher in the fasted liver. Collectively, extended fasting and emaciation does not curtail the capacity of charr to evoke an immune response, whereas upregulation of SOCS may be a key adaptation to conserve energy by restricting the inflammatory response. |
format |
Text |
author |
Vijayan, Mathilakath Philip, Anju Jørgensen, Even Maule, Alec |
author_facet |
Vijayan, Mathilakath Philip, Anju Jørgensen, Even Maule, Alec |
author_sort |
Vijayan, Mathilakath |
title |
Tissue-specific molecular immune response to lipopolysaccharide challenge in emaciated anadromous Arctic charr |
title_short |
Tissue-specific molecular immune response to lipopolysaccharide challenge in emaciated anadromous Arctic charr |
title_full |
Tissue-specific molecular immune response to lipopolysaccharide challenge in emaciated anadromous Arctic charr |
title_fullStr |
Tissue-specific molecular immune response to lipopolysaccharide challenge in emaciated anadromous Arctic charr |
title_full_unstemmed |
Tissue-specific molecular immune response to lipopolysaccharide challenge in emaciated anadromous Arctic charr |
title_sort |
tissue-specific molecular immune response to lipopolysaccharide challenge in emaciated anadromous arctic charr |
publisher |
DigitalCommons@University of Nebraska - Lincoln |
publishDate |
2014 |
url |
https://digitalcommons.unl.edu/usgsstaffpub/841 https://digitalcommons.unl.edu/context/usgsstaffpub/article/1851/viewcontent/Phillip_DCI_2014_Tissue_specific_molecular_immune_response.pdf |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic charr Arctic Salvelinus alpinus |
genre_facet |
Arctic charr Arctic Salvelinus alpinus |
op_source |
USGS Staff -- Published Research |
op_relation |
https://digitalcommons.unl.edu/usgsstaffpub/841 https://digitalcommons.unl.edu/context/usgsstaffpub/article/1851/viewcontent/Phillip_DCI_2014_Tissue_specific_molecular_immune_response.pdf |
_version_ |
1782329878302949376 |