Differential Expression of Immune Response Genes in Steller Sea Lions ( Eumetopias jubatus ): An Indicator of Ecosystem Health?
Characterization of the polygenic and polymorphic features of the Steller sea lion major histocompatibility complex (MHC) provides an ideal window for evaluating immunologic vigor of the population and identifying emergence of new genotypes that reflect ecosystem pressures. MHC genotyping can be use...
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2006
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ftunivnebraskali:oai:digitalcommons.unl.edu:usdeptcommercepub-1180 2023-11-12T04:28:10+01:00 Differential Expression of Immune Response Genes in Steller Sea Lions ( Eumetopias jubatus ): An Indicator of Ecosystem Health? Bowen, Lizabeth Aldridge, Brian Beckmen, Kimberlee Gelatt, Tom Rea, Lorrie Burek, Kathy Pitcher, Ken Stott, Jeffrey L. 2006-01-01T08:00:00Z application/pdf https://digitalcommons.unl.edu/usdeptcommercepub/204 https://digitalcommons.unl.edu/context/usdeptcommercepub/article/1180/viewcontent/Gelatt_EH_2006_Differential_expression.pdf unknown DigitalCommons@University of Nebraska - Lincoln https://digitalcommons.unl.edu/usdeptcommercepub/204 https://digitalcommons.unl.edu/context/usdeptcommercepub/article/1180/viewcontent/Gelatt_EH_2006_Differential_expression.pdf Publications, Agencies and Staff of the U.S. Department of Commerce Environmental Health Environmental Indicators and Impact Assessment Environmental Sciences Marine Biology Other Immunology and Infectious Disease Other Oceanography and Atmospheric Sciences and Meteorology Zoology text 2006 ftunivnebraskali 2023-10-30T09:43:17Z Characterization of the polygenic and polymorphic features of the Steller sea lion major histocompatibility complex (MHC) provides an ideal window for evaluating immunologic vigor of the population and identifying emergence of new genotypes that reflect ecosystem pressures. MHC genotyping can be used to measure the potential immunologic vigor of a population. However, since ecosystem-induced changes to MHC genotype can be slow to emerge, measurement of differential expression of these genes can potentially provide real-time evidence of immunologic perturbations. MHC DRB genes were cloned and sequenced using peripheral blood mononuclear leukocytes derived from 10 Steller sea lions from southeast Alaska, Prince William Sound, and the Aleutian Islands. Nine unique DRB gene sequences were represented in each of ten animals. MHC DRB gene expression was measured in a subset of six sea lions. Although DRB in genomic DNA was identical in all individuals, relative levels of expressed DRB mRNA was highly variable. Selective suppression of MHC DRB genes could be indicative of geographically disparate environmental pressures, thereby serving as an immediate and sensitive indicator of population and ecosystem health. Text Alaska Aleutian Islands University of Nebraska-Lincoln: DigitalCommons@UNL |
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Open Polar |
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University of Nebraska-Lincoln: DigitalCommons@UNL |
op_collection_id |
ftunivnebraskali |
language |
unknown |
topic |
Environmental Health Environmental Indicators and Impact Assessment Environmental Sciences Marine Biology Other Immunology and Infectious Disease Other Oceanography and Atmospheric Sciences and Meteorology Zoology |
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Environmental Health Environmental Indicators and Impact Assessment Environmental Sciences Marine Biology Other Immunology and Infectious Disease Other Oceanography and Atmospheric Sciences and Meteorology Zoology Bowen, Lizabeth Aldridge, Brian Beckmen, Kimberlee Gelatt, Tom Rea, Lorrie Burek, Kathy Pitcher, Ken Stott, Jeffrey L. Differential Expression of Immune Response Genes in Steller Sea Lions ( Eumetopias jubatus ): An Indicator of Ecosystem Health? |
topic_facet |
Environmental Health Environmental Indicators and Impact Assessment Environmental Sciences Marine Biology Other Immunology and Infectious Disease Other Oceanography and Atmospheric Sciences and Meteorology Zoology |
description |
Characterization of the polygenic and polymorphic features of the Steller sea lion major histocompatibility complex (MHC) provides an ideal window for evaluating immunologic vigor of the population and identifying emergence of new genotypes that reflect ecosystem pressures. MHC genotyping can be used to measure the potential immunologic vigor of a population. However, since ecosystem-induced changes to MHC genotype can be slow to emerge, measurement of differential expression of these genes can potentially provide real-time evidence of immunologic perturbations. MHC DRB genes were cloned and sequenced using peripheral blood mononuclear leukocytes derived from 10 Steller sea lions from southeast Alaska, Prince William Sound, and the Aleutian Islands. Nine unique DRB gene sequences were represented in each of ten animals. MHC DRB gene expression was measured in a subset of six sea lions. Although DRB in genomic DNA was identical in all individuals, relative levels of expressed DRB mRNA was highly variable. Selective suppression of MHC DRB genes could be indicative of geographically disparate environmental pressures, thereby serving as an immediate and sensitive indicator of population and ecosystem health. |
format |
Text |
author |
Bowen, Lizabeth Aldridge, Brian Beckmen, Kimberlee Gelatt, Tom Rea, Lorrie Burek, Kathy Pitcher, Ken Stott, Jeffrey L. |
author_facet |
Bowen, Lizabeth Aldridge, Brian Beckmen, Kimberlee Gelatt, Tom Rea, Lorrie Burek, Kathy Pitcher, Ken Stott, Jeffrey L. |
author_sort |
Bowen, Lizabeth |
title |
Differential Expression of Immune Response Genes in Steller Sea Lions ( Eumetopias jubatus ): An Indicator of Ecosystem Health? |
title_short |
Differential Expression of Immune Response Genes in Steller Sea Lions ( Eumetopias jubatus ): An Indicator of Ecosystem Health? |
title_full |
Differential Expression of Immune Response Genes in Steller Sea Lions ( Eumetopias jubatus ): An Indicator of Ecosystem Health? |
title_fullStr |
Differential Expression of Immune Response Genes in Steller Sea Lions ( Eumetopias jubatus ): An Indicator of Ecosystem Health? |
title_full_unstemmed |
Differential Expression of Immune Response Genes in Steller Sea Lions ( Eumetopias jubatus ): An Indicator of Ecosystem Health? |
title_sort |
differential expression of immune response genes in steller sea lions ( eumetopias jubatus ): an indicator of ecosystem health? |
publisher |
DigitalCommons@University of Nebraska - Lincoln |
publishDate |
2006 |
url |
https://digitalcommons.unl.edu/usdeptcommercepub/204 https://digitalcommons.unl.edu/context/usdeptcommercepub/article/1180/viewcontent/Gelatt_EH_2006_Differential_expression.pdf |
genre |
Alaska Aleutian Islands |
genre_facet |
Alaska Aleutian Islands |
op_source |
Publications, Agencies and Staff of the U.S. Department of Commerce |
op_relation |
https://digitalcommons.unl.edu/usdeptcommercepub/204 https://digitalcommons.unl.edu/context/usdeptcommercepub/article/1180/viewcontent/Gelatt_EH_2006_Differential_expression.pdf |
_version_ |
1782341469795778560 |