Chloroplast DNA and Nuclear DNA Content Variations among Cultivars of Switchgrass, Panicum virgatum L.
Switchgrass, Panicum virgatum L., is a native, cross-pollinated, morphologically diverse species with an array of ploidy levels and ecotypes. Switchgrass is found throughout most of the USA and Canada, primarily east of the Rocky Mountains and south of Hudson Bay. The objective of this research was...
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ftunivnebraskali:oai:digitalcommons.unl.edu:usdaarsfacpub-2909 2023-11-12T04:18:18+01:00 Chloroplast DNA and Nuclear DNA Content Variations among Cultivars of Switchgrass, Panicum virgatum L. Hultquist, Sherry J. Vogel, Kenneth P. Lee, D. J. Arumuganathan, K. Kaeppler, S. 1996-01-01T08:00:00Z application/pdf https://digitalcommons.unl.edu/usdaarsfacpub/1896 https://digitalcommons.unl.edu/context/usdaarsfacpub/article/2909/viewcontent/Hultquist_Switchgrass_cultivars_DNA_1996.pdf unknown DigitalCommons@University of Nebraska - Lincoln https://digitalcommons.unl.edu/usdaarsfacpub/1896 https://digitalcommons.unl.edu/context/usdaarsfacpub/article/2909/viewcontent/Hultquist_Switchgrass_cultivars_DNA_1996.pdf Publications from USDA-ARS / UNL Faculty text 1996 ftunivnebraskali 2023-10-30T09:55:18Z Switchgrass, Panicum virgatum L., is a native, cross-pollinated, morphologically diverse species with an array of ploidy levels and ecotypes. Switchgrass is found throughout most of the USA and Canada, primarily east of the Rocky Mountains and south of Hudson Bay. The objective of this research was to determine if chloroplast DNA (cpDNA) restriction fragment length polymorphisms (RFLPs) occur among switchgrass cultivars and experimental strains that differ in ploidy level or ecotype classification. Eighteen switchgrass cultivars or experimental strains representative of reported ecotypes, ploidy levels, and the geographical range of switchgrass were surveyed for cpDNA polymorphisms by means of four restriction endonucleases and 20 sorghum cpDNA probes. One polymorphism was detected which was associated with the lowland-upland ecotype classification. The lowland cultivars contained a restriction site change that was not present in the upland cultivars. The two cytotypes discovered have been designated as the U (upland) or L (lowland) cytotype. The lowland cultivars had 3 pg DNA/nuclei as measured by flow cytometry while the upland types had either 3 or 6 pg DNA/nuclei. There were no cpDNA polymorphisms among the upland cultivars regardless of ploidy level as measured by DNA content. These results demonstrate that cpDNA differs among switchgrasses and that this variation is associated with ecotype variation but not with nuclear DNA content. Text Hudson Bay University of Nebraska-Lincoln: DigitalCommons@UNL Hudson Bay Canada Hudson |
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University of Nebraska-Lincoln: DigitalCommons@UNL |
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description |
Switchgrass, Panicum virgatum L., is a native, cross-pollinated, morphologically diverse species with an array of ploidy levels and ecotypes. Switchgrass is found throughout most of the USA and Canada, primarily east of the Rocky Mountains and south of Hudson Bay. The objective of this research was to determine if chloroplast DNA (cpDNA) restriction fragment length polymorphisms (RFLPs) occur among switchgrass cultivars and experimental strains that differ in ploidy level or ecotype classification. Eighteen switchgrass cultivars or experimental strains representative of reported ecotypes, ploidy levels, and the geographical range of switchgrass were surveyed for cpDNA polymorphisms by means of four restriction endonucleases and 20 sorghum cpDNA probes. One polymorphism was detected which was associated with the lowland-upland ecotype classification. The lowland cultivars contained a restriction site change that was not present in the upland cultivars. The two cytotypes discovered have been designated as the U (upland) or L (lowland) cytotype. The lowland cultivars had 3 pg DNA/nuclei as measured by flow cytometry while the upland types had either 3 or 6 pg DNA/nuclei. There were no cpDNA polymorphisms among the upland cultivars regardless of ploidy level as measured by DNA content. These results demonstrate that cpDNA differs among switchgrasses and that this variation is associated with ecotype variation but not with nuclear DNA content. |
format |
Text |
author |
Hultquist, Sherry J. Vogel, Kenneth P. Lee, D. J. Arumuganathan, K. Kaeppler, S. |
spellingShingle |
Hultquist, Sherry J. Vogel, Kenneth P. Lee, D. J. Arumuganathan, K. Kaeppler, S. Chloroplast DNA and Nuclear DNA Content Variations among Cultivars of Switchgrass, Panicum virgatum L. |
author_facet |
Hultquist, Sherry J. Vogel, Kenneth P. Lee, D. J. Arumuganathan, K. Kaeppler, S. |
author_sort |
Hultquist, Sherry J. |
title |
Chloroplast DNA and Nuclear DNA Content Variations among Cultivars of Switchgrass, Panicum virgatum L. |
title_short |
Chloroplast DNA and Nuclear DNA Content Variations among Cultivars of Switchgrass, Panicum virgatum L. |
title_full |
Chloroplast DNA and Nuclear DNA Content Variations among Cultivars of Switchgrass, Panicum virgatum L. |
title_fullStr |
Chloroplast DNA and Nuclear DNA Content Variations among Cultivars of Switchgrass, Panicum virgatum L. |
title_full_unstemmed |
Chloroplast DNA and Nuclear DNA Content Variations among Cultivars of Switchgrass, Panicum virgatum L. |
title_sort |
chloroplast dna and nuclear dna content variations among cultivars of switchgrass, panicum virgatum l. |
publisher |
DigitalCommons@University of Nebraska - Lincoln |
publishDate |
1996 |
url |
https://digitalcommons.unl.edu/usdaarsfacpub/1896 https://digitalcommons.unl.edu/context/usdaarsfacpub/article/2909/viewcontent/Hultquist_Switchgrass_cultivars_DNA_1996.pdf |
geographic |
Hudson Bay Canada Hudson |
geographic_facet |
Hudson Bay Canada Hudson |
genre |
Hudson Bay |
genre_facet |
Hudson Bay |
op_source |
Publications from USDA-ARS / UNL Faculty |
op_relation |
https://digitalcommons.unl.edu/usdaarsfacpub/1896 https://digitalcommons.unl.edu/context/usdaarsfacpub/article/2909/viewcontent/Hultquist_Switchgrass_cultivars_DNA_1996.pdf |
_version_ |
1782334947885842432 |