Complete Genome Sequence of an Avian Bornavirus Isolated from a Healthy Canadian Goose ( Branta canadensis )

Avian bornavirus (ABV) is the causative agent of proventricular dilatation disease, a fatal neurologic and gastrointestinal disease in psittacine and other birds (1, 2). The genomic sequences of different ABV isolates are much more variable than those of Borna disease virus (BDV) isolates (3). Recen...

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Main Authors: Guo, Jianhua, Baroch, John, Randall, Adam, Tizard, Ian
Format: Text
Language:unknown
Published: DigitalCommons@University of Nebraska - Lincoln 2013
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Online Access:https://digitalcommons.unl.edu/icwdm_usdanwrc/1480
https://digitalcommons.unl.edu/context/icwdm_usdanwrc/article/2499/viewcontent/Guo_GA_2013_Complete_genome.pdf
id ftunivnebraskali:oai:digitalcommons.unl.edu:icwdm_usdanwrc-2499
record_format openpolar
spelling ftunivnebraskali:oai:digitalcommons.unl.edu:icwdm_usdanwrc-2499 2023-11-12T04:15:26+01:00 Complete Genome Sequence of an Avian Bornavirus Isolated from a Healthy Canadian Goose ( Branta canadensis ) Guo, Jianhua Baroch, John Randall, Adam Tizard, Ian 2013-01-01T08:00:00Z application/pdf https://digitalcommons.unl.edu/icwdm_usdanwrc/1480 https://digitalcommons.unl.edu/context/icwdm_usdanwrc/article/2499/viewcontent/Guo_GA_2013_Complete_genome.pdf unknown DigitalCommons@University of Nebraska - Lincoln https://digitalcommons.unl.edu/icwdm_usdanwrc/1480 https://digitalcommons.unl.edu/context/icwdm_usdanwrc/article/2499/viewcontent/Guo_GA_2013_Complete_genome.pdf USDA Wildlife Services - Staff Publications Life Sciences text 2013 ftunivnebraskali 2023-10-30T11:16:53Z Avian bornavirus (ABV) is the causative agent of proventricular dilatation disease, a fatal neurologic and gastrointestinal disease in psittacine and other birds (1, 2). The genomic sequences of different ABV isolates are much more variable than those of Borna disease virus (BDV) isolates (3). Recent surveys have demonstrated that a unique genotype of ABV also infects apparently healthy geese and swans across North America (4–6). Under some circumstances, it has been reported to cause lethal neurological disease in Canadian geese and trumpeter swans (7, 8). The viruses that infect geese and swans are apparently from a single genotype. We report here on the complete genomic sequence of ABV isolated from the brain of a healthy Canadian goose (Branta canadensis), collected in Union County, New Jersey, on 14 February 2011. In this study, an ABV-positive goose brain sample was initially identified using a reverse transcriptase PCR (RT-PCR) employing primers targeting the M gene, as described previously (5, 6). A brain sample from this bird was then used to inoculate primary duck embryo fibroblasts (DEF). Following 3 serial passages, ABV growth was detected using RT-PCR. Two PCR products, approximately 3 and 6 kb in size, were cloned into the pCRTM4-TOPO vector and sequenced using a primer-walking approach by the Gene Technology Lab of Texas A&M University. The sequences were assembled with Sequencher 4.1, and phylogenetic analysis was performed using MEGA5.2. Text Branta canadensis University of Nebraska-Lincoln: DigitalCommons@UNL
institution Open Polar
collection University of Nebraska-Lincoln: DigitalCommons@UNL
op_collection_id ftunivnebraskali
language unknown
topic Life Sciences
spellingShingle Life Sciences
Guo, Jianhua
Baroch, John
Randall, Adam
Tizard, Ian
Complete Genome Sequence of an Avian Bornavirus Isolated from a Healthy Canadian Goose ( Branta canadensis )
topic_facet Life Sciences
description Avian bornavirus (ABV) is the causative agent of proventricular dilatation disease, a fatal neurologic and gastrointestinal disease in psittacine and other birds (1, 2). The genomic sequences of different ABV isolates are much more variable than those of Borna disease virus (BDV) isolates (3). Recent surveys have demonstrated that a unique genotype of ABV also infects apparently healthy geese and swans across North America (4–6). Under some circumstances, it has been reported to cause lethal neurological disease in Canadian geese and trumpeter swans (7, 8). The viruses that infect geese and swans are apparently from a single genotype. We report here on the complete genomic sequence of ABV isolated from the brain of a healthy Canadian goose (Branta canadensis), collected in Union County, New Jersey, on 14 February 2011. In this study, an ABV-positive goose brain sample was initially identified using a reverse transcriptase PCR (RT-PCR) employing primers targeting the M gene, as described previously (5, 6). A brain sample from this bird was then used to inoculate primary duck embryo fibroblasts (DEF). Following 3 serial passages, ABV growth was detected using RT-PCR. Two PCR products, approximately 3 and 6 kb in size, were cloned into the pCRTM4-TOPO vector and sequenced using a primer-walking approach by the Gene Technology Lab of Texas A&M University. The sequences were assembled with Sequencher 4.1, and phylogenetic analysis was performed using MEGA5.2.
format Text
author Guo, Jianhua
Baroch, John
Randall, Adam
Tizard, Ian
author_facet Guo, Jianhua
Baroch, John
Randall, Adam
Tizard, Ian
author_sort Guo, Jianhua
title Complete Genome Sequence of an Avian Bornavirus Isolated from a Healthy Canadian Goose ( Branta canadensis )
title_short Complete Genome Sequence of an Avian Bornavirus Isolated from a Healthy Canadian Goose ( Branta canadensis )
title_full Complete Genome Sequence of an Avian Bornavirus Isolated from a Healthy Canadian Goose ( Branta canadensis )
title_fullStr Complete Genome Sequence of an Avian Bornavirus Isolated from a Healthy Canadian Goose ( Branta canadensis )
title_full_unstemmed Complete Genome Sequence of an Avian Bornavirus Isolated from a Healthy Canadian Goose ( Branta canadensis )
title_sort complete genome sequence of an avian bornavirus isolated from a healthy canadian goose ( branta canadensis )
publisher DigitalCommons@University of Nebraska - Lincoln
publishDate 2013
url https://digitalcommons.unl.edu/icwdm_usdanwrc/1480
https://digitalcommons.unl.edu/context/icwdm_usdanwrc/article/2499/viewcontent/Guo_GA_2013_Complete_genome.pdf
genre Branta canadensis
genre_facet Branta canadensis
op_source USDA Wildlife Services - Staff Publications
op_relation https://digitalcommons.unl.edu/icwdm_usdanwrc/1480
https://digitalcommons.unl.edu/context/icwdm_usdanwrc/article/2499/viewcontent/Guo_GA_2013_Complete_genome.pdf
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