Detection and Genetic Characterization of Canine Adenoviruses, Circoviruses, and Novel Cycloviruses From Wild Carnivores in Italy
: Wild carnivores are known to play a role in the epidemiology of several canine viruses, including canine adenoviruses types 1 (CAdV-1) and 2 (CAdV-2), canine circovirus (CanineCV) and canine distemper virus (CDV). In the present study, we report an epidemiological survey for these viruses in free...
Published in: | Frontiers in Veterinary Science |
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Online Access: | https://hdl.handle.net/11588/943423 https://doi.org/10.3389/fvets.2022.851987 |
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ftunivnapoliiris:oai:www.iris.unina.it:11588/943423 2024-09-09T19:35:47+00:00 Detection and Genetic Characterization of Canine Adenoviruses, Circoviruses, and Novel Cycloviruses From Wild Carnivores in Italy Ndiana, Linda A Lanave, Gianvito Vasinioti, Violetta Desario, Costantina Martino, Camillo Colaianni, Maria Loredana Pellegrini, Francesco Camarda, Antonio Berjaoui, Shadia Sgroi, Giovanni Elia, Gabriella Pratelli, Annamaria Buono, Francesco Martella, Vito Buonavoglia, Canio Decaro, Nicola Ndiana, Linda A Lanave, Gianvito Vasinioti, Violetta Desario, Costantina Martino, Camillo Colaianni, Maria Loredana Pellegrini, Francesco Camarda, Antonio Berjaoui, Shadia Sgroi, Giovanni Elia, Gabriella Pratelli, Annamaria Buono, Francesco Martella, Vito Buonavoglia, Canio Decaro, Nicola 2022 https://hdl.handle.net/11588/943423 https://doi.org/10.3389/fvets.2022.851987 eng eng info:eu-repo/semantics/altIdentifier/wos/WOS:000787912300001 volume:9 firstpage:851987 journal:FRONTIERS IN VETERINARY SCIENCE https://hdl.handle.net/11588/943423 doi:10.3389/fvets.2022.851987 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85128700868 canine adenovirus type 1 canine circoviru cycloviruse molecular survey wild carnivores info:eu-repo/semantics/article 2022 ftunivnapoliiris https://doi.org/10.3389/fvets.2022.851987 2024-06-17T15:19:20Z : Wild carnivores are known to play a role in the epidemiology of several canine viruses, including canine adenoviruses types 1 (CAdV-1) and 2 (CAdV-2), canine circovirus (CanineCV) and canine distemper virus (CDV). In the present study, we report an epidemiological survey for these viruses in free ranging carnivores from Italy. A total of 262 wild carnivores, including red foxes (Vulpes vulpes), wolves (Canis lupus) and Eurasian badgers (Meles meles) were sampled. Viral nucleic acid was extracted and screened by real-time PCR assays (qPCR) for the presence of CAdVs and CanineCV DNA, as well as for CDV RNA. CAdV-1 DNA was detected only in red foxes (4/232, 1.7%) whilst the wolves (0/8, 0%) and Eurasian badgers (0/22, 0%) tested negative. CanineCV DNA was detected in 4 (18%) Eurasian badgers, 4 (50%) wolves and 0 (0%) red foxes. None of the animals tested positive for CDV or CAdV-2. By sequence and phylogenetic analyses, CAdV-1 and CanineCV sequences from wild carnivores were closely related to reference sequences from domestic dogs and wild carnivores. Surprisingly, two sequences from wolf intestines were identified as cycloviruses with one sequence (145.20-5432) displaying 68.6% nucleotide identity to a cyclovirus detected in a domestic cat, while the other (145.201329) was more closely related (79.4% nucleotide identity) to a cyclovirus sequence from bats. A continuous surveillance in wild carnivores should be carried out in order to monitor the circulation in wildlife of viruses pathogenic for domestic carnivores and endangered wild species. Article in Journal/Newspaper Canis lupus IRIS Università degli Studi di Napoli Federico II Frontiers in Veterinary Science 9 |
institution |
Open Polar |
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IRIS Università degli Studi di Napoli Federico II |
op_collection_id |
ftunivnapoliiris |
language |
English |
topic |
canine adenovirus type 1 canine circoviru cycloviruse molecular survey wild carnivores |
spellingShingle |
canine adenovirus type 1 canine circoviru cycloviruse molecular survey wild carnivores Ndiana, Linda A Lanave, Gianvito Vasinioti, Violetta Desario, Costantina Martino, Camillo Colaianni, Maria Loredana Pellegrini, Francesco Camarda, Antonio Berjaoui, Shadia Sgroi, Giovanni Elia, Gabriella Pratelli, Annamaria Buono, Francesco Martella, Vito Buonavoglia, Canio Decaro, Nicola Detection and Genetic Characterization of Canine Adenoviruses, Circoviruses, and Novel Cycloviruses From Wild Carnivores in Italy |
topic_facet |
canine adenovirus type 1 canine circoviru cycloviruse molecular survey wild carnivores |
description |
: Wild carnivores are known to play a role in the epidemiology of several canine viruses, including canine adenoviruses types 1 (CAdV-1) and 2 (CAdV-2), canine circovirus (CanineCV) and canine distemper virus (CDV). In the present study, we report an epidemiological survey for these viruses in free ranging carnivores from Italy. A total of 262 wild carnivores, including red foxes (Vulpes vulpes), wolves (Canis lupus) and Eurasian badgers (Meles meles) were sampled. Viral nucleic acid was extracted and screened by real-time PCR assays (qPCR) for the presence of CAdVs and CanineCV DNA, as well as for CDV RNA. CAdV-1 DNA was detected only in red foxes (4/232, 1.7%) whilst the wolves (0/8, 0%) and Eurasian badgers (0/22, 0%) tested negative. CanineCV DNA was detected in 4 (18%) Eurasian badgers, 4 (50%) wolves and 0 (0%) red foxes. None of the animals tested positive for CDV or CAdV-2. By sequence and phylogenetic analyses, CAdV-1 and CanineCV sequences from wild carnivores were closely related to reference sequences from domestic dogs and wild carnivores. Surprisingly, two sequences from wolf intestines were identified as cycloviruses with one sequence (145.20-5432) displaying 68.6% nucleotide identity to a cyclovirus detected in a domestic cat, while the other (145.201329) was more closely related (79.4% nucleotide identity) to a cyclovirus sequence from bats. A continuous surveillance in wild carnivores should be carried out in order to monitor the circulation in wildlife of viruses pathogenic for domestic carnivores and endangered wild species. |
author2 |
Ndiana, Linda A Lanave, Gianvito Vasinioti, Violetta Desario, Costantina Martino, Camillo Colaianni, Maria Loredana Pellegrini, Francesco Camarda, Antonio Berjaoui, Shadia Sgroi, Giovanni Elia, Gabriella Pratelli, Annamaria Buono, Francesco Martella, Vito Buonavoglia, Canio Decaro, Nicola |
format |
Article in Journal/Newspaper |
author |
Ndiana, Linda A Lanave, Gianvito Vasinioti, Violetta Desario, Costantina Martino, Camillo Colaianni, Maria Loredana Pellegrini, Francesco Camarda, Antonio Berjaoui, Shadia Sgroi, Giovanni Elia, Gabriella Pratelli, Annamaria Buono, Francesco Martella, Vito Buonavoglia, Canio Decaro, Nicola |
author_facet |
Ndiana, Linda A Lanave, Gianvito Vasinioti, Violetta Desario, Costantina Martino, Camillo Colaianni, Maria Loredana Pellegrini, Francesco Camarda, Antonio Berjaoui, Shadia Sgroi, Giovanni Elia, Gabriella Pratelli, Annamaria Buono, Francesco Martella, Vito Buonavoglia, Canio Decaro, Nicola |
author_sort |
Ndiana, Linda A |
title |
Detection and Genetic Characterization of Canine Adenoviruses, Circoviruses, and Novel Cycloviruses From Wild Carnivores in Italy |
title_short |
Detection and Genetic Characterization of Canine Adenoviruses, Circoviruses, and Novel Cycloviruses From Wild Carnivores in Italy |
title_full |
Detection and Genetic Characterization of Canine Adenoviruses, Circoviruses, and Novel Cycloviruses From Wild Carnivores in Italy |
title_fullStr |
Detection and Genetic Characterization of Canine Adenoviruses, Circoviruses, and Novel Cycloviruses From Wild Carnivores in Italy |
title_full_unstemmed |
Detection and Genetic Characterization of Canine Adenoviruses, Circoviruses, and Novel Cycloviruses From Wild Carnivores in Italy |
title_sort |
detection and genetic characterization of canine adenoviruses, circoviruses, and novel cycloviruses from wild carnivores in italy |
publishDate |
2022 |
url |
https://hdl.handle.net/11588/943423 https://doi.org/10.3389/fvets.2022.851987 |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000787912300001 volume:9 firstpage:851987 journal:FRONTIERS IN VETERINARY SCIENCE https://hdl.handle.net/11588/943423 doi:10.3389/fvets.2022.851987 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85128700868 |
op_doi |
https://doi.org/10.3389/fvets.2022.851987 |
container_title |
Frontiers in Veterinary Science |
container_volume |
9 |
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1809905115598946304 |