Bioactivity Screening of Antarctic Sponges Reveals Anticancer Activity and Potential Cell Death via Ferroptosis by Mycalols
Sponges are known to produce a series of compounds with bioactivities useful for human health. This study was conducted on four sponges collected in the framework of the XXXIV Italian National Antarctic Research Program (PNRA) in November-December 2018, i.e., Mycale (Oxymycale) acerata, Haliclona (R...
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Online Access: | https://hdl.handle.net/11588/902341 https://doi.org/10.3390/md19080459 |
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ftunivnapoliiris:oai:www.iris.unina.it:11588/902341 2024-09-09T19:05:47+00:00 Bioactivity Screening of Antarctic Sponges Reveals Anticancer Activity and Potential Cell Death via Ferroptosis by Mycalols Riccio, Gennaro Nuzzo, Genoveffa Zazo, Gianluca Coppola, Daniela Senese, Giuseppina Romano, Lucia Costantini, Maria Ruocco, Nadia Bertolino, Marco Fontana, Angelo Ianora, Adrianna Verde, Cinzia Giordano, Daniela Lauritano, Chiara Riccio, Gennaro Nuzzo, Genoveffa Zazo, Gianluca Coppola, Daniela Senese, Giuseppina Romano, Lucia Costantini, Maria Ruocco, Nadia Bertolino, Marco Fontana, Angelo Ianora, Adrianna Verde, Cinzia Giordano, Daniela Lauritano, Chiara 2021 https://hdl.handle.net/11588/902341 https://doi.org/10.3390/md19080459 eng eng info:eu-repo/semantics/altIdentifier/wos/WOS:000689361300001 volume:19 issue:8 firstpage:459 journal:MARINE DRUGS https://hdl.handle.net/11588/902341 doi:10.3390/md19080459 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85114372132 Antarctica drug discovery marine biotechnology mycalol sponge Animal Antarctic Region Antineoplastic Agent Aquatic Organism Cell Line Tumor Fatty Alcohol Hep G2 Cell Human Phytotherapy Porifera info:eu-repo/semantics/article 2021 ftunivnapoliiris https://doi.org/10.3390/md19080459 2024-06-17T15:19:21Z Sponges are known to produce a series of compounds with bioactivities useful for human health. This study was conducted on four sponges collected in the framework of the XXXIV Italian National Antarctic Research Program (PNRA) in November-December 2018, i.e., Mycale (Oxymycale) acerata, Haliclona (Rhizoniera) dancoi, Hemimycale topsenti, and Hemigellius pilosus. Sponge extracts were fractioned and tested against hepatocellular carcinoma (HepG2), lung carcinoma (A549), and melanoma cells (A2058), in order to screen for antiproliferative or cytotoxic activity. Two different chemical classes of compounds, belonging to mycalols and suberitenones, were identified in the active fractions. Mycalols were the most active compounds, and their mechanism of action was also investigated at the gene and protein levels in HepG2 cells. Of the differentially expressed genes, ULK1 and GALNT5 were the most down-regulated genes, while MAPK8 was one of the most up-regulated genes. These genes were previously associated with ferroptosis, a programmed cell death triggered by iron-dependent lipid peroxidation, confirmed at the protein level by the down-regulation of GPX4, a key regulator of ferroptosis, and the up-regulation of NCOA4, involved in iron homeostasis. These data suggest, for the first time, that mycalols act by triggering ferroptosis in HepG2 cells. Article in Journal/Newspaper Antarc* Antarctic Antarctica Italian National Antarctic Research Program National Antarctic Research Program IRIS Università degli Studi di Napoli Federico II Antarctic Marine Drugs 19 8 459 |
institution |
Open Polar |
collection |
IRIS Università degli Studi di Napoli Federico II |
op_collection_id |
ftunivnapoliiris |
language |
English |
topic |
Antarctica drug discovery marine biotechnology mycalol sponge Animal Antarctic Region Antineoplastic Agent Aquatic Organism Cell Line Tumor Fatty Alcohol Hep G2 Cell Human Phytotherapy Porifera |
spellingShingle |
Antarctica drug discovery marine biotechnology mycalol sponge Animal Antarctic Region Antineoplastic Agent Aquatic Organism Cell Line Tumor Fatty Alcohol Hep G2 Cell Human Phytotherapy Porifera Riccio, Gennaro Nuzzo, Genoveffa Zazo, Gianluca Coppola, Daniela Senese, Giuseppina Romano, Lucia Costantini, Maria Ruocco, Nadia Bertolino, Marco Fontana, Angelo Ianora, Adrianna Verde, Cinzia Giordano, Daniela Lauritano, Chiara Bioactivity Screening of Antarctic Sponges Reveals Anticancer Activity and Potential Cell Death via Ferroptosis by Mycalols |
topic_facet |
Antarctica drug discovery marine biotechnology mycalol sponge Animal Antarctic Region Antineoplastic Agent Aquatic Organism Cell Line Tumor Fatty Alcohol Hep G2 Cell Human Phytotherapy Porifera |
description |
Sponges are known to produce a series of compounds with bioactivities useful for human health. This study was conducted on four sponges collected in the framework of the XXXIV Italian National Antarctic Research Program (PNRA) in November-December 2018, i.e., Mycale (Oxymycale) acerata, Haliclona (Rhizoniera) dancoi, Hemimycale topsenti, and Hemigellius pilosus. Sponge extracts were fractioned and tested against hepatocellular carcinoma (HepG2), lung carcinoma (A549), and melanoma cells (A2058), in order to screen for antiproliferative or cytotoxic activity. Two different chemical classes of compounds, belonging to mycalols and suberitenones, were identified in the active fractions. Mycalols were the most active compounds, and their mechanism of action was also investigated at the gene and protein levels in HepG2 cells. Of the differentially expressed genes, ULK1 and GALNT5 were the most down-regulated genes, while MAPK8 was one of the most up-regulated genes. These genes were previously associated with ferroptosis, a programmed cell death triggered by iron-dependent lipid peroxidation, confirmed at the protein level by the down-regulation of GPX4, a key regulator of ferroptosis, and the up-regulation of NCOA4, involved in iron homeostasis. These data suggest, for the first time, that mycalols act by triggering ferroptosis in HepG2 cells. |
author2 |
Riccio, Gennaro Nuzzo, Genoveffa Zazo, Gianluca Coppola, Daniela Senese, Giuseppina Romano, Lucia Costantini, Maria Ruocco, Nadia Bertolino, Marco Fontana, Angelo Ianora, Adrianna Verde, Cinzia Giordano, Daniela Lauritano, Chiara |
format |
Article in Journal/Newspaper |
author |
Riccio, Gennaro Nuzzo, Genoveffa Zazo, Gianluca Coppola, Daniela Senese, Giuseppina Romano, Lucia Costantini, Maria Ruocco, Nadia Bertolino, Marco Fontana, Angelo Ianora, Adrianna Verde, Cinzia Giordano, Daniela Lauritano, Chiara |
author_facet |
Riccio, Gennaro Nuzzo, Genoveffa Zazo, Gianluca Coppola, Daniela Senese, Giuseppina Romano, Lucia Costantini, Maria Ruocco, Nadia Bertolino, Marco Fontana, Angelo Ianora, Adrianna Verde, Cinzia Giordano, Daniela Lauritano, Chiara |
author_sort |
Riccio, Gennaro |
title |
Bioactivity Screening of Antarctic Sponges Reveals Anticancer Activity and Potential Cell Death via Ferroptosis by Mycalols |
title_short |
Bioactivity Screening of Antarctic Sponges Reveals Anticancer Activity and Potential Cell Death via Ferroptosis by Mycalols |
title_full |
Bioactivity Screening of Antarctic Sponges Reveals Anticancer Activity and Potential Cell Death via Ferroptosis by Mycalols |
title_fullStr |
Bioactivity Screening of Antarctic Sponges Reveals Anticancer Activity and Potential Cell Death via Ferroptosis by Mycalols |
title_full_unstemmed |
Bioactivity Screening of Antarctic Sponges Reveals Anticancer Activity and Potential Cell Death via Ferroptosis by Mycalols |
title_sort |
bioactivity screening of antarctic sponges reveals anticancer activity and potential cell death via ferroptosis by mycalols |
publishDate |
2021 |
url |
https://hdl.handle.net/11588/902341 https://doi.org/10.3390/md19080459 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctica Italian National Antarctic Research Program National Antarctic Research Program |
genre_facet |
Antarc* Antarctic Antarctica Italian National Antarctic Research Program National Antarctic Research Program |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000689361300001 volume:19 issue:8 firstpage:459 journal:MARINE DRUGS https://hdl.handle.net/11588/902341 doi:10.3390/md19080459 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85114372132 |
op_doi |
https://doi.org/10.3390/md19080459 |
container_title |
Marine Drugs |
container_volume |
19 |
container_issue |
8 |
container_start_page |
459 |
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1809819784117747712 |