Molecular response of Sargassum vulgare to acidification at volcanic CO2 vents: Insights from proteomic and metabolite analyses
Ocean acidification is impacting marine life all over the world. Understanding how species can cope with the changes in seawater carbonate chemistry represents a challenging issue. We addressed this topic using underwater CO2 vents that naturally acidify some marine areas off the island of Ischia. I...
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Online Access: | https://hdl.handle.net/11588/899488 https://doi.org/10.1111/mec.16553 |
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ftunivnapoliiris:oai:www.iris.unina.it:11588/899488 2024-04-14T08:17:39+00:00 Molecular response of Sargassum vulgare to acidification at volcanic CO2 vents: Insights from proteomic and metabolite analyses Kumar, Amit Nonnis, Simona Castellano, Immacolata AbdElgawad, Hamada Beemster, Gerrit T S Buia, Maria Cristina Maffioli, Elisa Tedeschi, Gabriella Palumbo, Anna Kumar, Amit Nonnis, Simona Castellano, Immacolata Abdelgawad, Hamada Beemster, Gerrit T S Buia, Maria Cristina Maffioli, Elisa Tedeschi, Gabriella Palumbo, Anna 2022 https://hdl.handle.net/11588/899488 https://doi.org/10.1111/mec.16553 eng eng info:eu-repo/semantics/altIdentifier/wos/WOS:000812867100001 volume:31 issue:14 firstpage:3844 lastpage:3858 numberofpages:15 journal:MOLECULAR ECOLOGY https://hdl.handle.net/11588/899488 doi:10.1111/mec.16553 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85132327033 CO2 seep adaptation macroalgae metabolite ocean acidification protein Carbon Dioxide Hydrogen-Ion Concentration Proteomic Seawater Sargassum info:eu-repo/semantics/article 2022 ftunivnapoliiris https://doi.org/10.1111/mec.16553 2024-03-21T18:46:55Z Ocean acidification is impacting marine life all over the world. Understanding how species can cope with the changes in seawater carbonate chemistry represents a challenging issue. We addressed this topic using underwater CO2 vents that naturally acidify some marine areas off the island of Ischia. In the most acidified area of the vents, having a mean pH value of 6.7, comparable to far-future predicted acidification scenarios (by 2300), the biomass is dominated by the brown alga Sargassum vulgare. The novelty of the present study is the characterization of the S. vulgare proteome together with metabolite analyses to identify the key proteins, metabolites, and pathways affected by ocean acidification. A total of 367 and 387 proteins were identified in populations grown at pH that approximates the current global average (8.1) and acidified sites, respectively. Analysis of their relative abundance revealed that 304 proteins are present in samples from both sites: 111 proteins are either higher or exclusively present under acidified conditions, whereas 120 proteins are either lower or present only under control conditions. Functionally, under acidification, a decrease in proteins related to translation and post-translational processes and an increase of proteins involved in photosynthesis, glycolysis, oxidation-reduction processes, and protein folding were observed. In addition, small-molecule metabolism was affected, leading to a decrease of some fatty acids and antioxidant compounds under acidification. Overall, the results obtained by proteins and metabolites analyses, integrated with previous transcriptomic, physiological, and biochemical studies, allowed us to delineate the molecular strategies adopted by S. vulgare to grow in future acidified environments, including an increase of proteins involved in energetic metabolism, oxidation-reduction processes, and protein folding at the expense of proteins involved in translation and post-translational processes. Article in Journal/Newspaper Ocean acidification IRIS Università degli Studi di Napoli Federico II Molecular Ecology 31 14 3844 3858 |
institution |
Open Polar |
collection |
IRIS Università degli Studi di Napoli Federico II |
op_collection_id |
ftunivnapoliiris |
language |
English |
topic |
CO2 seep adaptation macroalgae metabolite ocean acidification protein Carbon Dioxide Hydrogen-Ion Concentration Proteomic Seawater Sargassum |
spellingShingle |
CO2 seep adaptation macroalgae metabolite ocean acidification protein Carbon Dioxide Hydrogen-Ion Concentration Proteomic Seawater Sargassum Kumar, Amit Nonnis, Simona Castellano, Immacolata AbdElgawad, Hamada Beemster, Gerrit T S Buia, Maria Cristina Maffioli, Elisa Tedeschi, Gabriella Palumbo, Anna Molecular response of Sargassum vulgare to acidification at volcanic CO2 vents: Insights from proteomic and metabolite analyses |
topic_facet |
CO2 seep adaptation macroalgae metabolite ocean acidification protein Carbon Dioxide Hydrogen-Ion Concentration Proteomic Seawater Sargassum |
description |
Ocean acidification is impacting marine life all over the world. Understanding how species can cope with the changes in seawater carbonate chemistry represents a challenging issue. We addressed this topic using underwater CO2 vents that naturally acidify some marine areas off the island of Ischia. In the most acidified area of the vents, having a mean pH value of 6.7, comparable to far-future predicted acidification scenarios (by 2300), the biomass is dominated by the brown alga Sargassum vulgare. The novelty of the present study is the characterization of the S. vulgare proteome together with metabolite analyses to identify the key proteins, metabolites, and pathways affected by ocean acidification. A total of 367 and 387 proteins were identified in populations grown at pH that approximates the current global average (8.1) and acidified sites, respectively. Analysis of their relative abundance revealed that 304 proteins are present in samples from both sites: 111 proteins are either higher or exclusively present under acidified conditions, whereas 120 proteins are either lower or present only under control conditions. Functionally, under acidification, a decrease in proteins related to translation and post-translational processes and an increase of proteins involved in photosynthesis, glycolysis, oxidation-reduction processes, and protein folding were observed. In addition, small-molecule metabolism was affected, leading to a decrease of some fatty acids and antioxidant compounds under acidification. Overall, the results obtained by proteins and metabolites analyses, integrated with previous transcriptomic, physiological, and biochemical studies, allowed us to delineate the molecular strategies adopted by S. vulgare to grow in future acidified environments, including an increase of proteins involved in energetic metabolism, oxidation-reduction processes, and protein folding at the expense of proteins involved in translation and post-translational processes. |
author2 |
Kumar, Amit Nonnis, Simona Castellano, Immacolata Abdelgawad, Hamada Beemster, Gerrit T S Buia, Maria Cristina Maffioli, Elisa Tedeschi, Gabriella Palumbo, Anna |
format |
Article in Journal/Newspaper |
author |
Kumar, Amit Nonnis, Simona Castellano, Immacolata AbdElgawad, Hamada Beemster, Gerrit T S Buia, Maria Cristina Maffioli, Elisa Tedeschi, Gabriella Palumbo, Anna |
author_facet |
Kumar, Amit Nonnis, Simona Castellano, Immacolata AbdElgawad, Hamada Beemster, Gerrit T S Buia, Maria Cristina Maffioli, Elisa Tedeschi, Gabriella Palumbo, Anna |
author_sort |
Kumar, Amit |
title |
Molecular response of Sargassum vulgare to acidification at volcanic CO2 vents: Insights from proteomic and metabolite analyses |
title_short |
Molecular response of Sargassum vulgare to acidification at volcanic CO2 vents: Insights from proteomic and metabolite analyses |
title_full |
Molecular response of Sargassum vulgare to acidification at volcanic CO2 vents: Insights from proteomic and metabolite analyses |
title_fullStr |
Molecular response of Sargassum vulgare to acidification at volcanic CO2 vents: Insights from proteomic and metabolite analyses |
title_full_unstemmed |
Molecular response of Sargassum vulgare to acidification at volcanic CO2 vents: Insights from proteomic and metabolite analyses |
title_sort |
molecular response of sargassum vulgare to acidification at volcanic co2 vents: insights from proteomic and metabolite analyses |
publishDate |
2022 |
url |
https://hdl.handle.net/11588/899488 https://doi.org/10.1111/mec.16553 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000812867100001 volume:31 issue:14 firstpage:3844 lastpage:3858 numberofpages:15 journal:MOLECULAR ECOLOGY https://hdl.handle.net/11588/899488 doi:10.1111/mec.16553 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85132327033 |
op_doi |
https://doi.org/10.1111/mec.16553 |
container_title |
Molecular Ecology |
container_volume |
31 |
container_issue |
14 |
container_start_page |
3844 |
op_container_end_page |
3858 |
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1796316885482995712 |