Biomolecular Condensates under Extreme Martian Salt Conditions
Biomolecular condensates formed by liquid-liquid phase separation (LLPS) are considered one of the early compartmentalization strategies of cells, which still prevail today forming nonmembranous compartments in biological cells. Studies of the effect of high pressures, such as those encountered in t...
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ftunivnapoliiris:oai:www.iris.unina.it:11588/892865 2024-01-28T10:00:38+01:00 Biomolecular Condensates under Extreme Martian Salt Conditions Fetahaj, Zamira Ostermeier, Lena Cinar, Hasan Oliva, Rosario Winter, Roland Fetahaj, Zamira Ostermeier, Lena Cinar, Hasan Oliva, Rosario Winter, Roland 2021 http://hdl.handle.net/11588/892865 https://doi.org/10.1021/jacs.1c01832 eng eng info:eu-repo/semantics/altIdentifier/wos/WOS:000639019400045 volume:143 issue:13 firstpage:5247 lastpage:5259 numberofpages:13 journal:JOURNAL OF THE AMERICAN CHEMICAL SOCIETY http://hdl.handle.net/11588/892865 doi:10.1021/jacs.1c01832 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85104046449 Amino Acid Sequence Extraterrestrial Environment Hydrophobic and Hydrophilic Interaction Liquid-Liquid Extraction Mar Protein Salinity Salt Temperature info:eu-repo/semantics/article 2021 ftunivnapoliiris https://doi.org/10.1021/jacs.1c01832 2024-01-03T00:04:10Z Biomolecular condensates formed by liquid-liquid phase separation (LLPS) are considered one of the early compartmentalization strategies of cells, which still prevail today forming nonmembranous compartments in biological cells. Studies of the effect of high pressures, such as those encountered in the subsurface salt lakes of Mars or in the depths of the subseafloor on Earth, on biomolecular LLPS will contribute to questions of protocell formation under prebiotic conditions. We investigated the effects of extreme environmental conditions, focusing on highly aggressive Martian salts (perchlorate and sulfate) and high pressure, on the formation of biomolecular condensates of proteins. Our data show that the driving force for phase separation of proteins is not only sensitively dictated by their amino acid sequence but also strongly influenced by the type of salt and its concentration. At high salinity, as encountered in Martian soil and similar harsh environments on Earth, attractive short-range interactions, ion correlation effects, hydrophobic, and π-driven interactions can sustain LLPS for suitable polypeptide sequences. Our results also show that salts across the Hofmeister series have a differential effect on shifting the boundary of immiscibility that determines phase separation. In addition, we show that confinement mimicking cracks in sediments and subsurface saline water pools in the Antarctica or on Mars can dramatically stabilize liquid phase droplets, leading to an increase in the temperature and pressure stability of the droplet phase. Article in Journal/Newspaper Antarc* Antarctica IRIS Università degli Studi di Napoli Federico II Journal of the American Chemical Society 143 13 5247 5259 |
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Open Polar |
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IRIS Università degli Studi di Napoli Federico II |
op_collection_id |
ftunivnapoliiris |
language |
English |
topic |
Amino Acid Sequence Extraterrestrial Environment Hydrophobic and Hydrophilic Interaction Liquid-Liquid Extraction Mar Protein Salinity Salt Temperature |
spellingShingle |
Amino Acid Sequence Extraterrestrial Environment Hydrophobic and Hydrophilic Interaction Liquid-Liquid Extraction Mar Protein Salinity Salt Temperature Fetahaj, Zamira Ostermeier, Lena Cinar, Hasan Oliva, Rosario Winter, Roland Biomolecular Condensates under Extreme Martian Salt Conditions |
topic_facet |
Amino Acid Sequence Extraterrestrial Environment Hydrophobic and Hydrophilic Interaction Liquid-Liquid Extraction Mar Protein Salinity Salt Temperature |
description |
Biomolecular condensates formed by liquid-liquid phase separation (LLPS) are considered one of the early compartmentalization strategies of cells, which still prevail today forming nonmembranous compartments in biological cells. Studies of the effect of high pressures, such as those encountered in the subsurface salt lakes of Mars or in the depths of the subseafloor on Earth, on biomolecular LLPS will contribute to questions of protocell formation under prebiotic conditions. We investigated the effects of extreme environmental conditions, focusing on highly aggressive Martian salts (perchlorate and sulfate) and high pressure, on the formation of biomolecular condensates of proteins. Our data show that the driving force for phase separation of proteins is not only sensitively dictated by their amino acid sequence but also strongly influenced by the type of salt and its concentration. At high salinity, as encountered in Martian soil and similar harsh environments on Earth, attractive short-range interactions, ion correlation effects, hydrophobic, and π-driven interactions can sustain LLPS for suitable polypeptide sequences. Our results also show that salts across the Hofmeister series have a differential effect on shifting the boundary of immiscibility that determines phase separation. In addition, we show that confinement mimicking cracks in sediments and subsurface saline water pools in the Antarctica or on Mars can dramatically stabilize liquid phase droplets, leading to an increase in the temperature and pressure stability of the droplet phase. |
author2 |
Fetahaj, Zamira Ostermeier, Lena Cinar, Hasan Oliva, Rosario Winter, Roland |
format |
Article in Journal/Newspaper |
author |
Fetahaj, Zamira Ostermeier, Lena Cinar, Hasan Oliva, Rosario Winter, Roland |
author_facet |
Fetahaj, Zamira Ostermeier, Lena Cinar, Hasan Oliva, Rosario Winter, Roland |
author_sort |
Fetahaj, Zamira |
title |
Biomolecular Condensates under Extreme Martian Salt Conditions |
title_short |
Biomolecular Condensates under Extreme Martian Salt Conditions |
title_full |
Biomolecular Condensates under Extreme Martian Salt Conditions |
title_fullStr |
Biomolecular Condensates under Extreme Martian Salt Conditions |
title_full_unstemmed |
Biomolecular Condensates under Extreme Martian Salt Conditions |
title_sort |
biomolecular condensates under extreme martian salt conditions |
publishDate |
2021 |
url |
http://hdl.handle.net/11588/892865 https://doi.org/10.1021/jacs.1c01832 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000639019400045 volume:143 issue:13 firstpage:5247 lastpage:5259 numberofpages:13 journal:JOURNAL OF THE AMERICAN CHEMICAL SOCIETY http://hdl.handle.net/11588/892865 doi:10.1021/jacs.1c01832 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85104046449 |
op_doi |
https://doi.org/10.1021/jacs.1c01832 |
container_title |
Journal of the American Chemical Society |
container_volume |
143 |
container_issue |
13 |
container_start_page |
5247 |
op_container_end_page |
5259 |
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