A physico-chemical approach to understanding the structure, the conformation, and the activity of mannan polysaccharides.
Extracellular polysaccharides are widely produced by bacteria, yeasts, and algae. These polymers are involved in several biological functions, such as bacteria adhesion to surface and biofilm formation, ion sequestering, protection from desiccation, and cryoprotection. The chemical characterization...
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ftunivnapoliiris:oai:www.iris.unina.it:11588/852303 2024-09-09T19:44:36+00:00 A physico-chemical approach to understanding the structure, the conformation, and the activity of mannan polysaccharides. Angela Casillo Antonio Fabozzi Irene Russo-Krauss Ermenegilda Parrilli Caroline I. Biggs Matthew I. Gibson Rosa Lanzetta Marie-Sousay Appavou Aurel Radulescu Maria Luisa Tutino Luigi Paduano Maria Michela Corsaro Casillo, Angela Fabozzi, Antonio Russo-Krauss, Irene Parrilli, Ermenegilda Biggs, Caroline I. Gibson, Matthew I. Lanzetta, Rosa Appavou, Marie-Sousay Radulescu, Aurel Tutino, MARIA LUISA Paduano, Luigi Corsaro, MARIA MICHELA 2021 https://hdl.handle.net/11588/852303 https://doi.org/10.1021/acs.biomac.0c01659 eng eng info:eu-repo/semantics/altIdentifier/wos/WOS:000640310700010 volume:22 firstpage:1445 lastpage:1457 numberofpages:13 journal:BIOMACROMOLECULES https://hdl.handle.net/11588/852303 doi:10.1021/acs.biomac.0c01659 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85103831882 info:eu-repo/semantics/article 2021 ftunivnapoliiris https://doi.org/10.1021/acs.biomac.0c01659 2024-06-17T15:19:35Z Extracellular polysaccharides are widely produced by bacteria, yeasts, and algae. These polymers are involved in several biological functions, such as bacteria adhesion to surface and biofilm formation, ion sequestering, protection from desiccation, and cryoprotection. The chemical characterization of these polymers is the starting point for obtaining relationships between their structures and their various functions. While this fundamental correlation is well reported and studied for the proteins, for the polysaccharides, this relationship is less intuitive. In this paper, we elucidate the chemical structure and conformational studies of a mannan exopolysaccharide from the permafrost isolated bacterium Psychrobacter arcticus strain 273-4. The mannan from the cold-adapted bacterium was compared with its dephosphorylated derivative and the commercial product from Saccharomyces cerevisiae. Starting from the chemical structure, we explored a new approach to deepen the study of the structure/activity relationship. A pool of physicochemical techniques, ranging from small-angle neutron scattering (SANS) and dynamic and static light scattering (DLS and SLS respectively) to circular dichroism (CD) and cryo-transmission electron microscopy (cryo-TEM), have been used. Finally, the ice recrystallization inhibition activity of the polysaccharides was explored. The experimental evidence suggests that the mannan exopolysaccharide from P. arcticus bacterium has an efficient interaction with the water molecules, and it is structurally characterized by rigid-rod regions assuming a 14-helix-type conformation. Article in Journal/Newspaper Ice permafrost IRIS Università degli Studi di Napoli Federico II Biomacromolecules 22 4 1445 1457 |
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IRIS Università degli Studi di Napoli Federico II |
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ftunivnapoliiris |
language |
English |
description |
Extracellular polysaccharides are widely produced by bacteria, yeasts, and algae. These polymers are involved in several biological functions, such as bacteria adhesion to surface and biofilm formation, ion sequestering, protection from desiccation, and cryoprotection. The chemical characterization of these polymers is the starting point for obtaining relationships between their structures and their various functions. While this fundamental correlation is well reported and studied for the proteins, for the polysaccharides, this relationship is less intuitive. In this paper, we elucidate the chemical structure and conformational studies of a mannan exopolysaccharide from the permafrost isolated bacterium Psychrobacter arcticus strain 273-4. The mannan from the cold-adapted bacterium was compared with its dephosphorylated derivative and the commercial product from Saccharomyces cerevisiae. Starting from the chemical structure, we explored a new approach to deepen the study of the structure/activity relationship. A pool of physicochemical techniques, ranging from small-angle neutron scattering (SANS) and dynamic and static light scattering (DLS and SLS respectively) to circular dichroism (CD) and cryo-transmission electron microscopy (cryo-TEM), have been used. Finally, the ice recrystallization inhibition activity of the polysaccharides was explored. The experimental evidence suggests that the mannan exopolysaccharide from P. arcticus bacterium has an efficient interaction with the water molecules, and it is structurally characterized by rigid-rod regions assuming a 14-helix-type conformation. |
author2 |
Casillo, Angela Fabozzi, Antonio Russo-Krauss, Irene Parrilli, Ermenegilda Biggs, Caroline I. Gibson, Matthew I. Lanzetta, Rosa Appavou, Marie-Sousay Radulescu, Aurel Tutino, MARIA LUISA Paduano, Luigi Corsaro, MARIA MICHELA |
format |
Article in Journal/Newspaper |
author |
Angela Casillo Antonio Fabozzi Irene Russo-Krauss Ermenegilda Parrilli Caroline I. Biggs Matthew I. Gibson Rosa Lanzetta Marie-Sousay Appavou Aurel Radulescu Maria Luisa Tutino Luigi Paduano Maria Michela Corsaro |
spellingShingle |
Angela Casillo Antonio Fabozzi Irene Russo-Krauss Ermenegilda Parrilli Caroline I. Biggs Matthew I. Gibson Rosa Lanzetta Marie-Sousay Appavou Aurel Radulescu Maria Luisa Tutino Luigi Paduano Maria Michela Corsaro A physico-chemical approach to understanding the structure, the conformation, and the activity of mannan polysaccharides. |
author_facet |
Angela Casillo Antonio Fabozzi Irene Russo-Krauss Ermenegilda Parrilli Caroline I. Biggs Matthew I. Gibson Rosa Lanzetta Marie-Sousay Appavou Aurel Radulescu Maria Luisa Tutino Luigi Paduano Maria Michela Corsaro |
author_sort |
Angela Casillo |
title |
A physico-chemical approach to understanding the structure, the conformation, and the activity of mannan polysaccharides. |
title_short |
A physico-chemical approach to understanding the structure, the conformation, and the activity of mannan polysaccharides. |
title_full |
A physico-chemical approach to understanding the structure, the conformation, and the activity of mannan polysaccharides. |
title_fullStr |
A physico-chemical approach to understanding the structure, the conformation, and the activity of mannan polysaccharides. |
title_full_unstemmed |
A physico-chemical approach to understanding the structure, the conformation, and the activity of mannan polysaccharides. |
title_sort |
physico-chemical approach to understanding the structure, the conformation, and the activity of mannan polysaccharides. |
publishDate |
2021 |
url |
https://hdl.handle.net/11588/852303 https://doi.org/10.1021/acs.biomac.0c01659 |
genre |
Ice permafrost |
genre_facet |
Ice permafrost |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000640310700010 volume:22 firstpage:1445 lastpage:1457 numberofpages:13 journal:BIOMACROMOLECULES https://hdl.handle.net/11588/852303 doi:10.1021/acs.biomac.0c01659 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85103831882 |
op_doi |
https://doi.org/10.1021/acs.biomac.0c01659 |
container_title |
Biomacromolecules |
container_volume |
22 |
container_issue |
4 |
container_start_page |
1445 |
op_container_end_page |
1457 |
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1809914274131214336 |