Living in future ocean acidification, physiological adaptive responses of the immune system of sea urchins resident at a CO 2 vent system
The effects of ocean acidification, a major anthropogenic impact on marine life, have been mainly investigated in laboratory/mesocosm experiments. We used the CO 2 vents at Ischia as a natural laboratory to study the long-term effects of ocean acidification on the sea urchin Paracentrotus lividus po...
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Online Access: | http://hdl.handle.net/11588/810428 https://doi.org/10.1016/j.scitotenv.2019.04.005 |
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ftunivnapoliiris:oai:www.iris.unina.it:11588/810428 2024-02-11T10:07:21+01:00 Living in future ocean acidification, physiological adaptive responses of the immune system of sea urchins resident at a CO 2 vent system Migliaccio O. Pinsino A. Maffioli E. Smith A. M. Agnisola C. Matranga V. Nonnis S. Tedeschi G. Byrne M. Gambi M. C. Palumbo A. Migliaccio, O. Pinsino, A. Maffioli, E. Smith, A. M. Agnisola, C. Matranga, V. Nonnis, S. Tedeschi, G. Byrne, M. Gambi, M. C. Palumbo, A. 2019 http://hdl.handle.net/11588/810428 https://doi.org/10.1016/j.scitotenv.2019.04.005 eng eng info:eu-repo/semantics/altIdentifier/wos/WOS:000466979400087 volume:672 firstpage:938 lastpage:950 numberofpages:13 journal:SCIENCE OF THE TOTAL ENVIRONMENT http://hdl.handle.net/11588/810428 doi:10.1016/j.scitotenv.2019.04.005 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85064086391 info:eu-repo/semantics/restrictedAccess Adaptation Eco-physiology Immune cell Ocean acidification Proteomic Sea urchin Physiological Animal Carbon Dioxide Environmental Monitoring Hydrogen-Ion Concentration Hydrothermal Vent Immune System Paracentrotu Seawater Water Pollutants Chemical info:eu-repo/semantics/article 2019 ftunivnapoliiris https://doi.org/10.1016/j.scitotenv.2019.04.005 2024-01-16T23:34:09Z The effects of ocean acidification, a major anthropogenic impact on marine life, have been mainly investigated in laboratory/mesocosm experiments. We used the CO 2 vents at Ischia as a natural laboratory to study the long-term effects of ocean acidification on the sea urchin Paracentrotus lividus population resident in low-pH (7.8 ± 0.2) compared to that at two control sites (pH 8.02 ± 0.00; 8.02 ± 0.01). The novelty of the present study is the analysis of the sea urchin immune cells, the sentinels of environmental stress responses, by a wide-ranging approach, including cell morphology, biochemistry and proteomics. Immune cell proteomics showed that 311 proteins were differentially expressed in urchins across sites with a general shift towards antioxidant processes in the vent urchins. The vent urchin immune cells showed higher levels of total antioxidant capacity, up-regulation of phagosome and microsomal proteins, enzymes of ammonium metabolism, amino-acid degradation, and modulation of carbon metabolism proteins. Lipid-hydroperoxides and nitric oxide levels were not different in urchins from the different sites. No differences in the coelomic fluid pH, immune cell composition, animal respiration, nitrogen excretion and skeletal mineralogy were observed. Our results reveal the phenotypic plasticity of the immune system of sea urchins adapted to life at vent site, under conditions commensurate with near-future ocean acidification projections. Article in Journal/Newspaper Ocean acidification IRIS Università degli Studi di Napoli Federico II The Sentinels ENVELOPE(-36.267,-36.267,-54.267,-54.267) Science of The Total Environment 672 938 950 |
institution |
Open Polar |
collection |
IRIS Università degli Studi di Napoli Federico II |
op_collection_id |
ftunivnapoliiris |
language |
English |
topic |
Adaptation Eco-physiology Immune cell Ocean acidification Proteomic Sea urchin Physiological Animal Carbon Dioxide Environmental Monitoring Hydrogen-Ion Concentration Hydrothermal Vent Immune System Paracentrotu Seawater Water Pollutants Chemical |
spellingShingle |
Adaptation Eco-physiology Immune cell Ocean acidification Proteomic Sea urchin Physiological Animal Carbon Dioxide Environmental Monitoring Hydrogen-Ion Concentration Hydrothermal Vent Immune System Paracentrotu Seawater Water Pollutants Chemical Migliaccio O. Pinsino A. Maffioli E. Smith A. M. Agnisola C. Matranga V. Nonnis S. Tedeschi G. Byrne M. Gambi M. C. Palumbo A. Living in future ocean acidification, physiological adaptive responses of the immune system of sea urchins resident at a CO 2 vent system |
topic_facet |
Adaptation Eco-physiology Immune cell Ocean acidification Proteomic Sea urchin Physiological Animal Carbon Dioxide Environmental Monitoring Hydrogen-Ion Concentration Hydrothermal Vent Immune System Paracentrotu Seawater Water Pollutants Chemical |
description |
The effects of ocean acidification, a major anthropogenic impact on marine life, have been mainly investigated in laboratory/mesocosm experiments. We used the CO 2 vents at Ischia as a natural laboratory to study the long-term effects of ocean acidification on the sea urchin Paracentrotus lividus population resident in low-pH (7.8 ± 0.2) compared to that at two control sites (pH 8.02 ± 0.00; 8.02 ± 0.01). The novelty of the present study is the analysis of the sea urchin immune cells, the sentinels of environmental stress responses, by a wide-ranging approach, including cell morphology, biochemistry and proteomics. Immune cell proteomics showed that 311 proteins were differentially expressed in urchins across sites with a general shift towards antioxidant processes in the vent urchins. The vent urchin immune cells showed higher levels of total antioxidant capacity, up-regulation of phagosome and microsomal proteins, enzymes of ammonium metabolism, amino-acid degradation, and modulation of carbon metabolism proteins. Lipid-hydroperoxides and nitric oxide levels were not different in urchins from the different sites. No differences in the coelomic fluid pH, immune cell composition, animal respiration, nitrogen excretion and skeletal mineralogy were observed. Our results reveal the phenotypic plasticity of the immune system of sea urchins adapted to life at vent site, under conditions commensurate with near-future ocean acidification projections. |
author2 |
Migliaccio, O. Pinsino, A. Maffioli, E. Smith, A. M. Agnisola, C. Matranga, V. Nonnis, S. Tedeschi, G. Byrne, M. Gambi, M. C. Palumbo, A. |
format |
Article in Journal/Newspaper |
author |
Migliaccio O. Pinsino A. Maffioli E. Smith A. M. Agnisola C. Matranga V. Nonnis S. Tedeschi G. Byrne M. Gambi M. C. Palumbo A. |
author_facet |
Migliaccio O. Pinsino A. Maffioli E. Smith A. M. Agnisola C. Matranga V. Nonnis S. Tedeschi G. Byrne M. Gambi M. C. Palumbo A. |
author_sort |
Migliaccio O. |
title |
Living in future ocean acidification, physiological adaptive responses of the immune system of sea urchins resident at a CO 2 vent system |
title_short |
Living in future ocean acidification, physiological adaptive responses of the immune system of sea urchins resident at a CO 2 vent system |
title_full |
Living in future ocean acidification, physiological adaptive responses of the immune system of sea urchins resident at a CO 2 vent system |
title_fullStr |
Living in future ocean acidification, physiological adaptive responses of the immune system of sea urchins resident at a CO 2 vent system |
title_full_unstemmed |
Living in future ocean acidification, physiological adaptive responses of the immune system of sea urchins resident at a CO 2 vent system |
title_sort |
living in future ocean acidification, physiological adaptive responses of the immune system of sea urchins resident at a co 2 vent system |
publishDate |
2019 |
url |
http://hdl.handle.net/11588/810428 https://doi.org/10.1016/j.scitotenv.2019.04.005 |
long_lat |
ENVELOPE(-36.267,-36.267,-54.267,-54.267) |
geographic |
The Sentinels |
geographic_facet |
The Sentinels |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000466979400087 volume:672 firstpage:938 lastpage:950 numberofpages:13 journal:SCIENCE OF THE TOTAL ENVIRONMENT http://hdl.handle.net/11588/810428 doi:10.1016/j.scitotenv.2019.04.005 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85064086391 |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1016/j.scitotenv.2019.04.005 |
container_title |
Science of The Total Environment |
container_volume |
672 |
container_start_page |
938 |
op_container_end_page |
950 |
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1790605893093556224 |