MOLECULAR EFFECTS ON SPERMATOZOA OF MYTILUS GALLOPROVINCIALIS EXPOSED TO SEVERE HYPOSALINE CONDITIONS: A CASE OF FERTILITY PRESERVATION STRATEGY DUE TO GAMETE PLASTICITY.

• Main Authors: M. Piscopo, Gennaro Lettieri, Martina Maione, Maria Antonietta Ranauda, Elena Mele
• Other Authors: UNIVERSITA' DEGLI STUDI DI PADOVA, Piscopo, M., Lettieri, Gennaro, Maione, Martina, Antonietta Ranauda, Maria, Mele, Elena
• Format: Conference Object
• Language: English
• Published: UNIVERSITA' DEGLI STUDI DI PADOVA 2019
• Subjects: Mytilus galloprovinciali; hyposalinity; sperm chromatin; reproduction; protamine-like proteins; Pacific; North Atlantic; Ocean acidification
• Online Access: http://hdl.handle.net/11588/758407

Climate changes are affecting species physiology, pushing environmental tolerance limits and shifting biogeographic distribution ranges of marine organisms. In addition to temperature and ocean acidification, global climate changes can also occur through changes in seawater salinity. Salinity levels may change also by anthropogenic factors such as mining activity and agricultural and industrial processes. Salinity is considered one of the most significant environmental stressors for marine bivalves. Mussels, in close proximity to coasts and in estuaries, in fact, periodically experience hypo-saline stress, particularly during intense precipitations. Many bivalve species, when experiencing water with high temperatures and low salinity, have the skill to acclimatize, or migrate to deeper water which is cooler and more saline. Mytilus galloprovincialis, is distributed in the north Atlantic Ocean, Mediterranean Sea and Black Sea and in recent years has invaded new places, including South Africa, Japan and California. This mussel is a common member of the intertidal estuarine and coastal areas where it can meet different salinities. Mytilus galloprovincialis can survive at elevated heat exposure, but it is susceptible to hyposalinity, while the native Pacific coast M. trossulus is more tolerant to hypo-saline conditions but vulnerable to heat stress. Since salinity represents a critical factor in reproduction of marine species, we analyzed the responses of Mytilus galloprovincialis spermatozoa to hyposaline stress. We exposed mussels, in laboratory tanks, for 24 hours at 18°C to control (35.9 psu) and three hyposaline (17.1; 22.6 and 26.2 psu) conditions, and evaluated the expression of sperm hsp70 and protamine-like proteins genes. Further we analyzed the electrophoretic pattern, the DNA binding and the release from sperm nuclei of protamine-like proteins. For all experimental approaches used, the results obtained at 17.1 psu condition were very similar to those obtained in control condition, while alterations were ...