Retrospective study of pathology-based investigative techniques for the assessment of diet-induced changes in liver and intestine of flatfish
This article proposes a systematic evaluation of methods pertaining to the pathology discipline (histology, histochemistry, ultrastructure, in situ techniques, image analysis including morphom-etry and fractal dimension analysis) employed to study liver and intestine status during a series of nutrit...
Published in: | Italian Journal of Animal Science |
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Online Access: | http://hdl.handle.net/11588/702005 https://doi.org/10.1080/1828051X.2017.1364610 http://www.tandfonline.com/loi/tjas20#.VpSlwLfUhFo |
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ftunivnapoliiris:oai:www.iris.unina.it:11588/702005 2024-06-23T07:56:37+00:00 Retrospective study of pathology-based investigative techniques for the assessment of diet-induced changes in liver and intestine of flatfish Sirri, Rubina Sarli, Giuseppe Bianco, Carlo Bonaldo, Alessio Gatta, Pier Paolo Fontanillas, Ramon De Vico, Gionata Carella, Francesca Brachelente, Chiara Parma, Luca Mandrioli, Luciana Sirri, Rubina Sarli, Giuseppe Bianco, Carlo Bonaldo, Alessio Gatta, Pier Paolo Fontanillas, Ramon De Vico, Gionata Carella, Francesca Brachelente, Chiara Parma, Luca Mandrioli, Luciana 2018 http://hdl.handle.net/11588/702005 https://doi.org/10.1080/1828051X.2017.1364610 http://www.tandfonline.com/loi/tjas20#.VpSlwLfUhFo eng eng info:eu-repo/semantics/altIdentifier/wos/WOS:000424424400031 volume:17 issue:2 firstpage:518 lastpage:529 numberofpages:12 journal:ITALIAN JOURNAL OF ANIMAL SCIENCE http://hdl.handle.net/11588/702005 doi:10.1080/1828051X.2017.1364610 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85041555293 http://www.tandfonline.com/loi/tjas20#.VpSlwLfUhFo info:eu-repo/semantics/restrictedAccess diet Flatfish gut histology liver Animal Science and Zoology info:eu-repo/semantics/article 2018 ftunivnapoliiris https://doi.org/10.1080/1828051X.2017.1364610 2024-06-10T14:58:50Z This article proposes a systematic evaluation of methods pertaining to the pathology discipline (histology, histochemistry, ultrastructure, in situ techniques, image analysis including morphom-etry and fractal dimension analysis) employed to study liver and intestine status during a series of nutritional trials performed on flatfish species (common sole, Solea solea; Senegal sole, Solea senegalensis and turbot, Scophthalmus maximus) of commercial interest. Histology is the first step able to detect the normal architecture of the tissues or the possible occurring changes related to inflammation, degeneration and cell death. The evaluation of liver should include a scoring system to obtain semi-quantitative numerical data that are statistically analysable; histochemisty is a valid tool for identifying the type of cell content, together with ultrastructure that also permits the detection of even mild signs of cell damage. For the evaluation of the intestine, in situ techniques are useful together with fractal dimension analysis to determine the mucosal tro-phism, while ultrastructure can demonstrate cell injury. These considerations were resumed in a methodological flowchart as a valid tool to assess the fish health status when nutritional trials are planned. Article in Journal/Newspaper Scophthalmus maximus Turbot IRIS Università degli Studi di Napoli Federico II Italian Journal of Animal Science 17 2 518 529 |
institution |
Open Polar |
collection |
IRIS Università degli Studi di Napoli Federico II |
op_collection_id |
ftunivnapoliiris |
language |
English |
topic |
diet Flatfish gut histology liver Animal Science and Zoology |
spellingShingle |
diet Flatfish gut histology liver Animal Science and Zoology Sirri, Rubina Sarli, Giuseppe Bianco, Carlo Bonaldo, Alessio Gatta, Pier Paolo Fontanillas, Ramon De Vico, Gionata Carella, Francesca Brachelente, Chiara Parma, Luca Mandrioli, Luciana Retrospective study of pathology-based investigative techniques for the assessment of diet-induced changes in liver and intestine of flatfish |
topic_facet |
diet Flatfish gut histology liver Animal Science and Zoology |
description |
This article proposes a systematic evaluation of methods pertaining to the pathology discipline (histology, histochemistry, ultrastructure, in situ techniques, image analysis including morphom-etry and fractal dimension analysis) employed to study liver and intestine status during a series of nutritional trials performed on flatfish species (common sole, Solea solea; Senegal sole, Solea senegalensis and turbot, Scophthalmus maximus) of commercial interest. Histology is the first step able to detect the normal architecture of the tissues or the possible occurring changes related to inflammation, degeneration and cell death. The evaluation of liver should include a scoring system to obtain semi-quantitative numerical data that are statistically analysable; histochemisty is a valid tool for identifying the type of cell content, together with ultrastructure that also permits the detection of even mild signs of cell damage. For the evaluation of the intestine, in situ techniques are useful together with fractal dimension analysis to determine the mucosal tro-phism, while ultrastructure can demonstrate cell injury. These considerations were resumed in a methodological flowchart as a valid tool to assess the fish health status when nutritional trials are planned. |
author2 |
Sirri, Rubina Sarli, Giuseppe Bianco, Carlo Bonaldo, Alessio Gatta, Pier Paolo Fontanillas, Ramon De Vico, Gionata Carella, Francesca Brachelente, Chiara Parma, Luca Mandrioli, Luciana |
format |
Article in Journal/Newspaper |
author |
Sirri, Rubina Sarli, Giuseppe Bianco, Carlo Bonaldo, Alessio Gatta, Pier Paolo Fontanillas, Ramon De Vico, Gionata Carella, Francesca Brachelente, Chiara Parma, Luca Mandrioli, Luciana |
author_facet |
Sirri, Rubina Sarli, Giuseppe Bianco, Carlo Bonaldo, Alessio Gatta, Pier Paolo Fontanillas, Ramon De Vico, Gionata Carella, Francesca Brachelente, Chiara Parma, Luca Mandrioli, Luciana |
author_sort |
Sirri, Rubina |
title |
Retrospective study of pathology-based investigative techniques for the assessment of diet-induced changes in liver and intestine of flatfish |
title_short |
Retrospective study of pathology-based investigative techniques for the assessment of diet-induced changes in liver and intestine of flatfish |
title_full |
Retrospective study of pathology-based investigative techniques for the assessment of diet-induced changes in liver and intestine of flatfish |
title_fullStr |
Retrospective study of pathology-based investigative techniques for the assessment of diet-induced changes in liver and intestine of flatfish |
title_full_unstemmed |
Retrospective study of pathology-based investigative techniques for the assessment of diet-induced changes in liver and intestine of flatfish |
title_sort |
retrospective study of pathology-based investigative techniques for the assessment of diet-induced changes in liver and intestine of flatfish |
publishDate |
2018 |
url |
http://hdl.handle.net/11588/702005 https://doi.org/10.1080/1828051X.2017.1364610 http://www.tandfonline.com/loi/tjas20#.VpSlwLfUhFo |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000424424400031 volume:17 issue:2 firstpage:518 lastpage:529 numberofpages:12 journal:ITALIAN JOURNAL OF ANIMAL SCIENCE http://hdl.handle.net/11588/702005 doi:10.1080/1828051X.2017.1364610 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85041555293 http://www.tandfonline.com/loi/tjas20#.VpSlwLfUhFo |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1080/1828051X.2017.1364610 |
container_title |
Italian Journal of Animal Science |
container_volume |
17 |
container_issue |
2 |
container_start_page |
518 |
op_container_end_page |
529 |
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1802649869894025216 |