Ecology of cold environments: new insights of bacterial metabolic adaptation through an integrated genomic-phenomic approach
Cold environments dominate Earth's biosphere, hosting complex microbial communities with the ability to thrive at low temperatures. However, the underlying molecular mechanisms and the metabolic pathways involved in bacterial cold-adaptation mechanisms are still not fully understood. Herein, we...
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ftunivnapoliiris:oai:www.iris.unina.it:11588/671956 2024-06-23T07:47:38+00:00 Ecology of cold environments: new insights of bacterial metabolic adaptation through an integrated genomic-phenomic approach Mocali, Stefano Chiellini, Carolina Fabiani, Arturo Decuzzi, Silvia de Pascale, Donatella PARRILLI, ERMENEGILDA TUTINO, MARIA LUISA Perrin, Elena Bosi, Emanuele Fondi, Marco Lo Giudice, Angelina Fani, Renato Mocali, Stefano Chiellini, Carolina Fabiani, Arturo Decuzzi, Silvia de Pascale, Donatella Parrilli, Ermenegilda Tutino, MARIA LUISA Perrin, Elena Bosi, Emanuele Fondi, Marco Lo Giudice, Angelina Fani, Renato 2017 http://hdl.handle.net/11588/671956 https://doi.org/10.1038/s41598-017-00876-4 eng eng info:eu-repo/semantics/altIdentifier/wos/WOS:000399186000003 volume:7 issue:1-article number 839 firstpage:1 lastpage:13 numberofpages:13 journal:SCIENTIFIC REPORTS http://hdl.handle.net/11588/671956 doi:10.1038/s41598-017-00876-4 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85018278882 info:eu-repo/semantics/article 2017 ftunivnapoliiris https://doi.org/10.1038/s41598-017-00876-4 2024-06-10T14:58:50Z Cold environments dominate Earth's biosphere, hosting complex microbial communities with the ability to thrive at low temperatures. However, the underlying molecular mechanisms and the metabolic pathways involved in bacterial cold-adaptation mechanisms are still not fully understood. Herein, we assessed the metabolic features of the Antarctic bacterium Pseudoalteromonas haloplanktis TAC125 (PhTAC125), a model organism for cold-adaptation, at both 4 °C and 15 °C, by integrating genomic and phenomic (high-throughput phenotyping) data and comparing the obtained results to the taxonomically related Antarctic bacterium Pseudoalteromonas sp. TB41 (PspTB41). Although the genome size of PspTB41 is considerably larger than PhTAC125, the higher number of genes did not reflect any higher metabolic versatility at 4 °C as compared to PhTAC125. Remarkably, protein S-thiolation regulated by glutathione and glutathionylspermidine appeared to be a new possible mechanism for cold adaptation in PhTAC125. More in general, this study represents an example of how 'multi-omic' information might potentially contribute in filling the gap between genotypic and phenotypic features related to cold-adaptation mechanisms in bacteria. Article in Journal/Newspaper Antarc* Antarctic IRIS Università degli Studi di Napoli Federico II Antarctic The Antarctic Scientific Reports 7 1 |
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Open Polar |
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IRIS Università degli Studi di Napoli Federico II |
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ftunivnapoliiris |
language |
English |
description |
Cold environments dominate Earth's biosphere, hosting complex microbial communities with the ability to thrive at low temperatures. However, the underlying molecular mechanisms and the metabolic pathways involved in bacterial cold-adaptation mechanisms are still not fully understood. Herein, we assessed the metabolic features of the Antarctic bacterium Pseudoalteromonas haloplanktis TAC125 (PhTAC125), a model organism for cold-adaptation, at both 4 °C and 15 °C, by integrating genomic and phenomic (high-throughput phenotyping) data and comparing the obtained results to the taxonomically related Antarctic bacterium Pseudoalteromonas sp. TB41 (PspTB41). Although the genome size of PspTB41 is considerably larger than PhTAC125, the higher number of genes did not reflect any higher metabolic versatility at 4 °C as compared to PhTAC125. Remarkably, protein S-thiolation regulated by glutathione and glutathionylspermidine appeared to be a new possible mechanism for cold adaptation in PhTAC125. More in general, this study represents an example of how 'multi-omic' information might potentially contribute in filling the gap between genotypic and phenotypic features related to cold-adaptation mechanisms in bacteria. |
author2 |
Mocali, Stefano Chiellini, Carolina Fabiani, Arturo Decuzzi, Silvia de Pascale, Donatella Parrilli, Ermenegilda Tutino, MARIA LUISA Perrin, Elena Bosi, Emanuele Fondi, Marco Lo Giudice, Angelina Fani, Renato |
format |
Article in Journal/Newspaper |
author |
Mocali, Stefano Chiellini, Carolina Fabiani, Arturo Decuzzi, Silvia de Pascale, Donatella PARRILLI, ERMENEGILDA TUTINO, MARIA LUISA Perrin, Elena Bosi, Emanuele Fondi, Marco Lo Giudice, Angelina Fani, Renato |
spellingShingle |
Mocali, Stefano Chiellini, Carolina Fabiani, Arturo Decuzzi, Silvia de Pascale, Donatella PARRILLI, ERMENEGILDA TUTINO, MARIA LUISA Perrin, Elena Bosi, Emanuele Fondi, Marco Lo Giudice, Angelina Fani, Renato Ecology of cold environments: new insights of bacterial metabolic adaptation through an integrated genomic-phenomic approach |
author_facet |
Mocali, Stefano Chiellini, Carolina Fabiani, Arturo Decuzzi, Silvia de Pascale, Donatella PARRILLI, ERMENEGILDA TUTINO, MARIA LUISA Perrin, Elena Bosi, Emanuele Fondi, Marco Lo Giudice, Angelina Fani, Renato |
author_sort |
Mocali, Stefano |
title |
Ecology of cold environments: new insights of bacterial metabolic adaptation through an integrated genomic-phenomic approach |
title_short |
Ecology of cold environments: new insights of bacterial metabolic adaptation through an integrated genomic-phenomic approach |
title_full |
Ecology of cold environments: new insights of bacterial metabolic adaptation through an integrated genomic-phenomic approach |
title_fullStr |
Ecology of cold environments: new insights of bacterial metabolic adaptation through an integrated genomic-phenomic approach |
title_full_unstemmed |
Ecology of cold environments: new insights of bacterial metabolic adaptation through an integrated genomic-phenomic approach |
title_sort |
ecology of cold environments: new insights of bacterial metabolic adaptation through an integrated genomic-phenomic approach |
publishDate |
2017 |
url |
http://hdl.handle.net/11588/671956 https://doi.org/10.1038/s41598-017-00876-4 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000399186000003 volume:7 issue:1-article number 839 firstpage:1 lastpage:13 numberofpages:13 journal:SCIENTIFIC REPORTS http://hdl.handle.net/11588/671956 doi:10.1038/s41598-017-00876-4 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85018278882 |
op_doi |
https://doi.org/10.1038/s41598-017-00876-4 |
container_title |
Scientific Reports |
container_volume |
7 |
container_issue |
1 |
_version_ |
1802651768237064192 |