Stabilization of Candida antarctica Lipase B (CALB) immobilized on octyl agarose by treatment with polyethyleneimine (PEI)

• Published in: Molecules
• Main Authors: PEIRCE, SARA, MARZOCCHELLA, ANTONIO, Tacias Pascacio, Veymar G., Russo, Maria Elena, Virgen Ortíz, José J, Fernandez Lafuente, Roberto
• Other Authors: Peirce, Sara, Marzocchella, Antonio
• Format: Article in Journal/Newspaper
• Language: English
• Published: 2016
• Subjects: Enzyme physical intermolecular crosslinking; Enzyme stabilization; Interfacial adsorption; PEI modification; Reversible immobilization; Organic Chemistry; Triton; Antarc*; Antarctica
• Online Access: http://hdl.handle.net/11588/637542; https://doi.org/10.3390/molecules21060751; http://www.mdpi.com/1420-3049/21/6/751/pdf

Lipase B from Candida antarctica (CALB) was immobilized on octyl agarose (OC) and physically modified with polyethyleneimine (PEI) in order to confer a strong ion exchange character to the enzyme and thus enable the immobilization of other enzymes on its surface. The enzyme activity was fully mainta...