Soluble recombinant protein production in Pseudoalteromonas haloplanktis TAC125
Solubility/activity issues are often experienced when immunoglobulin fragments are produced in conventional microbial cell factories. Although several experimental approaches have been followed to solve, or at least minimize, the accumulation of the recombinant proteins into insoluble aggregates, so...
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ftunivnapoliiris:oai:www.iris.unina.it:11588/612495 2024-09-09T19:10:21+00:00 Soluble recombinant protein production in Pseudoalteromonas haloplanktis TAC125 Giuliani, Maria PARRILLI, ERMENEGILDA Sannino, Filomena Apuzzo, Gennaro MARINO, GENNARO TUTINO, MARIA LUISA Elena Garcìa-Fruitòs Giuliani, Maria Parrilli, Ermenegilda Sannino, Filomena Apuzzo, Gennaro Marino, Gennaro Tutino, MARIA LUISA 2015 http://hdl.handle.net/11588/612495 https://doi.org/10.1007/978-1-4939-2205-5_13 eng eng Humana Press info:eu-repo/semantics/altIdentifier/isbn/978-1-4939-2204-8 info:eu-repo/semantics/altIdentifier/isbn/978-1-4939-2205-5 info:eu-repo/semantics/altIdentifier/wos/WOS:000350415600014 ispartofbook:Insoluble proteins volume:1258 firstpage:243 lastpage:257 numberofpages:15 serie:METHODS IN MOLECULAR BIOLOGY alleditors:Elena Garcìa-Fruitòs http://hdl.handle.net/11588/612495 doi:10.1007/978-1-4939-2205-5_13 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84958643947 Batch fermentation LIV medium Pseudoalteromonas haloplanktis TAC125 Psychrophilic gene expression system VHHD6.1 info:eu-repo/semantics/bookPart 2015 ftunivnapoliiris https://doi.org/10.1007/978-1-4939-2205-5_13 2024-06-17T15:19:31Z Solubility/activity issues are often experienced when immunoglobulin fragments are produced in conventional microbial cell factories. Although several experimental approaches have been followed to solve, or at least minimize, the accumulation of the recombinant proteins into insoluble aggregates, sometimes the only alternative strategy is changing the protein production platform. In this chapter we describe the use of Antarctic bacterium Pseudoalteromonas haloplanktis TAC125 as host of choice for the production of the heavy-chain antibody fragment VHHD6.1. Combining the use of a regulated psychrophilic gene expression system with an optimized fermentation process in defined growth medium, we obtained the recombinant VHHD6.1 in fully soluble form and correctly translocated into host periplasmic space. Book Part Antarc* Antarctic IRIS Università degli Studi di Napoli Federico II Antarctic 243 257 |
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Open Polar |
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IRIS Università degli Studi di Napoli Federico II |
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ftunivnapoliiris |
language |
English |
topic |
Batch fermentation LIV medium Pseudoalteromonas haloplanktis TAC125 Psychrophilic gene expression system VHHD6.1 |
spellingShingle |
Batch fermentation LIV medium Pseudoalteromonas haloplanktis TAC125 Psychrophilic gene expression system VHHD6.1 Giuliani, Maria PARRILLI, ERMENEGILDA Sannino, Filomena Apuzzo, Gennaro MARINO, GENNARO TUTINO, MARIA LUISA Soluble recombinant protein production in Pseudoalteromonas haloplanktis TAC125 |
topic_facet |
Batch fermentation LIV medium Pseudoalteromonas haloplanktis TAC125 Psychrophilic gene expression system VHHD6.1 |
description |
Solubility/activity issues are often experienced when immunoglobulin fragments are produced in conventional microbial cell factories. Although several experimental approaches have been followed to solve, or at least minimize, the accumulation of the recombinant proteins into insoluble aggregates, sometimes the only alternative strategy is changing the protein production platform. In this chapter we describe the use of Antarctic bacterium Pseudoalteromonas haloplanktis TAC125 as host of choice for the production of the heavy-chain antibody fragment VHHD6.1. Combining the use of a regulated psychrophilic gene expression system with an optimized fermentation process in defined growth medium, we obtained the recombinant VHHD6.1 in fully soluble form and correctly translocated into host periplasmic space. |
author2 |
Elena Garcìa-Fruitòs Giuliani, Maria Parrilli, Ermenegilda Sannino, Filomena Apuzzo, Gennaro Marino, Gennaro Tutino, MARIA LUISA |
format |
Book Part |
author |
Giuliani, Maria PARRILLI, ERMENEGILDA Sannino, Filomena Apuzzo, Gennaro MARINO, GENNARO TUTINO, MARIA LUISA |
author_facet |
Giuliani, Maria PARRILLI, ERMENEGILDA Sannino, Filomena Apuzzo, Gennaro MARINO, GENNARO TUTINO, MARIA LUISA |
author_sort |
Giuliani, Maria |
title |
Soluble recombinant protein production in Pseudoalteromonas haloplanktis TAC125 |
title_short |
Soluble recombinant protein production in Pseudoalteromonas haloplanktis TAC125 |
title_full |
Soluble recombinant protein production in Pseudoalteromonas haloplanktis TAC125 |
title_fullStr |
Soluble recombinant protein production in Pseudoalteromonas haloplanktis TAC125 |
title_full_unstemmed |
Soluble recombinant protein production in Pseudoalteromonas haloplanktis TAC125 |
title_sort |
soluble recombinant protein production in pseudoalteromonas haloplanktis tac125 |
publisher |
Humana Press |
publishDate |
2015 |
url |
http://hdl.handle.net/11588/612495 https://doi.org/10.1007/978-1-4939-2205-5_13 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
info:eu-repo/semantics/altIdentifier/isbn/978-1-4939-2204-8 info:eu-repo/semantics/altIdentifier/isbn/978-1-4939-2205-5 info:eu-repo/semantics/altIdentifier/wos/WOS:000350415600014 ispartofbook:Insoluble proteins volume:1258 firstpage:243 lastpage:257 numberofpages:15 serie:METHODS IN MOLECULAR BIOLOGY alleditors:Elena Garcìa-Fruitòs http://hdl.handle.net/11588/612495 doi:10.1007/978-1-4939-2205-5_13 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84958643947 |
op_doi |
https://doi.org/10.1007/978-1-4939-2205-5_13 |
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243 |
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257 |
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1809825241279496192 |