A unique capsular polysaccharide structure from the psy-chrophilic marine bacterium Colwellia psychrerythraea 34H that mimicks antifreeze (glyco) proteins
The low temperatures of polar regions and high-altitude environments, especially icy habitats, present challenges for many microorganisms. Their ability to live under subfreezing conditions implies the production of compounds conferring cryotolerance. Colwellia psychrerythraea 34H, a γ-proteobacteri...
Published in: | Journal of the American Chemical Society |
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Online Access: | http://hdl.handle.net/11588/598838 https://doi.org/10.1021/ja5075954 |
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ftunivnapoliiris:oai:www.iris.unina.it:11588/598838 2024-09-09T19:25:27+00:00 A unique capsular polysaccharide structure from the psy-chrophilic marine bacterium Colwellia psychrerythraea 34H that mimicks antifreeze (glyco) proteins CARILLO, SARA CASILLO, ANGELA PIERETTI, GIUSEPPINA PARRILLI, ERMENEGILDA SANNINO, FILOMENA Maddalena Bayer Giraldi Sandro Cosconati NOVELLINO, ETTORE Marcela Ewert Jody W. Deming LANZETTA, ROSA MARINO, GENNARO PARRILLI, MICHELANGELO RANDAZZO, ANTONIO TUTINO, MARIA LUISA CORSARO, MARIA MICHELA Carillo, Sara Casillo, Angela Pieretti, Giuseppina Parrilli, Ermenegilda Sannino, Filomena Maddalena Bayer, Giraldi Sandro, Cosconati Novellino, Ettore Marcela, Ewert Jody W., Deming Lanzetta, Rosa Marino, Gennaro Parrilli, Michelangelo Randazzo, Antonio Tutino, MARIA LUISA Corsaro, MARIA MICHELA 2015 http://hdl.handle.net/11588/598838 https://doi.org/10.1021/ja5075954 eng eng info:eu-repo/semantics/altIdentifier/wos/WOS:000348483500044 volume:137 issue:1 firstpage:179 lastpage:189 numberofpages:11 journal:JOURNAL OF THE AMERICAN CHEMICAL SOCIETY http://hdl.handle.net/11588/598838 doi:10.1021/ja5075954 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84921033477 info:eu-repo/semantics/restrictedAccess info:eu-repo/semantics/article 2015 ftunivnapoliiris https://doi.org/10.1021/ja5075954 2024-06-17T15:19:22Z The low temperatures of polar regions and high-altitude environments, especially icy habitats, present challenges for many microorganisms. Their ability to live under subfreezing conditions implies the production of compounds conferring cryotolerance. Colwellia psychrerythraea 34H, a γ-proteobacterium isolated from subzero Arctic marine sediments, provides a model for the study of life in cold environments. We report here the identification and detailed molecular primary and secondary structures of capsular polysaccharide from C. psychrerythraea 34H cells. The polymer was isolated in the water layer when cells were extracted by phenol/water and characterized by one- and two-dimensional NMR spectroscopy together with chemical analysis. Molecular mechanics and dynamics calculations were also performed. The polysaccharide consists of a tetrasaccharidic repeating unit containing two amino sugars and two uronic acids bearing threonine as substituent. The structural features of this unique polysaccharide resemble those present in antifreeze proteins and glycoproteins. These results suggest a possible correlation between the capsule structure and the ability of C. psychrerythraea to colonize subfreezing marine environments. Article in Journal/Newspaper Arctic IRIS Università degli Studi di Napoli Federico II Arctic Journal of the American Chemical Society 137 1 179 189 |
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Open Polar |
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IRIS Università degli Studi di Napoli Federico II |
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ftunivnapoliiris |
language |
English |
description |
The low temperatures of polar regions and high-altitude environments, especially icy habitats, present challenges for many microorganisms. Their ability to live under subfreezing conditions implies the production of compounds conferring cryotolerance. Colwellia psychrerythraea 34H, a γ-proteobacterium isolated from subzero Arctic marine sediments, provides a model for the study of life in cold environments. We report here the identification and detailed molecular primary and secondary structures of capsular polysaccharide from C. psychrerythraea 34H cells. The polymer was isolated in the water layer when cells were extracted by phenol/water and characterized by one- and two-dimensional NMR spectroscopy together with chemical analysis. Molecular mechanics and dynamics calculations were also performed. The polysaccharide consists of a tetrasaccharidic repeating unit containing two amino sugars and two uronic acids bearing threonine as substituent. The structural features of this unique polysaccharide resemble those present in antifreeze proteins and glycoproteins. These results suggest a possible correlation between the capsule structure and the ability of C. psychrerythraea to colonize subfreezing marine environments. |
author2 |
Carillo, Sara Casillo, Angela Pieretti, Giuseppina Parrilli, Ermenegilda Sannino, Filomena Maddalena Bayer, Giraldi Sandro, Cosconati Novellino, Ettore Marcela, Ewert Jody W., Deming Lanzetta, Rosa Marino, Gennaro Parrilli, Michelangelo Randazzo, Antonio Tutino, MARIA LUISA Corsaro, MARIA MICHELA |
format |
Article in Journal/Newspaper |
author |
CARILLO, SARA CASILLO, ANGELA PIERETTI, GIUSEPPINA PARRILLI, ERMENEGILDA SANNINO, FILOMENA Maddalena Bayer Giraldi Sandro Cosconati NOVELLINO, ETTORE Marcela Ewert Jody W. Deming LANZETTA, ROSA MARINO, GENNARO PARRILLI, MICHELANGELO RANDAZZO, ANTONIO TUTINO, MARIA LUISA CORSARO, MARIA MICHELA |
spellingShingle |
CARILLO, SARA CASILLO, ANGELA PIERETTI, GIUSEPPINA PARRILLI, ERMENEGILDA SANNINO, FILOMENA Maddalena Bayer Giraldi Sandro Cosconati NOVELLINO, ETTORE Marcela Ewert Jody W. Deming LANZETTA, ROSA MARINO, GENNARO PARRILLI, MICHELANGELO RANDAZZO, ANTONIO TUTINO, MARIA LUISA CORSARO, MARIA MICHELA A unique capsular polysaccharide structure from the psy-chrophilic marine bacterium Colwellia psychrerythraea 34H that mimicks antifreeze (glyco) proteins |
author_facet |
CARILLO, SARA CASILLO, ANGELA PIERETTI, GIUSEPPINA PARRILLI, ERMENEGILDA SANNINO, FILOMENA Maddalena Bayer Giraldi Sandro Cosconati NOVELLINO, ETTORE Marcela Ewert Jody W. Deming LANZETTA, ROSA MARINO, GENNARO PARRILLI, MICHELANGELO RANDAZZO, ANTONIO TUTINO, MARIA LUISA CORSARO, MARIA MICHELA |
author_sort |
CARILLO, SARA |
title |
A unique capsular polysaccharide structure from the psy-chrophilic marine bacterium Colwellia psychrerythraea 34H that mimicks antifreeze (glyco) proteins |
title_short |
A unique capsular polysaccharide structure from the psy-chrophilic marine bacterium Colwellia psychrerythraea 34H that mimicks antifreeze (glyco) proteins |
title_full |
A unique capsular polysaccharide structure from the psy-chrophilic marine bacterium Colwellia psychrerythraea 34H that mimicks antifreeze (glyco) proteins |
title_fullStr |
A unique capsular polysaccharide structure from the psy-chrophilic marine bacterium Colwellia psychrerythraea 34H that mimicks antifreeze (glyco) proteins |
title_full_unstemmed |
A unique capsular polysaccharide structure from the psy-chrophilic marine bacterium Colwellia psychrerythraea 34H that mimicks antifreeze (glyco) proteins |
title_sort |
unique capsular polysaccharide structure from the psy-chrophilic marine bacterium colwellia psychrerythraea 34h that mimicks antifreeze (glyco) proteins |
publishDate |
2015 |
url |
http://hdl.handle.net/11588/598838 https://doi.org/10.1021/ja5075954 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000348483500044 volume:137 issue:1 firstpage:179 lastpage:189 numberofpages:11 journal:JOURNAL OF THE AMERICAN CHEMICAL SOCIETY http://hdl.handle.net/11588/598838 doi:10.1021/ja5075954 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84921033477 |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1021/ja5075954 |
container_title |
Journal of the American Chemical Society |
container_volume |
137 |
container_issue |
1 |
container_start_page |
179 |
op_container_end_page |
189 |
_version_ |
1809895245435895808 |