A rolling-circle plasmid from Psychrobacter sp. TA144: Evidence for a novel Rep subfamily
In this paper we report the cloning and sequencing of two small plasmids, pTAUp and pTADw, from the Antarctic Gram-negative Psychrobacter sp strain TA144. The observation that pTAUp contains a putative Rep-coding gene (Psyrep) suggested that its duplication occurs via a rolling-circle replication me...
Published in: | Biochemical and Biophysical Research Communications |
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Online Access: | http://hdl.handle.net/11588/480514 https://doi.org/10.1006/bbrc.2000.3148 |
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ftunivnapoliiris:oai:www.iris.unina.it:11588/480514 2024-06-23T07:47:22+00:00 A rolling-circle plasmid from Psychrobacter sp. TA144: Evidence for a novel Rep subfamily TUTINO, MARIA LUISA DUILIO, ANGELA SANNIA, GIOVANNI M. A. Moretti G. Marino Tutino, MARIA LUISA Duilio, Angela M. A., Moretti Sannia, Giovanni G., Marino 2000 STAMPA http://hdl.handle.net/11588/480514 https://doi.org/10.1006/bbrc.2000.3148 eng eng info:eu-repo/semantics/altIdentifier/pmid/10913365 info:eu-repo/semantics/altIdentifier/wos/WOS:000088592500036 volume:274 issue:2 firstpage:488 lastpage:495 numberofpages:8 journal:BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS http://hdl.handle.net/11588/480514 doi:10.1006/bbrc.2000.3148 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-0034596254 DNA-REPLICATION SEQUENCE MUTAGENESIS REPLICONS Antarctic bacterium Psychrobacter sp. TA144 info:eu-repo/semantics/article 2000 ftunivnapoliiris https://doi.org/10.1006/bbrc.2000.3148 2024-06-10T14:58:48Z In this paper we report the cloning and sequencing of two small plasmids, pTAUp and pTADw, from the Antarctic Gram-negative Psychrobacter sp strain TA144. The observation that pTAUp contains a putative Rep-coding gene (Psyrep) suggested that its duplication occurs via a rolling-circle replication mechanism. This hypothesis was confirmed by the identification of the pTAUp single-stranded DNA form. The putative pTAUp plus origin of replication was found at the 3' end of the Psyrep by using an in vivo complementation assay. Structural similarities at the level of (i) gene organization, (ii) protein sequence, and (iii) nick site sequences strongly suggest that the psychrophilic enzyme belongs to a new subfamily of replication enzymes, (C) 2000 Academic Press. Article in Journal/Newspaper Antarc* Antarctic IRIS Università degli Studi di Napoli Federico II Antarctic The Antarctic Biochemical and Biophysical Research Communications 274 2 488 495 |
institution |
Open Polar |
collection |
IRIS Università degli Studi di Napoli Federico II |
op_collection_id |
ftunivnapoliiris |
language |
English |
topic |
DNA-REPLICATION SEQUENCE MUTAGENESIS REPLICONS Antarctic bacterium Psychrobacter sp. TA144 |
spellingShingle |
DNA-REPLICATION SEQUENCE MUTAGENESIS REPLICONS Antarctic bacterium Psychrobacter sp. TA144 TUTINO, MARIA LUISA DUILIO, ANGELA SANNIA, GIOVANNI M. A. Moretti G. Marino A rolling-circle plasmid from Psychrobacter sp. TA144: Evidence for a novel Rep subfamily |
topic_facet |
DNA-REPLICATION SEQUENCE MUTAGENESIS REPLICONS Antarctic bacterium Psychrobacter sp. TA144 |
description |
In this paper we report the cloning and sequencing of two small plasmids, pTAUp and pTADw, from the Antarctic Gram-negative Psychrobacter sp strain TA144. The observation that pTAUp contains a putative Rep-coding gene (Psyrep) suggested that its duplication occurs via a rolling-circle replication mechanism. This hypothesis was confirmed by the identification of the pTAUp single-stranded DNA form. The putative pTAUp plus origin of replication was found at the 3' end of the Psyrep by using an in vivo complementation assay. Structural similarities at the level of (i) gene organization, (ii) protein sequence, and (iii) nick site sequences strongly suggest that the psychrophilic enzyme belongs to a new subfamily of replication enzymes, (C) 2000 Academic Press. |
author2 |
Tutino, MARIA LUISA Duilio, Angela M. A., Moretti Sannia, Giovanni G., Marino |
format |
Article in Journal/Newspaper |
author |
TUTINO, MARIA LUISA DUILIO, ANGELA SANNIA, GIOVANNI M. A. Moretti G. Marino |
author_facet |
TUTINO, MARIA LUISA DUILIO, ANGELA SANNIA, GIOVANNI M. A. Moretti G. Marino |
author_sort |
TUTINO, MARIA LUISA |
title |
A rolling-circle plasmid from Psychrobacter sp. TA144: Evidence for a novel Rep subfamily |
title_short |
A rolling-circle plasmid from Psychrobacter sp. TA144: Evidence for a novel Rep subfamily |
title_full |
A rolling-circle plasmid from Psychrobacter sp. TA144: Evidence for a novel Rep subfamily |
title_fullStr |
A rolling-circle plasmid from Psychrobacter sp. TA144: Evidence for a novel Rep subfamily |
title_full_unstemmed |
A rolling-circle plasmid from Psychrobacter sp. TA144: Evidence for a novel Rep subfamily |
title_sort |
rolling-circle plasmid from psychrobacter sp. ta144: evidence for a novel rep subfamily |
publishDate |
2000 |
url |
http://hdl.handle.net/11588/480514 https://doi.org/10.1006/bbrc.2000.3148 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/10913365 info:eu-repo/semantics/altIdentifier/wos/WOS:000088592500036 volume:274 issue:2 firstpage:488 lastpage:495 numberofpages:8 journal:BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS http://hdl.handle.net/11588/480514 doi:10.1006/bbrc.2000.3148 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-0034596254 |
op_doi |
https://doi.org/10.1006/bbrc.2000.3148 |
container_title |
Biochemical and Biophysical Research Communications |
container_volume |
274 |
container_issue |
2 |
container_start_page |
488 |
op_container_end_page |
495 |
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1802651453487054848 |