Karyology of the Antarctic scallop Adamussium colbecki, with some comments on the karyological evolution of pectinids
Karyotype, location of the nucleolar organiser region (NOR) and heterochromatin presence and composition were studied in the Antarctic scallop Adamussium colbecki Smith, 1902. The karyotype exhibits 2n = 38 chromosomes with 11 pairs of metacentrics, 5 of submetacentrics, one subtelocentric and two t...
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Online Access: | http://hdl.handle.net/11588/464724 https://doi.org/10.1007/s10709-005-5366-8 |
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ftunivnapoliiris:oai:www.iris.unina.it:11588/464724 2023-05-15T14:04:34+02:00 Karyology of the Antarctic scallop Adamussium colbecki, with some comments on the karyological evolution of pectinids ODIERNA, GAETANO APREA, GENNARO CAPRIGLIONE, TERESA M. Barucca A. Canapa E. Olmo Odierna, Gaetano Aprea, Gennaro M., Barucca A., Canapa Capriglione, Teresa E., Olmo 2006 http://hdl.handle.net/11588/464724 https://doi.org/10.1007/s10709-005-5366-8 unknown info:eu-repo/semantics/altIdentifier/wos/WOS:000239164900031 volume:127 firstpage:341 lastpage:349 numberofpages:8 journal:GENETICA http://hdl.handle.net/11588/464724 doi:10.1007/s10709-005-5366-8 info:eu-repo/semantics/article 2006 ftunivnapoliiris https://doi.org/10.1007/s10709-005-5366-8 2023-03-14T23:35:20Z Karyotype, location of the nucleolar organiser region (NOR) and heterochromatin presence and composition were studied in the Antarctic scallop Adamussium colbecki Smith, 1902. The karyotype exhibits 2n = 38 chromosomes with 11 pairs of metacentrics, 5 of submetacentrics, one subtelocentric and two telocentrics. Ag-NOR, CMA(3), DA/MM and NOR-FISH evidenced paracentromeric NORs on the short arm of 2nd pair chromosomes. Digestion with three restriction endonucleases followed by sequential staining with Giemsa, CMA(3) and DAPI evidenced on all chromosomes centromeric heterochromatin positive for both DAPI and CMA(3). In situ hybridisation analysis showed the presence of an AT-rich satellite DNA in the centromeric heterochromatin of several chromosomes. A mosaicism was detected in the germinal cell lines of one specimen, as in six of the 20 plates examined the set had 37 chromosomes with a missing pair of telocentrics and an unpaired metacentric. Comparison of the chromosome sets of all the pectinids studied to date and comparison with a phyletic tree obtained from molecular mitochondrial genes studies yielded good agreement between karyotype morphology and taxonomic classification. Article in Journal/Newspaper Antarc* Antarctic IRIS Università degli Studi di Napoli Federico II Antarctic The Antarctic Genetica 127 1-3 341 349 |
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IRIS Università degli Studi di Napoli Federico II |
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ftunivnapoliiris |
language |
unknown |
description |
Karyotype, location of the nucleolar organiser region (NOR) and heterochromatin presence and composition were studied in the Antarctic scallop Adamussium colbecki Smith, 1902. The karyotype exhibits 2n = 38 chromosomes with 11 pairs of metacentrics, 5 of submetacentrics, one subtelocentric and two telocentrics. Ag-NOR, CMA(3), DA/MM and NOR-FISH evidenced paracentromeric NORs on the short arm of 2nd pair chromosomes. Digestion with three restriction endonucleases followed by sequential staining with Giemsa, CMA(3) and DAPI evidenced on all chromosomes centromeric heterochromatin positive for both DAPI and CMA(3). In situ hybridisation analysis showed the presence of an AT-rich satellite DNA in the centromeric heterochromatin of several chromosomes. A mosaicism was detected in the germinal cell lines of one specimen, as in six of the 20 plates examined the set had 37 chromosomes with a missing pair of telocentrics and an unpaired metacentric. Comparison of the chromosome sets of all the pectinids studied to date and comparison with a phyletic tree obtained from molecular mitochondrial genes studies yielded good agreement between karyotype morphology and taxonomic classification. |
author2 |
Odierna, Gaetano Aprea, Gennaro M., Barucca A., Canapa Capriglione, Teresa E., Olmo |
format |
Article in Journal/Newspaper |
author |
ODIERNA, GAETANO APREA, GENNARO CAPRIGLIONE, TERESA M. Barucca A. Canapa E. Olmo |
spellingShingle |
ODIERNA, GAETANO APREA, GENNARO CAPRIGLIONE, TERESA M. Barucca A. Canapa E. Olmo Karyology of the Antarctic scallop Adamussium colbecki, with some comments on the karyological evolution of pectinids |
author_facet |
ODIERNA, GAETANO APREA, GENNARO CAPRIGLIONE, TERESA M. Barucca A. Canapa E. Olmo |
author_sort |
ODIERNA, GAETANO |
title |
Karyology of the Antarctic scallop Adamussium colbecki, with some comments on the karyological evolution of pectinids |
title_short |
Karyology of the Antarctic scallop Adamussium colbecki, with some comments on the karyological evolution of pectinids |
title_full |
Karyology of the Antarctic scallop Adamussium colbecki, with some comments on the karyological evolution of pectinids |
title_fullStr |
Karyology of the Antarctic scallop Adamussium colbecki, with some comments on the karyological evolution of pectinids |
title_full_unstemmed |
Karyology of the Antarctic scallop Adamussium colbecki, with some comments on the karyological evolution of pectinids |
title_sort |
karyology of the antarctic scallop adamussium colbecki, with some comments on the karyological evolution of pectinids |
publishDate |
2006 |
url |
http://hdl.handle.net/11588/464724 https://doi.org/10.1007/s10709-005-5366-8 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000239164900031 volume:127 firstpage:341 lastpage:349 numberofpages:8 journal:GENETICA http://hdl.handle.net/11588/464724 doi:10.1007/s10709-005-5366-8 |
op_doi |
https://doi.org/10.1007/s10709-005-5366-8 |
container_title |
Genetica |
container_volume |
127 |
container_issue |
1-3 |
container_start_page |
341 |
op_container_end_page |
349 |
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1766275752485978112 |