Novel Strategy for the Construction of Genomic Mutants of the Antarctic Bacterium Pseudoalteromonas haloplanktis TAC125.
The sequencing and the annotation of the marine Antarctic Pseudoalteromonas haloplanktis TAC125 genome has paved the way to investigate on the molecular mechanisms involved in adaptation to cold conditions. The growing interest in this unique bacterium prompted the developing of several genetic tool...
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Argelia Lorence
2012
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ftunivnapoliiris:oai:www.iris.unina.it:11588/430327 2024-06-23T07:46:51+00:00 Novel Strategy for the Construction of Genomic Mutants of the Antarctic Bacterium Pseudoalteromonas haloplanktis TAC125. GIULIANI, MARIA PARRILLI, ERMENEGILDA PEZZELLA, Cinzia RIPPA, VALENTINA DUILIO, ANGELA MARINO, GENNARO TUTINO, MARIA LUISA Argelia Lorence Giuliani, Maria Parrilli, Ermenegilda Pezzella, Cinzia Rippa, Valentina Duilio, Angela Marino, Gennaro Tutino, MARIA LUISA 2012 http://hdl.handle.net/11588/430327 https://doi.org/10.1007/978-1-61779-433-9_11 eng eng Argelia Lorence country:USA place:New York info:eu-repo/semantics/altIdentifier/isbn/9781617794322 info:eu-repo/semantics/altIdentifier/wos/WOS:000303413800011 ispartofbook:Methods in Molecular Biology volume:824 firstpage:219 lastpage:233 numberofpages:15 http://hdl.handle.net/11588/430327 doi:10.1007/978-1-61779-433-9_11 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84855948070 Pseudoalteromonas haloplanktis TAC125 - Allelic exchange - Counterselectable marker - Suicide vector info:eu-repo/semantics/bookPart 2012 ftunivnapoliiris https://doi.org/10.1007/978-1-61779-433-9_11 2024-06-10T14:58:48Z The sequencing and the annotation of the marine Antarctic Pseudoalteromonas haloplanktis TAC125 genome has paved the way to investigate on the molecular mechanisms involved in adaptation to cold conditions. The growing interest in this unique bacterium prompted the developing of several genetic tools for studying it at the molecular level. To allow a deeper understanding of the PhTAC125 physiology a genetic system for the reverse genetics in this bacterium was developed. In the present work, we describe a practical technique for allelic exchange and/or gene inactivation by in-frame deletion and the use of a counterselectable marker in P. haloplanktis. The construction of suitable non-replicating plasmid and methods used to carry out a two-step integration-segregation strategy in this bacterium are reported in detail.Furthermore two examples, in which the developed methodology was applied to find out gene function or to construct genetically engineered bacterial strains, were described. Book Part Antarc* Antarctic IRIS Università degli Studi di Napoli Federico II Antarctic The Antarctic 219 233 |
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IRIS Università degli Studi di Napoli Federico II |
op_collection_id |
ftunivnapoliiris |
language |
English |
topic |
Pseudoalteromonas haloplanktis TAC125 - Allelic exchange - Counterselectable marker - Suicide vector |
spellingShingle |
Pseudoalteromonas haloplanktis TAC125 - Allelic exchange - Counterselectable marker - Suicide vector GIULIANI, MARIA PARRILLI, ERMENEGILDA PEZZELLA, Cinzia RIPPA, VALENTINA DUILIO, ANGELA MARINO, GENNARO TUTINO, MARIA LUISA Novel Strategy for the Construction of Genomic Mutants of the Antarctic Bacterium Pseudoalteromonas haloplanktis TAC125. |
topic_facet |
Pseudoalteromonas haloplanktis TAC125 - Allelic exchange - Counterselectable marker - Suicide vector |
description |
The sequencing and the annotation of the marine Antarctic Pseudoalteromonas haloplanktis TAC125 genome has paved the way to investigate on the molecular mechanisms involved in adaptation to cold conditions. The growing interest in this unique bacterium prompted the developing of several genetic tools for studying it at the molecular level. To allow a deeper understanding of the PhTAC125 physiology a genetic system for the reverse genetics in this bacterium was developed. In the present work, we describe a practical technique for allelic exchange and/or gene inactivation by in-frame deletion and the use of a counterselectable marker in P. haloplanktis. The construction of suitable non-replicating plasmid and methods used to carry out a two-step integration-segregation strategy in this bacterium are reported in detail.Furthermore two examples, in which the developed methodology was applied to find out gene function or to construct genetically engineered bacterial strains, were described. |
author2 |
Argelia Lorence Giuliani, Maria Parrilli, Ermenegilda Pezzella, Cinzia Rippa, Valentina Duilio, Angela Marino, Gennaro Tutino, MARIA LUISA |
format |
Book Part |
author |
GIULIANI, MARIA PARRILLI, ERMENEGILDA PEZZELLA, Cinzia RIPPA, VALENTINA DUILIO, ANGELA MARINO, GENNARO TUTINO, MARIA LUISA |
author_facet |
GIULIANI, MARIA PARRILLI, ERMENEGILDA PEZZELLA, Cinzia RIPPA, VALENTINA DUILIO, ANGELA MARINO, GENNARO TUTINO, MARIA LUISA |
author_sort |
GIULIANI, MARIA |
title |
Novel Strategy for the Construction of Genomic Mutants of the Antarctic Bacterium Pseudoalteromonas haloplanktis TAC125. |
title_short |
Novel Strategy for the Construction of Genomic Mutants of the Antarctic Bacterium Pseudoalteromonas haloplanktis TAC125. |
title_full |
Novel Strategy for the Construction of Genomic Mutants of the Antarctic Bacterium Pseudoalteromonas haloplanktis TAC125. |
title_fullStr |
Novel Strategy for the Construction of Genomic Mutants of the Antarctic Bacterium Pseudoalteromonas haloplanktis TAC125. |
title_full_unstemmed |
Novel Strategy for the Construction of Genomic Mutants of the Antarctic Bacterium Pseudoalteromonas haloplanktis TAC125. |
title_sort |
novel strategy for the construction of genomic mutants of the antarctic bacterium pseudoalteromonas haloplanktis tac125. |
publisher |
Argelia Lorence |
publishDate |
2012 |
url |
http://hdl.handle.net/11588/430327 https://doi.org/10.1007/978-1-61779-433-9_11 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
info:eu-repo/semantics/altIdentifier/isbn/9781617794322 info:eu-repo/semantics/altIdentifier/wos/WOS:000303413800011 ispartofbook:Methods in Molecular Biology volume:824 firstpage:219 lastpage:233 numberofpages:15 http://hdl.handle.net/11588/430327 doi:10.1007/978-1-61779-433-9_11 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84855948070 |
op_doi |
https://doi.org/10.1007/978-1-61779-433-9_11 |
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219 |
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233 |
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1802648791782785024 |