Characterization of the volatile profile of Antarctic bacteria by using solid-phase microextraction-gas chromatography-mass spectrometry
Bacteria belonging to the Burkholderia cepacia complex (Bcc) are significant pathogens in Cystic Fibrosis (CF) patients and are resistant to a plethora of antibiotics. In this context, microorganisms from Antarctica are interesting because they produce antimicrobial compounds inhibiting the growth o...
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ftunivnapoliiris:oai:www.iris.unina.it:11588/430030 2024-09-09T19:04:27+00:00 Characterization of the volatile profile of Antarctic bacteria by using solid-phase microextraction-gas chromatography-mass spectrometry Romoli R. Papaleo M. C. de Pascale D. Michaud L. Logiudice A. Fani R. Bartolucci G. TUTINO, MARIA LUISA Romoli, R. Papaleo, M. C. de Pascale, D. Tutino, MARIA LUISA Michaud, L. Logiudice, A. Fani R. Bartolucci, G. 2011 http://hdl.handle.net/11588/430030 https://doi.org/10.1002/jms.1987 eng eng info:eu-repo/semantics/altIdentifier/wos/WOS:000297028200011 volume:46 issue:10 firstpage:1051 lastpage:1059 numberofpages:9 journal:JOURNAL OF MASS SPECTROMETRY http://hdl.handle.net/11588/430030 doi:10.1002/jms.1987 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-80054819141 Antarctic bacteria Volatile compound mass spectrometry info:eu-repo/semantics/article 2011 ftunivnapoliiris https://doi.org/10.1002/jms.1987 2024-06-17T15:19:27Z Bacteria belonging to the Burkholderia cepacia complex (Bcc) are significant pathogens in Cystic Fibrosis (CF) patients and are resistant to a plethora of antibiotics. In this context, microorganisms from Antarctica are interesting because they produce antimicrobial compounds inhibiting the growth of other bacteria. This is particularly true for bacteria isolated from Antarctic sponges. The aim of this work was to characterize a set of Antarctic bacteria for their ability to produce new natural drugs that could be exploited in the control of infections in CF patients by Bcc bacteria. Hence, 11 bacterial strains allocated to different genera (e.g., Pseudoalteromonas, Arthrobacter and Psychrobacter) were tested for their ability to inhibit the growth of 21 Bcc strains and some other human pathogens. All these bacteria completely inhibited the growth of most, if not all, Bcc strains, suggesting a highly specific activity toward Bcc strains. Experimental evidences showed that the antimicrobial compounds are small volatile organic compounds, and are constitutively produced via an unknown pathway. The microbial volatile profile was obtained by SPME-GC-MS within them/z interval of 40–450. Solid phasemicro extraction technique affords the possibility to extract the volatile compounds in head space with a minimal sample perturbation. Principal component analysis and successive cluster discriminant analysis was applied to evaluate the relationships among the volatile organic compounds with the aim of classifying the microorganisms by their volatile profile. These data highlight the potentiality of Antarctic bacteria as novel sources of antibacterial substances to face Bcc infections in CF patients. Article in Journal/Newspaper Antarc* Antarctic Antarctica IRIS Università degli Studi di Napoli Federico II Antarctic Journal of Mass Spectrometry 46 10 1051 1059 |
institution |
Open Polar |
collection |
IRIS Università degli Studi di Napoli Federico II |
op_collection_id |
ftunivnapoliiris |
language |
English |
topic |
Antarctic bacteria Volatile compound mass spectrometry |
spellingShingle |
Antarctic bacteria Volatile compound mass spectrometry Romoli R. Papaleo M. C. de Pascale D. Michaud L. Logiudice A. Fani R. Bartolucci G. TUTINO, MARIA LUISA Characterization of the volatile profile of Antarctic bacteria by using solid-phase microextraction-gas chromatography-mass spectrometry |
topic_facet |
Antarctic bacteria Volatile compound mass spectrometry |
description |
Bacteria belonging to the Burkholderia cepacia complex (Bcc) are significant pathogens in Cystic Fibrosis (CF) patients and are resistant to a plethora of antibiotics. In this context, microorganisms from Antarctica are interesting because they produce antimicrobial compounds inhibiting the growth of other bacteria. This is particularly true for bacteria isolated from Antarctic sponges. The aim of this work was to characterize a set of Antarctic bacteria for their ability to produce new natural drugs that could be exploited in the control of infections in CF patients by Bcc bacteria. Hence, 11 bacterial strains allocated to different genera (e.g., Pseudoalteromonas, Arthrobacter and Psychrobacter) were tested for their ability to inhibit the growth of 21 Bcc strains and some other human pathogens. All these bacteria completely inhibited the growth of most, if not all, Bcc strains, suggesting a highly specific activity toward Bcc strains. Experimental evidences showed that the antimicrobial compounds are small volatile organic compounds, and are constitutively produced via an unknown pathway. The microbial volatile profile was obtained by SPME-GC-MS within them/z interval of 40–450. Solid phasemicro extraction technique affords the possibility to extract the volatile compounds in head space with a minimal sample perturbation. Principal component analysis and successive cluster discriminant analysis was applied to evaluate the relationships among the volatile organic compounds with the aim of classifying the microorganisms by their volatile profile. These data highlight the potentiality of Antarctic bacteria as novel sources of antibacterial substances to face Bcc infections in CF patients. |
author2 |
Romoli, R. Papaleo, M. C. de Pascale, D. Tutino, MARIA LUISA Michaud, L. Logiudice, A. Fani R. Bartolucci, G. |
format |
Article in Journal/Newspaper |
author |
Romoli R. Papaleo M. C. de Pascale D. Michaud L. Logiudice A. Fani R. Bartolucci G. TUTINO, MARIA LUISA |
author_facet |
Romoli R. Papaleo M. C. de Pascale D. Michaud L. Logiudice A. Fani R. Bartolucci G. TUTINO, MARIA LUISA |
author_sort |
Romoli R. |
title |
Characterization of the volatile profile of Antarctic bacteria by using solid-phase microextraction-gas chromatography-mass spectrometry |
title_short |
Characterization of the volatile profile of Antarctic bacteria by using solid-phase microextraction-gas chromatography-mass spectrometry |
title_full |
Characterization of the volatile profile of Antarctic bacteria by using solid-phase microextraction-gas chromatography-mass spectrometry |
title_fullStr |
Characterization of the volatile profile of Antarctic bacteria by using solid-phase microextraction-gas chromatography-mass spectrometry |
title_full_unstemmed |
Characterization of the volatile profile of Antarctic bacteria by using solid-phase microextraction-gas chromatography-mass spectrometry |
title_sort |
characterization of the volatile profile of antarctic bacteria by using solid-phase microextraction-gas chromatography-mass spectrometry |
publishDate |
2011 |
url |
http://hdl.handle.net/11588/430030 https://doi.org/10.1002/jms.1987 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000297028200011 volume:46 issue:10 firstpage:1051 lastpage:1059 numberofpages:9 journal:JOURNAL OF MASS SPECTROMETRY http://hdl.handle.net/11588/430030 doi:10.1002/jms.1987 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-80054819141 |
op_doi |
https://doi.org/10.1002/jms.1987 |
container_title |
Journal of Mass Spectrometry |
container_volume |
46 |
container_issue |
10 |
container_start_page |
1051 |
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1059 |
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