Minimal Structural Requirements for Root Effect: The Crystal Structure of the Cathodic Hemoglobin Isolated from the Antarctic Fish Trematomus newnesi.
The cathodic hemoglobin component of the Antarctic fish Trematomus newnesi (HbCTn) is a Root-effect protein which displays peculiar features. The interpretation of its functional properties in relation to its sequence is puzzling. Indeed, HbCTn sequence is characterized by an extremely low histidyl...
Published in: | Proteins: Structure, Function, and Bioinformatics |
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ftunivnapoliiris:oai:www.iris.unina.it:11588/201965 2024-04-21T07:46:33+00:00 Minimal Structural Requirements for Root Effect: The Crystal Structure of the Cathodic Hemoglobin Isolated from the Antarctic Fish Trematomus newnesi. MAZZARELLA, LELIO MERLINO, ANTONELLO VERGARA, ALESSANDRO G. BONOMI M. LUBRANO A. RICCIO L. VITAGLIANO C. VERDE G. DI PRISCO Mazzarella, Lelio G., Bonomi M., Lubrano Merlino, Antonello A., Riccio Vergara, Alessandro L., Vitagliano C., Verde G., DI PRISCO 2006 STAMPA http://hdl.handle.net/11588/201965 https://doi.org/10.1002/prot.20709 eng eng info:eu-repo/semantics/altIdentifier/pmid/16299734 info:eu-repo/semantics/altIdentifier/wos/WOS:000234438800002 volume:62 issue:2 firstpage:316 lastpage:321 numberofpages:6 journal:PROTEINS http://hdl.handle.net/11588/201965 doi:10.1002/prot.20709 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-30144436769 info:eu-repo/semantics/closedAccess info:eu-repo/semantics/article 2006 ftunivnapoliiris https://doi.org/10.1002/prot.20709 2024-03-28T01:58:53Z The cathodic hemoglobin component of the Antarctic fish Trematomus newnesi (HbCTn) is a Root-effect protein which displays peculiar features. The interpretation of its functional properties in relation to its sequence is puzzling. Indeed, HbCTn sequence is characterized by an extremely low histidyl content, and in particular by the lack of His146b and His69b, which are believed to be important in Bohr and Root effects, respectively. Furthermore, previous analyses suggested that the local environment of Asp95α, Asp99β, and Asp 101β should not be appropriate for the formation of Asp-Asp interactions which are important for the Root effect. Here we report the high-resolution crystal structure of the deoxy form of HbCTn. Our data provide a structural interpretation for the very low oxygen affinity of the protein and insights into the structural determinants of the Root effect. The structure demonstrates that the presence of Ile41α and Ser97α at the a1b2 interface does not prevent the formation of the inter-Asp interactions in HbCTn, as previous studies had suggested. The present data indicate that the hydrogen bond formed between Asp95α and Asp101β, which is stabilized by Asp99β, is per se sufficient to generate the Root effect, and it is the minimal structural requirement needed for the design of Root-effect Hbs. Article in Journal/Newspaper Antarc* Antarctic IRIS Università degli Studi di Napoli Federico II Proteins: Structure, Function, and Bioinformatics 62 2 316 321 |
institution |
Open Polar |
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IRIS Università degli Studi di Napoli Federico II |
op_collection_id |
ftunivnapoliiris |
language |
English |
description |
The cathodic hemoglobin component of the Antarctic fish Trematomus newnesi (HbCTn) is a Root-effect protein which displays peculiar features. The interpretation of its functional properties in relation to its sequence is puzzling. Indeed, HbCTn sequence is characterized by an extremely low histidyl content, and in particular by the lack of His146b and His69b, which are believed to be important in Bohr and Root effects, respectively. Furthermore, previous analyses suggested that the local environment of Asp95α, Asp99β, and Asp 101β should not be appropriate for the formation of Asp-Asp interactions which are important for the Root effect. Here we report the high-resolution crystal structure of the deoxy form of HbCTn. Our data provide a structural interpretation for the very low oxygen affinity of the protein and insights into the structural determinants of the Root effect. The structure demonstrates that the presence of Ile41α and Ser97α at the a1b2 interface does not prevent the formation of the inter-Asp interactions in HbCTn, as previous studies had suggested. The present data indicate that the hydrogen bond formed between Asp95α and Asp101β, which is stabilized by Asp99β, is per se sufficient to generate the Root effect, and it is the minimal structural requirement needed for the design of Root-effect Hbs. |
author2 |
Mazzarella, Lelio G., Bonomi M., Lubrano Merlino, Antonello A., Riccio Vergara, Alessandro L., Vitagliano C., Verde G., DI PRISCO |
format |
Article in Journal/Newspaper |
author |
MAZZARELLA, LELIO MERLINO, ANTONELLO VERGARA, ALESSANDRO G. BONOMI M. LUBRANO A. RICCIO L. VITAGLIANO C. VERDE G. DI PRISCO |
spellingShingle |
MAZZARELLA, LELIO MERLINO, ANTONELLO VERGARA, ALESSANDRO G. BONOMI M. LUBRANO A. RICCIO L. VITAGLIANO C. VERDE G. DI PRISCO Minimal Structural Requirements for Root Effect: The Crystal Structure of the Cathodic Hemoglobin Isolated from the Antarctic Fish Trematomus newnesi. |
author_facet |
MAZZARELLA, LELIO MERLINO, ANTONELLO VERGARA, ALESSANDRO G. BONOMI M. LUBRANO A. RICCIO L. VITAGLIANO C. VERDE G. DI PRISCO |
author_sort |
MAZZARELLA, LELIO |
title |
Minimal Structural Requirements for Root Effect: The Crystal Structure of the Cathodic Hemoglobin Isolated from the Antarctic Fish Trematomus newnesi. |
title_short |
Minimal Structural Requirements for Root Effect: The Crystal Structure of the Cathodic Hemoglobin Isolated from the Antarctic Fish Trematomus newnesi. |
title_full |
Minimal Structural Requirements for Root Effect: The Crystal Structure of the Cathodic Hemoglobin Isolated from the Antarctic Fish Trematomus newnesi. |
title_fullStr |
Minimal Structural Requirements for Root Effect: The Crystal Structure of the Cathodic Hemoglobin Isolated from the Antarctic Fish Trematomus newnesi. |
title_full_unstemmed |
Minimal Structural Requirements for Root Effect: The Crystal Structure of the Cathodic Hemoglobin Isolated from the Antarctic Fish Trematomus newnesi. |
title_sort |
minimal structural requirements for root effect: the crystal structure of the cathodic hemoglobin isolated from the antarctic fish trematomus newnesi. |
publishDate |
2006 |
url |
http://hdl.handle.net/11588/201965 https://doi.org/10.1002/prot.20709 |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/16299734 info:eu-repo/semantics/altIdentifier/wos/WOS:000234438800002 volume:62 issue:2 firstpage:316 lastpage:321 numberofpages:6 journal:PROTEINS http://hdl.handle.net/11588/201965 doi:10.1002/prot.20709 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-30144436769 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1002/prot.20709 |
container_title |
Proteins: Structure, Function, and Bioinformatics |
container_volume |
62 |
container_issue |
2 |
container_start_page |
316 |
op_container_end_page |
321 |
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1796943965497327616 |