Characterization of a Tc1-like transposon in the Antarctic ice-fish, Chionodraco hamatus.

• Published in: Gene
• Main Authors: CAPRIGLIONE, TERESA, ODIERNA G, CAPUTO V, CANAPA A, OLMO
• Other Authors: Capriglione, Teresa, Odierna, G, Caputo, V, Canapa, A, Olmo
• Format: Article in Journal/Newspaper
• Language: English
• Published: Elsevier BV:PO Box 211, 1000 AE Amsterdam Netherlands:011 31 20 4853757, 011 31 20 4853642, 011 31 20 4853641, EMAIL: [e-mail], INTERNET: http://www.elsevier.nl, Fax: [phone] 2002
• Subjects: Antarc*; Antarctic
• Online Access: http://hdl.handle.net/11588/135187; https://doi.org/10.1016/S0378-1119(02)00729-1

We report the presence of Tc1 transposon-like sequences in the Antarctic ice-fish Chionodraco hamatus, belonging to the Notothenioidei. The complete DNA sequence of these transposon-like elements is reduced in length compared to other Tc1 transposons, but it appears to share significant structural similarities with them. It contains a degenerate open reading frame, whose inferred 264 amino acid sequence shares sequence similarity with the 'aspartic acid, aspartic acid (35) glutamic acid' family of transposases, particularly those from Caenorhabditis species (sp.) and Drosophila sp. Southern blot analysis and polymerase chain reaction amplification indicate that Tc1 transposonlike sequences are present in other notothenioid species, though their amount can vary in the different lineages. (C) 2002 Elsevier Science B.V. All rights reserved.