Aspartate aminotransferase from the anctarctic bacterium Pseudolteromonas haloplanktis TAC 125. Clonong, expression, properties, and molecular modelling.

• Published in: European Journal of Biochemistry
• Main Authors: BIROLO, LEILA, TUTINO, MARIA LUISA, FONTANELLA, BIANCA, SANNIA, GIOVANNI, VINCI, FLORIANA, MARINO, GENNARO, GERDAY C, MAINOLFI K, PASCARELLA S
• Other Authors: Birolo, Leila, Tutino, MARIA LUISA, Fontanella, Bianca, Gerday, C, Mainolfi, K, Pascarella, S, Sannia, Giovanni, Vinci, Floriana, Marino, Gennaro
• Format: Article in Journal/Newspaper
• Language: English
• Published: 2000
• Subjects: aspartato amminotrasferasi; Pseudoalteromonas haloplankti; Antarctic bacterium; Antarctic; Tac; Antarc*
• Online Access: http://hdl.handle.net/11588/131504; https://doi.org/10.1046/j.1432-1327.2000.01299.x

The gene encoding aspartate aminotransferase from the psychrophilic bacterium Pseudoalteromonas haloplanktis TAC 125 was cloned, sequenced and overexpressed in Escherichia coli. The recombinant protein (PhAspAT) was characterized both at the structural and functional level in comparison with the E....