Hemoglobin structure/function and globin-gene evolution in the Arctic fish Liparis tunicatus
The importance of the Arctic, in contributing to the knowledge of the overall ensemble of adaptive processes influencing the evolution of marine organisms, calls for investigations on molecular adaptations in Arctic fish. Unlike the vast majority of Antarctic Notothenioidei, several Arctic species d...
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Online Access: | http://hdl.handle.net/11588/112687 https://doi.org/10.1016/j.gene.2007.06.002 |
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ftunivnapoliiris:oai:www.iris.unina.it:11588/112687 2024-09-09T19:08:28+00:00 Hemoglobin structure/function and globin-gene evolution in the Arctic fish Liparis tunicatus D. GIORDANO H. C. LEE J. PEISACH M. BALESTRIERI E. PARISI G. DI PRISCO C. VERDE VERGARA, ALESSANDRO MAZZARELLA, LELIO D., Giordano Vergara, Alessandro H. C., Lee J., Peisach M., Balestrieri Mazzarella, Lelio E., Parisi G., DI PRISCO C., Verde 2007 STAMPA http://hdl.handle.net/11588/112687 https://doi.org/10.1016/j.gene.2007.06.002 eng eng info:eu-repo/semantics/altIdentifier/wos/WOS:000252500500009 volume:406 firstpage:48 lastpage:58 numberofpages:11 journal:GENE http://hdl.handle.net/11588/112687 doi:10.1016/j.gene.2007.06.002 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-36749064392 info:eu-repo/semantics/article 2007 ftunivnapoliiris https://doi.org/10.1016/j.gene.2007.06.002 2024-06-17T15:19:22Z The importance of the Arctic, in contributing to the knowledge of the overall ensemble of adaptive processes influencing the evolution of marine organisms, calls for investigations on molecular adaptations in Arctic fish. Unlike the vast majority of Antarctic Notothenioidei, several Arctic species display high hemoglobin multiplicity. The blood of four species, the spotted wolffish of the family Anarhichadidae and three Gadidae, contains three functionally distinct major components. Similar to many Antarctic notothenioids, Arctic Liparis tunicatus (suborder Cottoidei, family Liparidae) has one major hemoglobin (Hb 1) accompanied by a minor component (Hb 2). This paper reports the structural and functional characterisation of Hb 1 of L. tunicatus. This hemoglobin shows low oxygen affinity, and pronounced Bohr and Root effects. The amino-acid sequence of the β chain displays an unusual substitution in NA2 (β2) at the phosphate-binding site, and the replacement of Val E11 (β67) with Ile. Similar to some Antarctic fish Hbs, electron paramagnetic resonance spectra reveal the formation of a ferric penta-coordinated species even at physiological pH. The amino-acid sequences have also been used to gain insight into the evolutionary history of globins of polar fish. L. tunicatus globins appear close to the notothenioid clades as predicted by teleostean phylogenies. Close phylogenetic relationships between Cottoidei and Notothenioidei, together with their life style, seem to be the main factor driving the globin-sequence evolution. Article in Journal/Newspaper Antarc* Antarctic Arctic IRIS Università degli Studi di Napoli Federico II Arctic Antarctic Gene 406 1-2 58 68 |
institution |
Open Polar |
collection |
IRIS Università degli Studi di Napoli Federico II |
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ftunivnapoliiris |
language |
English |
description |
The importance of the Arctic, in contributing to the knowledge of the overall ensemble of adaptive processes influencing the evolution of marine organisms, calls for investigations on molecular adaptations in Arctic fish. Unlike the vast majority of Antarctic Notothenioidei, several Arctic species display high hemoglobin multiplicity. The blood of four species, the spotted wolffish of the family Anarhichadidae and three Gadidae, contains three functionally distinct major components. Similar to many Antarctic notothenioids, Arctic Liparis tunicatus (suborder Cottoidei, family Liparidae) has one major hemoglobin (Hb 1) accompanied by a minor component (Hb 2). This paper reports the structural and functional characterisation of Hb 1 of L. tunicatus. This hemoglobin shows low oxygen affinity, and pronounced Bohr and Root effects. The amino-acid sequence of the β chain displays an unusual substitution in NA2 (β2) at the phosphate-binding site, and the replacement of Val E11 (β67) with Ile. Similar to some Antarctic fish Hbs, electron paramagnetic resonance spectra reveal the formation of a ferric penta-coordinated species even at physiological pH. The amino-acid sequences have also been used to gain insight into the evolutionary history of globins of polar fish. L. tunicatus globins appear close to the notothenioid clades as predicted by teleostean phylogenies. Close phylogenetic relationships between Cottoidei and Notothenioidei, together with their life style, seem to be the main factor driving the globin-sequence evolution. |
author2 |
D., Giordano Vergara, Alessandro H. C., Lee J., Peisach M., Balestrieri Mazzarella, Lelio E., Parisi G., DI PRISCO C., Verde |
format |
Article in Journal/Newspaper |
author |
D. GIORDANO H. C. LEE J. PEISACH M. BALESTRIERI E. PARISI G. DI PRISCO C. VERDE VERGARA, ALESSANDRO MAZZARELLA, LELIO |
spellingShingle |
D. GIORDANO H. C. LEE J. PEISACH M. BALESTRIERI E. PARISI G. DI PRISCO C. VERDE VERGARA, ALESSANDRO MAZZARELLA, LELIO Hemoglobin structure/function and globin-gene evolution in the Arctic fish Liparis tunicatus |
author_facet |
D. GIORDANO H. C. LEE J. PEISACH M. BALESTRIERI E. PARISI G. DI PRISCO C. VERDE VERGARA, ALESSANDRO MAZZARELLA, LELIO |
author_sort |
D. GIORDANO |
title |
Hemoglobin structure/function and globin-gene evolution in the Arctic fish Liparis tunicatus |
title_short |
Hemoglobin structure/function and globin-gene evolution in the Arctic fish Liparis tunicatus |
title_full |
Hemoglobin structure/function and globin-gene evolution in the Arctic fish Liparis tunicatus |
title_fullStr |
Hemoglobin structure/function and globin-gene evolution in the Arctic fish Liparis tunicatus |
title_full_unstemmed |
Hemoglobin structure/function and globin-gene evolution in the Arctic fish Liparis tunicatus |
title_sort |
hemoglobin structure/function and globin-gene evolution in the arctic fish liparis tunicatus |
publishDate |
2007 |
url |
http://hdl.handle.net/11588/112687 https://doi.org/10.1016/j.gene.2007.06.002 |
geographic |
Arctic Antarctic |
geographic_facet |
Arctic Antarctic |
genre |
Antarc* Antarctic Arctic |
genre_facet |
Antarc* Antarctic Arctic |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000252500500009 volume:406 firstpage:48 lastpage:58 numberofpages:11 journal:GENE http://hdl.handle.net/11588/112687 doi:10.1016/j.gene.2007.06.002 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-36749064392 |
op_doi |
https://doi.org/10.1016/j.gene.2007.06.002 |
container_title |
Gene |
container_volume |
406 |
container_issue |
1-2 |
container_start_page |
58 |
op_container_end_page |
68 |
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1809822701865402368 |