Structure and expression of genes involved in transport and storage of iron in red-blooded and hemoglobin-less Antarctic Notothenioids.
Antarctic notothenioids are characterized by a drastic reduction of the hemoglobin content, a condition that reaches its extreme in icefish that, following a gene deletion event, are completely devoid of hemoglobin. To answer the question on what type of adaptive changes occurred in icefish to preve...
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ftunivnapoliiris:oai:www.iris.unina.it:11588/110724 2024-09-09T19:03:40+00:00 Structure and expression of genes involved in transport and storage of iron in red-blooded and hemoglobin-less Antarctic Notothenioids. SCUDIERO, ROSARIA F. TRINCHELLA M. RIGGIO E. PARISI Scudiero, Rosaria F., Trinchella M., Riggio E., Parisi 2007 STAMPA http://hdl.handle.net/11588/110724 eng eng info:eu-repo/semantics/altIdentifier/wos/WOS:000248152800001 volume:397 firstpage:1 lastpage:11 numberofpages:11 journal:GENE http://hdl.handle.net/11588/110724 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-34250898026 info:eu-repo/semantics/closedAccess Ceruloplasmin Divalent metal transporter Ferritin Iron metabolism mRNA expression Transferrin Antarctic fish info:eu-repo/semantics/article 2007 ftunivnapoliiris 2024-06-17T15:19:22Z Antarctic notothenioids are characterized by a drastic reduction of the hemoglobin content, a condition that reaches its extreme in icefish that, following a gene deletion event, are completely devoid of hemoglobin. To answer the question on what type of adaptive changes occurred in icefish to prevent accumulation of potentially dangerous ferrous iron, we investigated the genes of four proteins known to play a key role in iron metabolism. For this purpose, we cloned and sequenced the cDNAs encoding ceruloplasmin, transferrin, ferritin and divalent metal transporter 1. While the inferred amino acid sequences of transferrin from different Antarctic fish species showed a high level of similarity with the homologous proteins from other species, ceruloplasmin sequence featured amino acid substitutions affecting a copper binding site. Another peculiarity was the presence in subunit H of the icefish ferritin of the two sets of sites involved in iron oxidation and iron mineralization, which in mammals are located on two distinct ferritin subunits. Significant differences in the expression levels of the four genes were found between hemoglobinless and red-blooded notothenioids. An increased expression of ceruloplasmin mRNA in icefish was interpreted as a compensatory mechanism to prevent accumulation of ferrous iron in hemoglobinless fish. In icefish, the amounts of ferritin H-chain mRNA measured in liver, blood and head kidney were lower than in the same organs of the red-blooded fish. In the spleen of both fishes, the expression levels of ferritin H-chain were significantly lower than in the spleen of a “pink-blooded” notothenioid with an intermediate hemoglobin content. Finally, the amount of divalent metal transporter mRNA measured in the head-kidney was lower in the icefish than in the same organ of its red-blooded counterpart. These results indicate that the loss of hemoglobin in icefish is accompanied by remodulation of the iron metabolism. Article in Journal/Newspaper Antarc* Antarctic Icefish IRIS Università degli Studi di Napoli Federico II Antarctic |
institution |
Open Polar |
collection |
IRIS Università degli Studi di Napoli Federico II |
op_collection_id |
ftunivnapoliiris |
language |
English |
topic |
Ceruloplasmin Divalent metal transporter Ferritin Iron metabolism mRNA expression Transferrin Antarctic fish |
spellingShingle |
Ceruloplasmin Divalent metal transporter Ferritin Iron metabolism mRNA expression Transferrin Antarctic fish SCUDIERO, ROSARIA F. TRINCHELLA M. RIGGIO E. PARISI Structure and expression of genes involved in transport and storage of iron in red-blooded and hemoglobin-less Antarctic Notothenioids. |
topic_facet |
Ceruloplasmin Divalent metal transporter Ferritin Iron metabolism mRNA expression Transferrin Antarctic fish |
description |
Antarctic notothenioids are characterized by a drastic reduction of the hemoglobin content, a condition that reaches its extreme in icefish that, following a gene deletion event, are completely devoid of hemoglobin. To answer the question on what type of adaptive changes occurred in icefish to prevent accumulation of potentially dangerous ferrous iron, we investigated the genes of four proteins known to play a key role in iron metabolism. For this purpose, we cloned and sequenced the cDNAs encoding ceruloplasmin, transferrin, ferritin and divalent metal transporter 1. While the inferred amino acid sequences of transferrin from different Antarctic fish species showed a high level of similarity with the homologous proteins from other species, ceruloplasmin sequence featured amino acid substitutions affecting a copper binding site. Another peculiarity was the presence in subunit H of the icefish ferritin of the two sets of sites involved in iron oxidation and iron mineralization, which in mammals are located on two distinct ferritin subunits. Significant differences in the expression levels of the four genes were found between hemoglobinless and red-blooded notothenioids. An increased expression of ceruloplasmin mRNA in icefish was interpreted as a compensatory mechanism to prevent accumulation of ferrous iron in hemoglobinless fish. In icefish, the amounts of ferritin H-chain mRNA measured in liver, blood and head kidney were lower than in the same organs of the red-blooded fish. In the spleen of both fishes, the expression levels of ferritin H-chain were significantly lower than in the spleen of a “pink-blooded” notothenioid with an intermediate hemoglobin content. Finally, the amount of divalent metal transporter mRNA measured in the head-kidney was lower in the icefish than in the same organ of its red-blooded counterpart. These results indicate that the loss of hemoglobin in icefish is accompanied by remodulation of the iron metabolism. |
author2 |
Scudiero, Rosaria F., Trinchella M., Riggio E., Parisi |
format |
Article in Journal/Newspaper |
author |
SCUDIERO, ROSARIA F. TRINCHELLA M. RIGGIO E. PARISI |
author_facet |
SCUDIERO, ROSARIA F. TRINCHELLA M. RIGGIO E. PARISI |
author_sort |
SCUDIERO, ROSARIA |
title |
Structure and expression of genes involved in transport and storage of iron in red-blooded and hemoglobin-less Antarctic Notothenioids. |
title_short |
Structure and expression of genes involved in transport and storage of iron in red-blooded and hemoglobin-less Antarctic Notothenioids. |
title_full |
Structure and expression of genes involved in transport and storage of iron in red-blooded and hemoglobin-less Antarctic Notothenioids. |
title_fullStr |
Structure and expression of genes involved in transport and storage of iron in red-blooded and hemoglobin-less Antarctic Notothenioids. |
title_full_unstemmed |
Structure and expression of genes involved in transport and storage of iron in red-blooded and hemoglobin-less Antarctic Notothenioids. |
title_sort |
structure and expression of genes involved in transport and storage of iron in red-blooded and hemoglobin-less antarctic notothenioids. |
publishDate |
2007 |
url |
http://hdl.handle.net/11588/110724 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Icefish |
genre_facet |
Antarc* Antarctic Icefish |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000248152800001 volume:397 firstpage:1 lastpage:11 numberofpages:11 journal:GENE http://hdl.handle.net/11588/110724 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-34250898026 |
op_rights |
info:eu-repo/semantics/closedAccess |
_version_ |
1809817681792073728 |