The sex determination of the oyster Crassostrea gigas : from phenotype to underlying molecular factors
Knowledge about the mode of reproduction and the sex determination of the cupped oyster Crassostrea gigas remains limited. As a sequential hermaphroditic species, sex determination takes place several times during its lifetime. As part of this thesis, the sexual phenotypes of the first 6 years of li...
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Format: | Doctoral or Postdoctoral Thesis |
Language: | French |
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ftunivnantes:oai:HAL:tel-03150111v1 2023-05-15T15:57:49+02:00 The sex determination of the oyster Crassostrea gigas : from phenotype to underlying molecular factors Le déterminisme sexuel de l'huître crassostrea gigas : du phénotype aux facteurs moléculaires sous-jacents Broquard, Coralie Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA) Normandie Université Anne-Sophie Martinez 2019-12-12 https://tel.archives-ouvertes.fr/tel-03150111 https://tel.archives-ouvertes.fr/tel-03150111/document https://tel.archives-ouvertes.fr/tel-03150111/file/sygal_fusion_28314-broquard-coralie.pdf fr fre HAL CCSD NNT: 2019NORMC264 tel-03150111 https://tel.archives-ouvertes.fr/tel-03150111 https://tel.archives-ouvertes.fr/tel-03150111/document https://tel.archives-ouvertes.fr/tel-03150111/file/sygal_fusion_28314-broquard-coralie.pdf info:eu-repo/semantics/OpenAccess https://tel.archives-ouvertes.fr/tel-03150111 Sciences agricoles. Normandie Université, 2019. Français. ⟨NNT : 2019NORMC264⟩ Sex determination Hermaphroditism Growth Molecular factors C. gigas Déterminisme sexuel Facteurs moléculaires [SDV.SA]Life Sciences [q-bio]/Agricultural sciences info:eu-repo/semantics/doctoralThesis Theses 2019 ftunivnantes 2022-07-12T23:26:20Z Knowledge about the mode of reproduction and the sex determination of the cupped oyster Crassostrea gigas remains limited. As a sequential hermaphroditic species, sex determination takes place several times during its lifetime. As part of this thesis, the sexual phenotypes of the first 6 years of life of oysters were identified in two cohorts. Thus, the sex ratio was biased towards females from the first sexual maturity, as well as for all subsequent years. After six years of sexing, 42%, 32%, 19%, 5%, 1% and 0.1% of oysters showed 0, 1, 2, 2, 3, 4 and 5 sex changes. The frequency of sex changes also decreased with age (34% between years 1-2 to 9% between years 5-6). This thesis work has also sought to determine the influence of sex and sex change on the morphological parameters of individuals. Thus, a sexual dimorphism was identified for the total weight as well as the length, width and thickness of the shell, in favour of female oysters, including those that have changed sex in the male to female direction. Finally, a transcriptomic analysis of the gonad of individuals with contrasted phenotypes ("true" females and "true" males) was to provide further knowledge on the molecular factors of sex determination. Thus, this approach made it possible (i) to identify the expression patterns of homologs of sex-determining genes, over the entire period of this mechanism and (ii) to identify new molecular actors of interest, overexpressed specifically in one sex during sex determination. Each expression pattern obtained in "true" males or "true" females could thus be interpreted in the light of the most probable future phenotype of the sex, despite sequential hermaphroditism. This approach allowed to refine hypotheses about the role of various factors in determining the sex of this cupped oyster. Le savoir relatif au mode de reproduction et au déterminisme sexuel de l’huître creuse Crassostrea gigas demeure limité. Etant une espèce hermaphrodite séquentielle, la détermination de son sexe a lieu plusieurs fois au cours ... Doctoral or Postdoctoral Thesis Crassostrea gigas Université de Nantes: HAL-UNIV-NANTES |
institution |
Open Polar |
collection |
Université de Nantes: HAL-UNIV-NANTES |
op_collection_id |
ftunivnantes |
language |
French |
topic |
Sex determination Hermaphroditism Growth Molecular factors C. gigas Déterminisme sexuel Facteurs moléculaires [SDV.SA]Life Sciences [q-bio]/Agricultural sciences |
spellingShingle |
Sex determination Hermaphroditism Growth Molecular factors C. gigas Déterminisme sexuel Facteurs moléculaires [SDV.SA]Life Sciences [q-bio]/Agricultural sciences Broquard, Coralie The sex determination of the oyster Crassostrea gigas : from phenotype to underlying molecular factors |
topic_facet |
Sex determination Hermaphroditism Growth Molecular factors C. gigas Déterminisme sexuel Facteurs moléculaires [SDV.SA]Life Sciences [q-bio]/Agricultural sciences |
description |
Knowledge about the mode of reproduction and the sex determination of the cupped oyster Crassostrea gigas remains limited. As a sequential hermaphroditic species, sex determination takes place several times during its lifetime. As part of this thesis, the sexual phenotypes of the first 6 years of life of oysters were identified in two cohorts. Thus, the sex ratio was biased towards females from the first sexual maturity, as well as for all subsequent years. After six years of sexing, 42%, 32%, 19%, 5%, 1% and 0.1% of oysters showed 0, 1, 2, 2, 3, 4 and 5 sex changes. The frequency of sex changes also decreased with age (34% between years 1-2 to 9% between years 5-6). This thesis work has also sought to determine the influence of sex and sex change on the morphological parameters of individuals. Thus, a sexual dimorphism was identified for the total weight as well as the length, width and thickness of the shell, in favour of female oysters, including those that have changed sex in the male to female direction. Finally, a transcriptomic analysis of the gonad of individuals with contrasted phenotypes ("true" females and "true" males) was to provide further knowledge on the molecular factors of sex determination. Thus, this approach made it possible (i) to identify the expression patterns of homologs of sex-determining genes, over the entire period of this mechanism and (ii) to identify new molecular actors of interest, overexpressed specifically in one sex during sex determination. Each expression pattern obtained in "true" males or "true" females could thus be interpreted in the light of the most probable future phenotype of the sex, despite sequential hermaphroditism. This approach allowed to refine hypotheses about the role of various factors in determining the sex of this cupped oyster. Le savoir relatif au mode de reproduction et au déterminisme sexuel de l’huître creuse Crassostrea gigas demeure limité. Etant une espèce hermaphrodite séquentielle, la détermination de son sexe a lieu plusieurs fois au cours ... |
author2 |
Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA) Normandie Université Anne-Sophie Martinez |
format |
Doctoral or Postdoctoral Thesis |
author |
Broquard, Coralie |
author_facet |
Broquard, Coralie |
author_sort |
Broquard, Coralie |
title |
The sex determination of the oyster Crassostrea gigas : from phenotype to underlying molecular factors |
title_short |
The sex determination of the oyster Crassostrea gigas : from phenotype to underlying molecular factors |
title_full |
The sex determination of the oyster Crassostrea gigas : from phenotype to underlying molecular factors |
title_fullStr |
The sex determination of the oyster Crassostrea gigas : from phenotype to underlying molecular factors |
title_full_unstemmed |
The sex determination of the oyster Crassostrea gigas : from phenotype to underlying molecular factors |
title_sort |
sex determination of the oyster crassostrea gigas : from phenotype to underlying molecular factors |
publisher |
HAL CCSD |
publishDate |
2019 |
url |
https://tel.archives-ouvertes.fr/tel-03150111 https://tel.archives-ouvertes.fr/tel-03150111/document https://tel.archives-ouvertes.fr/tel-03150111/file/sygal_fusion_28314-broquard-coralie.pdf |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
https://tel.archives-ouvertes.fr/tel-03150111 Sciences agricoles. Normandie Université, 2019. Français. ⟨NNT : 2019NORMC264⟩ |
op_relation |
NNT: 2019NORMC264 tel-03150111 https://tel.archives-ouvertes.fr/tel-03150111 https://tel.archives-ouvertes.fr/tel-03150111/document https://tel.archives-ouvertes.fr/tel-03150111/file/sygal_fusion_28314-broquard-coralie.pdf |
op_rights |
info:eu-repo/semantics/OpenAccess |
_version_ |
1766393509779079168 |