Temporal dynamics of lacustrine microbial eukaryotes inferred from paleogenetic approaches : tracking the impacts of climate change and nutrients enrichment
Eutrophication and climate warming are key factors governing lake functioning. However, there is a lack of knowledge about the response of microbial eukaryotic communities to these forcing factors even though microbial eukaryotes represent a huge taxonomic and functional diversity within lacustrine...
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ftunivnantes:oai:HAL:tel-01517017v1 2023-05-15T16:30:27+02:00 Temporal dynamics of lacustrine microbial eukaryotes inferred from paleogenetic approaches : tracking the impacts of climate change and nutrients enrichment Dynamique temporelle des communautés microbiennes eucaryotes en lien avec les forçages climatiques et anthropiques : approche paléolimnologique basée sur le séquençage massif d'ADN sédimentaire Capo, Éric Centre Alpin de Recherche sur les Réseaux Trophiques et Ecosystèmes Limniques (CARRTEL) Institut National de la Recherche Agronomique (INRA)-Université Savoie Mont Blanc (USMB Université de Savoie Université de Chambéry ) Université Grenoble Alpes Isabelle Domaizon Fabien Arnaud Didier Debroas 2016-12-19 https://theses.hal.science/tel-01517017 https://theses.hal.science/tel-01517017/document https://theses.hal.science/tel-01517017/file/CAPO_Eric_2016_archivage.pdf fr fre HAL CCSD NNT: 2016GREAA032 tel-01517017 https://theses.hal.science/tel-01517017 https://theses.hal.science/tel-01517017/document https://theses.hal.science/tel-01517017/file/CAPO_Eric_2016_archivage.pdf info:eu-repo/semantics/OpenAccess https://theses.hal.science/tel-01517017 Biodiversité et Ecologie. Université Grenoble Alpes, 2016. Français. ⟨NNT : 2016GREAA032⟩ Microbial eukaryotes High-Throughput sequencing Sedimentary DNA Paleolimnology Eutrophication Climate warming Microbes eucaryotes Séquençage massif ADN sédimentaire Paléolimnologie Eutrophisation Réchauffement climatique [SDE.BE]Environmental Sciences/Biodiversity and Ecology info:eu-repo/semantics/doctoralThesis Theses 2016 ftunivnantes 2023-03-08T06:49:02Z Eutrophication and climate warming are key factors governing lake functioning. However, there is a lack of knowledge about the response of microbial eukaryotic communities to these forcing factors even though microbial eukaryotes represent a huge taxonomic and functional diversity within lacustrine trophic networks. Paleolimnology has a well-established reputation for providing valuable insights into the drivers of biological assemblages over long time scales. The emergence of DNA analyses of lake sediments opens up many new opportunities for the reconstruction of past lacustrine biodiversity, including taxa that do not leave distinct morphological fossils. The present work aimed (i) to gain knowledge about the preservation of microbial eukaryotes DNA in lacustrine sediments (ii) to apply DNA-based methods to dated sediments in order to reveal the long-term dynamics (centennial to millennial) of microbial eukaryotes related to climatic and anthropogenic changes. The results obtained for Lake Bourget demonstrated the good efficiency of planktonic DNA archiving in recent sediments for most of microbial groups (chrysophyceae, chytrids, chlorophytes, cercozoa, ciliates, dinophyceae …). In complement, the use of a unique collection of freeze cores of varved sediment (Lake Nylandssjön, Sweden) allowed to assess the effects of diagenetic processes on microbial eukaryotes DNA occurring during the first years of burying. While the richness of the microbial eukaryotic community was not impacted, modifications were detected on the community structure during the first 15 years after deposition, then the DNA signal became stable. The paleoecological approach was applied to quantify centennial to millennial-scale dynamics on two deep peri-alpine lakes selected for their contrasted trophic history (lakes Bourget and Annecy, France) and two lakes with contrasted typologies (Lake Bourget, France and Lake Igaliku, Greenland). The results showed that some community rearrangements were concomitant with climate events (i.e. medieval ... Doctoral or Postdoctoral Thesis Greenland Igaliku Université de Nantes: HAL-UNIV-NANTES Greenland Igaliku ENVELOPE(-45.421,-45.421,60.988,60.988) |
institution |
Open Polar |
collection |
Université de Nantes: HAL-UNIV-NANTES |
op_collection_id |
ftunivnantes |
language |
French |
topic |
Microbial eukaryotes High-Throughput sequencing Sedimentary DNA Paleolimnology Eutrophication Climate warming Microbes eucaryotes Séquençage massif ADN sédimentaire Paléolimnologie Eutrophisation Réchauffement climatique [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
spellingShingle |
Microbial eukaryotes High-Throughput sequencing Sedimentary DNA Paleolimnology Eutrophication Climate warming Microbes eucaryotes Séquençage massif ADN sédimentaire Paléolimnologie Eutrophisation Réchauffement climatique [SDE.BE]Environmental Sciences/Biodiversity and Ecology Capo, Éric Temporal dynamics of lacustrine microbial eukaryotes inferred from paleogenetic approaches : tracking the impacts of climate change and nutrients enrichment |
topic_facet |
Microbial eukaryotes High-Throughput sequencing Sedimentary DNA Paleolimnology Eutrophication Climate warming Microbes eucaryotes Séquençage massif ADN sédimentaire Paléolimnologie Eutrophisation Réchauffement climatique [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
description |
Eutrophication and climate warming are key factors governing lake functioning. However, there is a lack of knowledge about the response of microbial eukaryotic communities to these forcing factors even though microbial eukaryotes represent a huge taxonomic and functional diversity within lacustrine trophic networks. Paleolimnology has a well-established reputation for providing valuable insights into the drivers of biological assemblages over long time scales. The emergence of DNA analyses of lake sediments opens up many new opportunities for the reconstruction of past lacustrine biodiversity, including taxa that do not leave distinct morphological fossils. The present work aimed (i) to gain knowledge about the preservation of microbial eukaryotes DNA in lacustrine sediments (ii) to apply DNA-based methods to dated sediments in order to reveal the long-term dynamics (centennial to millennial) of microbial eukaryotes related to climatic and anthropogenic changes. The results obtained for Lake Bourget demonstrated the good efficiency of planktonic DNA archiving in recent sediments for most of microbial groups (chrysophyceae, chytrids, chlorophytes, cercozoa, ciliates, dinophyceae …). In complement, the use of a unique collection of freeze cores of varved sediment (Lake Nylandssjön, Sweden) allowed to assess the effects of diagenetic processes on microbial eukaryotes DNA occurring during the first years of burying. While the richness of the microbial eukaryotic community was not impacted, modifications were detected on the community structure during the first 15 years after deposition, then the DNA signal became stable. The paleoecological approach was applied to quantify centennial to millennial-scale dynamics on two deep peri-alpine lakes selected for their contrasted trophic history (lakes Bourget and Annecy, France) and two lakes with contrasted typologies (Lake Bourget, France and Lake Igaliku, Greenland). The results showed that some community rearrangements were concomitant with climate events (i.e. medieval ... |
author2 |
Centre Alpin de Recherche sur les Réseaux Trophiques et Ecosystèmes Limniques (CARRTEL) Institut National de la Recherche Agronomique (INRA)-Université Savoie Mont Blanc (USMB Université de Savoie Université de Chambéry ) Université Grenoble Alpes Isabelle Domaizon Fabien Arnaud Didier Debroas |
format |
Doctoral or Postdoctoral Thesis |
author |
Capo, Éric |
author_facet |
Capo, Éric |
author_sort |
Capo, Éric |
title |
Temporal dynamics of lacustrine microbial eukaryotes inferred from paleogenetic approaches : tracking the impacts of climate change and nutrients enrichment |
title_short |
Temporal dynamics of lacustrine microbial eukaryotes inferred from paleogenetic approaches : tracking the impacts of climate change and nutrients enrichment |
title_full |
Temporal dynamics of lacustrine microbial eukaryotes inferred from paleogenetic approaches : tracking the impacts of climate change and nutrients enrichment |
title_fullStr |
Temporal dynamics of lacustrine microbial eukaryotes inferred from paleogenetic approaches : tracking the impacts of climate change and nutrients enrichment |
title_full_unstemmed |
Temporal dynamics of lacustrine microbial eukaryotes inferred from paleogenetic approaches : tracking the impacts of climate change and nutrients enrichment |
title_sort |
temporal dynamics of lacustrine microbial eukaryotes inferred from paleogenetic approaches : tracking the impacts of climate change and nutrients enrichment |
publisher |
HAL CCSD |
publishDate |
2016 |
url |
https://theses.hal.science/tel-01517017 https://theses.hal.science/tel-01517017/document https://theses.hal.science/tel-01517017/file/CAPO_Eric_2016_archivage.pdf |
long_lat |
ENVELOPE(-45.421,-45.421,60.988,60.988) |
geographic |
Greenland Igaliku |
geographic_facet |
Greenland Igaliku |
genre |
Greenland Igaliku |
genre_facet |
Greenland Igaliku |
op_source |
https://theses.hal.science/tel-01517017 Biodiversité et Ecologie. Université Grenoble Alpes, 2016. Français. ⟨NNT : 2016GREAA032⟩ |
op_relation |
NNT: 2016GREAA032 tel-01517017 https://theses.hal.science/tel-01517017 https://theses.hal.science/tel-01517017/document https://theses.hal.science/tel-01517017/file/CAPO_Eric_2016_archivage.pdf |
op_rights |
info:eu-repo/semantics/OpenAccess |
_version_ |
1766020184742559744 |